ID   MER_ARCFU               Reviewed;         332 AA.
AC   O29196;
DT   28-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=5,10-methylenetetrahydromethanopterin reductase {ECO:0000255|HAMAP-Rule:MF_01091};
DE            EC=1.5.98.2 {ECO:0000255|HAMAP-Rule:MF_01091};
DE   AltName: Full=Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase {ECO:0000255|HAMAP-Rule:MF_01091};
DE   AltName: Full=Methylene-H(4)MPT reductase {ECO:0000255|HAMAP-Rule:MF_01091};
GN   Name=mer {ECO:0000255|HAMAP-Rule:MF_01091}; OrderedLocusNames=AF_1066;
OS   Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC
OS   100126 / VC-16).
OC   Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales; Archaeoglobaceae;
OC   Archaeoglobus.
OX   NCBI_TaxID=224325;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16;
RX   PubMed=9389475; DOI=10.1038/37052;
RA   Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A.,
RA   Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L.,
RA   Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D.,
RA   Quackenbush J., Lee N.H., Sutton G.G., Gill S.R., Kirkness E.F.,
RA   Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N.,
RA   Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R.,
RA   Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D.,
RA   Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P.,
RA   Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M.,
RA   Smith H.O., Woese C.R., Venter J.C.;
RT   "The complete genome sequence of the hyperthermophilic, sulphate-reducing
RT   archaeon Archaeoglobus fulgidus.";
RL   Nature 390:364-370(1997).
CC   -!- FUNCTION: Catalyzes the oxidation of methyl-H(4)MPT to methylene-
CC       H(4)MPT. {ECO:0000255|HAMAP-Rule:MF_01091}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-methyl-5,6,7,8-tetrahydromethanopterin + H(+) + oxidized
CC         coenzyme F420-(gamma-L-Glu)(n) = 5,10-
CC         methylenetetrahydromethanopterin + reduced coenzyme F420-(gamma-L-
CC         Glu)(n); Xref=Rhea:RHEA:21144, Rhea:RHEA-COMP:12939, Rhea:RHEA-
CC         COMP:14378, ChEBI:CHEBI:15378, ChEBI:CHEBI:57818, ChEBI:CHEBI:58116,
CC         ChEBI:CHEBI:133980, ChEBI:CHEBI:139511; EC=1.5.98.2;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01091};
CC   -!- PATHWAY: Metabolic intermediate metabolism; lactate oxidation.
CC       {ECO:0000255|HAMAP-Rule:MF_01091}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01091}.
CC   -!- SIMILARITY: Belongs to the mer family. {ECO:0000255|HAMAP-
CC       Rule:MF_01091}.
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DR   EMBL; AE000782; AAB90174.1; -; Genomic_DNA.
DR   PIR; B69383; B69383.
DR   RefSeq; WP_010878566.1; NC_000917.1.
DR   AlphaFoldDB; O29196; -.
DR   SMR; O29196; -.
DR   STRING; 224325.AF_1066; -.
DR   EnsemblBacteria; AAB90174; AAB90174; AF_1066.
DR   GeneID; 24794676; -.
DR   KEGG; afu:AF_1066; -.
DR   eggNOG; arCOG02410; Archaea.
DR   HOGENOM; CLU_027853_5_3_2; -.
DR   OMA; VCWAAEA; -.
DR   OrthoDB; 46136at2157; -.
DR   PhylomeDB; O29196; -.
DR   BioCyc; MetaCyc:AF_RS05405-MON; -.
DR   UniPathway; UPA00701; -.
DR   Proteomes; UP000002199; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0018537; F:coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   GO; GO:0019516; P:lactate oxidation; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.20.20.30; -; 1.
DR   HAMAP; MF_01091; F420_mer; 1.
DR   InterPro; IPR011251; Luciferase-like_dom.
DR   InterPro; IPR036661; Luciferase-like_sf.
DR   InterPro; IPR019946; MeH4methanopterin_reductase.
DR   Pfam; PF00296; Bac_luciferase; 1.
DR   SUPFAM; SSF51679; SSF51679; 1.
DR   TIGRFAMs; TIGR03555; F420_mer; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; One-carbon metabolism; Oxidoreductase; Reference proteome.
FT   CHAIN           1..332
FT                   /note="5,10-methylenetetrahydromethanopterin reductase"
FT                   /id="PRO_0000084805"
SQ   SEQUENCE   332 AA;  36051 MW;  EA4264DF2727E4D9 CRC64;
     MKFGIEFVPD MKYYELEYYV KLAEDSGFDY TWITDHYNNR NVYSMLTILA LKTRTIKLGP
     GVTNPYHISP ALTASAIGTI NEISGGRAVL GIGAGDKVTF ERIGITWEKP LKRMREAVEI
     IRQLTEGKAV KYDGEIFKFN GAKLGFKPGS IPIYIGAQGP KMLQLAAELG DGVLINASHP
     KDFEVAKENI DAGLAKAGKS RDAFDTVAYA SMSVDKDRDK ARNAARIVVA FIVAGSPPTV
     LERHGLSEDA VNAVREALNN AFTKGDWGGV AKSVTDEMID IFSISGTPDD VIERINELSK
     AGVTQVVAGS PIGPDKKKSI QLIGKEIIPK LK