MER_METTH
ID MER_METTH Reviewed; 321 AA.
AC O27784; Q50540;
DT 28-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=5,10-methylenetetrahydromethanopterin reductase;
DE EC=1.5.98.2 {ECO:0000269|PubMed:2298726};
DE AltName: Full=Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase;
DE AltName: Full=Methylene-H(4)MPT reductase;
GN Name=mer {ECO:0000303|PubMed:8522533};
GN OrderedLocusNames=MTH_1752 {ECO:0000312|EMBL:AAB86222.1};
OS Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX NCBI_TaxID=187420;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX PubMed=8522533; DOI=10.1128/jb.177.24.7238-7244.1995;
RA Noelling J., Pihl T.D., Reeve J.N.;
RT "Cloning, sequencing, and growth phase-dependent transcription of the
RT coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin reductase-
RT encoding genes from Methanobacterium thermoautotrophicum delta H and
RT Methanopyrus kandleri.";
RL J. Bacteriol. 177:7238-7244(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA Reeve J.N.;
RT "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT functional analysis and comparative genomics.";
RL J. Bacteriol. 179:7135-7155(1997).
RN [3]
RP FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, AND PATHWAY.
RC STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX PubMed=2298726; DOI=10.1016/s0021-9258(19)39907-7;
RA te Broemmelstroet B.W., Hensgens C.M.H., Keltjens J.T., van der Drift C.,
RA Vogels G.D.;
RT "Purification and properties of 5,10-methylenetetrahydromethanopterin
RT reductase, a coenzyme F420-dependent enzyme, from Methanobacterium
RT thermoautotrophicum strain delta H.";
RL J. Biol. Chem. 265:1852-1857(1990).
CC -!- FUNCTION: Catalyzes the reversible reduction of methylene-H(4)MPT to
CC methyl-H(4)MPT. {ECO:0000269|PubMed:2298726}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5-methyl-5,6,7,8-tetrahydromethanopterin + H(+) + oxidized
CC coenzyme F420-(gamma-L-Glu)(n) = 5,10-
CC methylenetetrahydromethanopterin + reduced coenzyme F420-(gamma-L-
CC Glu)(n); Xref=Rhea:RHEA:21144, Rhea:RHEA-COMP:12939, Rhea:RHEA-
CC COMP:14378, ChEBI:CHEBI:15378, ChEBI:CHEBI:57818, ChEBI:CHEBI:58116,
CC ChEBI:CHEBI:133980, ChEBI:CHEBI:139511; EC=1.5.98.2;
CC Evidence={ECO:0000269|PubMed:2298726};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Optimum pH is 7. {ECO:0000269|PubMed:2298726};
CC Temperature dependence:
CC Optimum temperature is 40 degrees Celsius.
CC {ECO:0000269|PubMed:2298726};
CC -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); methyl-
CC coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step
CC 1/2. {ECO:0000269|PubMed:2298726}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- INDUCTION: Increase in abundance from the earliest stages of
CC exponential growth, reaching a maximum level at the mid-exponential
CC growth phase. {ECO:0000269|PubMed:8522533}.
CC -!- MISCELLANEOUS: Oxygen-stable. {ECO:0000269|PubMed:2298726}.
CC -!- SIMILARITY: Belongs to the mer family. {ECO:0000305}.
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DR EMBL; U31568; AAA92087.1; -; Genomic_DNA.
DR EMBL; AE000666; AAB86222.1; -; Genomic_DNA.
DR PIR; D69101; D69101.
DR RefSeq; WP_010877358.1; NC_000916.1.
DR AlphaFoldDB; O27784; -.
DR SMR; O27784; -.
DR STRING; 187420.MTH_1752; -.
DR PRIDE; O27784; -.
DR EnsemblBacteria; AAB86222; AAB86222; MTH_1752.
DR GeneID; 1470837; -.
DR KEGG; mth:MTH_1752; -.
DR PATRIC; fig|187420.15.peg.1712; -.
DR HOGENOM; CLU_027853_5_3_2; -.
DR OMA; VCWAAEA; -.
DR BioCyc; MetaCyc:MERMAUTO-MON; -.
DR UniPathway; UPA00640; UER00697.
DR Proteomes; UP000005223; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0018537; F:coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR Gene3D; 3.20.20.30; -; 1.
DR HAMAP; MF_01091; F420_mer; 1.
DR InterPro; IPR011251; Luciferase-like_dom.
DR InterPro; IPR036661; Luciferase-like_sf.
DR InterPro; IPR019946; MeH4methanopterin_reductase.
DR Pfam; PF00296; Bac_luciferase; 1.
DR SUPFAM; SSF51679; SSF51679; 1.
DR TIGRFAMs; TIGR03555; F420_mer; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Methanogenesis; One-carbon metabolism; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..321
FT /note="5,10-methylenetetrahydromethanopterin reductase"
FT /id="PRO_0000084810"
FT CONFLICT 313
FT /note="L -> H (in Ref. 1; AAA92087)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 321 AA; 33479 MW; F1E7B93500E3C852 CRC64;
MKFGIEFVPN EPIEKIVKLV KLAEDVGFEY AWITDHYNNK NVYETLALIA EGTETIKLGP
GVTNPYVRSP AITASAIATL DELSNGRATL GIGPGDKATF DALGIEWVKP VSTIRDAIAM
MRTLLAGEKT ESGAQLMGVK AVQEKIPIYM GAQGPMMLKT AGEISDGALI NASNPKDFEA
AVPLIKEGAE SAGKSLSDID VAAYTCCSID EDSAAAANAA KIVVAFIAAG SPPPVFERHG
LPADTGAKFG ELLGKGDFGG AIGAVDDALM EAFSVVGTPD EFIPKIEALG EMGVTQYVAG
SPIGPDKEKS IKLLGEVIAS F
