MER_METTI
ID MER_METTI Reviewed; 326 AA.
AC Q9UXP0;
DT 28-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 79.
DE RecName: Full=5,10-methylenetetrahydromethanopterin reductase {ECO:0000255|HAMAP-Rule:MF_01091};
DE EC=1.5.98.2 {ECO:0000255|HAMAP-Rule:MF_01091};
DE AltName: Full=Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase {ECO:0000255|HAMAP-Rule:MF_01091};
DE AltName: Full=Methylene-H(4)MPT reductase {ECO:0000255|HAMAP-Rule:MF_01091};
GN Name=mer {ECO:0000255|HAMAP-Rule:MF_01091}; Synonyms=ffdA;
OS Methanolobus tindarius.
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanosarcinales; Methanosarcinaceae; Methanolobus.
OX NCBI_TaxID=2221;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 35996 / DSM 2278 / OCM 150 / Tindari 3;
RX PubMed=9933933; DOI=10.1111/j.1574-6968.1999.tb13399.x;
RA Westenberg D.J., Braune A., Ruppert C., Mueller V., Herzberg C.,
RA Gottschalk G., Blaut M.;
RT "The F420H2-dehydrogenase from Methanolobus tindarius: cloning of the ffd
RT operon and expression of the genes in Escherichia coli.";
RL FEMS Microbiol. Lett. 170:389-398(1999).
CC -!- FUNCTION: Catalyzes the reversible reduction of methylene-H(4)MPT to
CC methyl-H(4)MPT. {ECO:0000255|HAMAP-Rule:MF_01091}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5-methyl-5,6,7,8-tetrahydromethanopterin + H(+) + oxidized
CC coenzyme F420-(gamma-L-Glu)(n) = 5,10-
CC methylenetetrahydromethanopterin + reduced coenzyme F420-(gamma-L-
CC Glu)(n); Xref=Rhea:RHEA:21144, Rhea:RHEA-COMP:12939, Rhea:RHEA-
CC COMP:14378, ChEBI:CHEBI:15378, ChEBI:CHEBI:57818, ChEBI:CHEBI:58116,
CC ChEBI:CHEBI:133980, ChEBI:CHEBI:139511; EC=1.5.98.2;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01091};
CC -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); methyl-
CC coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step
CC 1/2. {ECO:0000255|HAMAP-Rule:MF_01091}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01091}.
CC -!- SIMILARITY: Belongs to the mer family. {ECO:0000255|HAMAP-
CC Rule:MF_01091}.
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DR EMBL; AJ011519; CAB56639.1; -; Genomic_DNA.
DR PIR; T45226; T45226.
DR AlphaFoldDB; Q9UXP0; -.
DR SMR; Q9UXP0; -.
DR UniPathway; UPA00640; UER00697.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0018537; F:coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR Gene3D; 3.20.20.30; -; 1.
DR HAMAP; MF_01091; F420_mer; 1.
DR InterPro; IPR011251; Luciferase-like_dom.
DR InterPro; IPR036661; Luciferase-like_sf.
DR InterPro; IPR019946; MeH4methanopterin_reductase.
DR Pfam; PF00296; Bac_luciferase; 1.
DR SUPFAM; SSF51679; SSF51679; 1.
DR TIGRFAMs; TIGR03555; F420_mer; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Methanogenesis; One-carbon metabolism; Oxidoreductase.
FT CHAIN 1..326
FT /note="5,10-methylenetetrahydromethanopterin reductase"
FT /id="PRO_0000084811"
SQ SEQUENCE 326 AA; 34043 MW; 16F3AB9733A45D82 CRC64;
MTFGIEFVPS DPVLKIAHYA KLAEQQGFDN VWITDHYNNR DVYSTLTVLA MNTNSIKLGT
GVTNPYTRNA AITASSIGSL NEISGGRAIL GLGPGEQATF DAMGISWEQP LTTTKESIAA
IRGFLAGEKV TMDGDMIKFG GAKMAFKAGD VPIYMGAQGP KMLELAGEVS DGVLINASHP
KDFEVAVKQI AAGAKKAGRD PKEVDVAAYA CFSIDKDAAK AKSAAQIVVA FIVAGSPDMV
LERHGIDPAA KADIGGAIAK GDFGALMGGM VTDSMMDAFS ICGTPDDCKA RINELLDIGV
TQIVAGSPIG PNKEKAIKLI GKEIIG
