ID   MER_METJA               Reviewed;         331 AA.
AC   Q58929;
DT   28-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 2.
DT   25-MAY-2022, entry version 118.
DE   RecName: Full=5,10-methylenetetrahydromethanopterin reductase {ECO:0000255|HAMAP-Rule:MF_01091};
DE            EC=1.5.98.2 {ECO:0000255|HAMAP-Rule:MF_01091};
DE   AltName: Full=Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase {ECO:0000255|HAMAP-Rule:MF_01091};
DE   AltName: Full=Methylene-H(4)MPT reductase {ECO:0000255|HAMAP-Rule:MF_01091};
GN   Name=mer {ECO:0000255|HAMAP-Rule:MF_01091}; OrderedLocusNames=MJ1534;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
CC   -!- FUNCTION: Catalyzes the reversible reduction of methylene-H(4)MPT to
CC       methyl-H(4)MPT. {ECO:0000255|HAMAP-Rule:MF_01091}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-methyl-5,6,7,8-tetrahydromethanopterin + H(+) + oxidized
CC         coenzyme F420-(gamma-L-Glu)(n) = 5,10-
CC         methylenetetrahydromethanopterin + reduced coenzyme F420-(gamma-L-
CC         Glu)(n); Xref=Rhea:RHEA:21144, Rhea:RHEA-COMP:12939, Rhea:RHEA-
CC         COMP:14378, ChEBI:CHEBI:15378, ChEBI:CHEBI:57818, ChEBI:CHEBI:58116,
CC         ChEBI:CHEBI:133980, ChEBI:CHEBI:139511; EC=1.5.98.2;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01091};
CC   -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); methyl-
CC       coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step
CC       1/2. {ECO:0000255|HAMAP-Rule:MF_01091}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01091}.
CC   -!- SIMILARITY: Belongs to the mer family. {ECO:0000255|HAMAP-
CC       Rule:MF_01091}.
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DR   EMBL; L77117; AAB99555.1; -; Genomic_DNA.
DR   RefSeq; WP_010871058.1; NC_000909.1.
DR   AlphaFoldDB; Q58929; -.
DR   SMR; Q58929; -.
DR   STRING; 243232.MJ_1534; -.
DR   PRIDE; Q58929; -.
DR   EnsemblBacteria; AAB99555; AAB99555; MJ_1534.
DR   GeneID; 1452442; -.
DR   KEGG; mja:MJ_1534; -.
DR   eggNOG; arCOG02410; Archaea.
DR   HOGENOM; CLU_027853_5_3_2; -.
DR   InParanoid; Q58929; -.
DR   OMA; VCWAAEA; -.
DR   OrthoDB; 46136at2157; -.
DR   PhylomeDB; Q58929; -.
DR   UniPathway; UPA00640; UER00697.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0018537; F:coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.20.20.30; -; 1.
DR   HAMAP; MF_01091; F420_mer; 1.
DR   InterPro; IPR011251; Luciferase-like_dom.
DR   InterPro; IPR036661; Luciferase-like_sf.
DR   InterPro; IPR019946; MeH4methanopterin_reductase.
DR   Pfam; PF00296; Bac_luciferase; 1.
DR   SUPFAM; SSF51679; SSF51679; 1.
DR   TIGRFAMs; TIGR03555; F420_mer; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methanogenesis; One-carbon metabolism; Oxidoreductase;
KW   Reference proteome.
FT   CHAIN           1..331
FT                   /note="5,10-methylenetetrahydromethanopterin reductase"
FT                   /id="PRO_0000084807"
SQ   SEQUENCE   331 AA;  35353 MW;  367B5DFD1EB6EBBD CRC64;
     MKFGIEFVPN EPIQKLCYYV KLAEDNGFEY CWITDHYNNR NVYMALTAIA MNTNKIKLGP
     GVTNPYVRSP AITASAIATL DELSGGRAVL GIGPGDKATF DALGIEWVKP VTTLKESIEV
     IRKLLAGERV SYEGKVVKIA GAALAVKPIQ KAVPVYMGAQ GPKMLETAGM IADGVLINAS
     NPKDFEAAIP LIKKGAEAAG RSMDEIDVAA YACMSVDKNA DKAKQAAVPV VAFIAAGSPP
     VVLERHGIDM EKVEAIRNAL KSGNFPEAFK NVDDTMLEAF SIYGTPEDVV EKCKKLAEMG
     VTQIVAGSPI GPNKETAIKL IGKKVIPALK E