ID   MER_METAC               Reviewed;         328 AA.
AC   Q8TJP7;
DT   28-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 111.
DE   RecName: Full=5,10-methylenetetrahydromethanopterin reductase {ECO:0000255|HAMAP-Rule:MF_01091};
DE            EC=1.5.98.2 {ECO:0000255|HAMAP-Rule:MF_01091};
DE   AltName: Full=Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase {ECO:0000255|HAMAP-Rule:MF_01091};
DE   AltName: Full=Methylene-H(4)MPT reductase {ECO:0000255|HAMAP-Rule:MF_01091};
GN   Name=mer {ECO:0000255|HAMAP-Rule:MF_01091}; OrderedLocusNames=MA_3733;
OS   Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 /
OS   C2A).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=188937;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A;
RX   PubMed=11932238; DOI=10.1101/gr.223902;
RA   Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W.,
RA   Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J.,
RA   Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.,
RA   McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D.,
RA   Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R.,
RA   Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J.,
RA   Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A.,
RA   White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H.,
RA   Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V.,
RA   Zinder S.H., Lander E., Metcalf W.W., Birren B.;
RT   "The genome of Methanosarcina acetivorans reveals extensive metabolic and
RT   physiological diversity.";
RL   Genome Res. 12:532-542(2002).
CC   -!- FUNCTION: Catalyzes the reversible reduction of methylene-H(4)MPT to
CC       methyl-H(4)MPT. {ECO:0000255|HAMAP-Rule:MF_01091}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-methyl-5,6,7,8-tetrahydromethanopterin + H(+) + oxidized
CC         coenzyme F420-(gamma-L-Glu)(n) = 5,10-
CC         methylenetetrahydromethanopterin + reduced coenzyme F420-(gamma-L-
CC         Glu)(n); Xref=Rhea:RHEA:21144, Rhea:RHEA-COMP:12939, Rhea:RHEA-
CC         COMP:14378, ChEBI:CHEBI:15378, ChEBI:CHEBI:57818, ChEBI:CHEBI:58116,
CC         ChEBI:CHEBI:133980, ChEBI:CHEBI:139511; EC=1.5.98.2;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01091};
CC   -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); methyl-
CC       coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step
CC       1/2. {ECO:0000255|HAMAP-Rule:MF_01091}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01091}.
CC   -!- SIMILARITY: Belongs to the mer family. {ECO:0000255|HAMAP-
CC       Rule:MF_01091}.
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DR   EMBL; AE010299; AAM07085.1; -; Genomic_DNA.
DR   RefSeq; WP_011023637.1; NC_003552.1.
DR   AlphaFoldDB; Q8TJP7; -.
DR   SMR; Q8TJP7; -.
DR   STRING; 188937.MA_3733; -.
DR   EnsemblBacteria; AAM07085; AAM07085; MA_3733.
DR   GeneID; 1475626; -.
DR   KEGG; mac:MA_3733; -.
DR   HOGENOM; CLU_027853_5_3_2; -.
DR   InParanoid; Q8TJP7; -.
DR   OMA; VCWAAEA; -.
DR   OrthoDB; 46136at2157; -.
DR   PhylomeDB; Q8TJP7; -.
DR   UniPathway; UPA00640; UER00697.
DR   Proteomes; UP000002487; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0018537; F:coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.20.20.30; -; 1.
DR   HAMAP; MF_01091; F420_mer; 1.
DR   InterPro; IPR011251; Luciferase-like_dom.
DR   InterPro; IPR036661; Luciferase-like_sf.
DR   InterPro; IPR019946; MeH4methanopterin_reductase.
DR   Pfam; PF00296; Bac_luciferase; 1.
DR   SUPFAM; SSF51679; SSF51679; 1.
DR   TIGRFAMs; TIGR03555; F420_mer; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methanogenesis; One-carbon metabolism; Oxidoreductase;
KW   Reference proteome.
FT   CHAIN           1..328
FT                   /note="5,10-methylenetetrahydromethanopterin reductase"
FT                   /id="PRO_0000084806"
SQ   SEQUENCE   328 AA;  34720 MW;  C57ABD6B163CBD4E CRC64;
     MKFGIEFVPS DPALKIAYYA KLSEQQGFDY VWITDHYNNR DVYSTLTVLA LNTNSIKIGS
     GVTNSYTRNP AITASSIASI AEISGGRAVL GLGPGDKATF DAMGIAWEKP LATTKEAIQA
     IRDFIAGKKV SMDGEMVKFA GAKLAFKAGN VPIYMGAQGP KMLELAGEVA DGVLINASHP
     KDFEVAVEQI RKGAEKVGRD PSEVDVTAYA CFSIDKDPAK AINAAKVVVA FIVAGSPDLV
     LERHGIPVDA KKQIGDAIAK GDFGALMGGL VTPQMIEAFA ICGTPEDCMK RIKDLEAIGV
     TQIVAGSPIG PEKEKAIKLI GKEIIAKM