MES13_ARATH
ID MES13_ARATH Reviewed; 444 AA.
AC F4IE65; Q9FZC2;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 69.
DE RecName: Full=Putative methylesterase 13, chloroplastic {ECO:0000303|PubMed:18467465};
DE Short=AtMES13 {ECO:0000303|PubMed:18467465};
DE EC=3.1.1.- {ECO:0000250|UniProtKB:Q9SG92};
DE Flags: Precursor;
GN Name=MES13 {ECO:0000303|PubMed:18467465};
GN OrderedLocusNames=At1g26360 {ECO:0000312|Araport:AT1G26360};
GN ORFNames=T1K7.26 {ECO:0000312|EMBL:AAF98579.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP GENE FAMILY.
RX PubMed=18467465; DOI=10.1104/pp.108.118224;
RA Yang Y., Xu R., Ma C.J., Vlot A.C., Klessig D.F., Pichersky E.;
RT "Inactive methyl indole-3-acetic acid ester can be hydrolyzed and activated
RT by several esterases belonging to the AtMES esterase family of
RT Arabidopsis.";
RL Plant Physiol. 147:1034-1045(2008).
CC -!- FUNCTION: Putative methylesterase.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Methylesterase
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF98579.1; Type=Erroneous gene model prediction; Note=The predicted gene has been split into 2 genes: At1g26355 and At1g26360.; Evidence={ECO:0000305};
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DR EMBL; AC013427; AAF98579.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE30682.1; -; Genomic_DNA.
DR EMBL; BX816109; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; NP_173960.2; NM_102400.3.
DR AlphaFoldDB; F4IE65; -.
DR SMR; F4IE65; -.
DR STRING; 3702.AT1G26360.1; -.
DR ESTHER; arath-F4IE65; Hydroxynitrile_lyase.
DR MEROPS; S33.A69; -.
DR PaxDb; F4IE65; -.
DR PRIDE; F4IE65; -.
DR EnsemblPlants; AT1G26360.1; AT1G26360.1; AT1G26360.
DR GeneID; 839178; -.
DR Gramene; AT1G26360.1; AT1G26360.1; AT1G26360.
DR KEGG; ath:AT1G26360; -.
DR Araport; AT1G26360; -.
DR TAIR; locus:2197975; AT1G26360.
DR eggNOG; ENOG502S15T; Eukaryota.
DR HOGENOM; CLU_046066_8_1_1; -.
DR InParanoid; F4IE65; -.
DR OMA; DRIYPNS; -.
DR OrthoDB; 923240at2759; -.
DR PRO; PR:F4IE65; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4IE65; baseline and differential.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0080030; F:methyl indole-3-acetate esterase activity; IBA:GO_Central.
DR GO; GO:0080032; F:methyl jasmonate esterase activity; IBA:GO_Central.
DR GO; GO:0080031; F:methyl salicylate esterase activity; IBA:GO_Central.
DR GO; GO:0009694; P:jasmonic acid metabolic process; IBA:GO_Central.
DR GO; GO:0009696; P:salicylic acid metabolic process; IBA:GO_Central.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR000073; AB_hydrolase_1.
DR InterPro; IPR045889; MES/HNL.
DR PANTHER; PTHR10992; PTHR10992; 1.
DR Pfam; PF12697; Abhydrolase_6; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Hydrolase; Plastid; Reference proteome; Transit peptide.
FT TRANSIT 1..60
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 61..444
FT /note="Putative methylesterase 13, chloroplastic"
FT /id="PRO_0000418187"
FT DOMAIN 190..310
FT /note="AB hydrolase-1"
FT /evidence="ECO:0000255"
FT REGION 1..32
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 49..90
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 124..176
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 49..65
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 66..80
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 124..170
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 264
FT /note="Acyl-ester intermediate"
FT /evidence="ECO:0000250|UniProtKB:Q6RYA0"
FT ACT_SITE 390
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:Q6RYA0"
FT ACT_SITE 418
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:Q6RYA0"
SQ SEQUENCE 444 AA; 49093 MW; 48CE0F245D463573 CRC64;
MGNSFTCISH EQEQRPKKSS GGGGNNSGKY KYVRRLSLMP SFRRRTLLPS LSCSGSSSTS
SSKKGGIKAK TKKIRERHHH HHQDHEKDSH IIQEQTLAAT NLLFNQTPRN SNSVVPPSFR
RSTSVVYPSA QPSGTSSGPV SAVQTPKKSS AGFVRSSSSR QRSSTDPMIK PNQLVDKELN
KVEGSETKRF VLVHGGGFGA WCWYKTITLL EKHGFQVDAV ELTGSGVSSI DTNNITSLAH
YSKPLLHFFE SLKPTEKVIL VGHDFGGACM SYAMEMFPTK IAKAVFISAA MLANGQSTLD
LFNQQLGSND LMQQAQIFLY ANGKKNPPTA VDFDRSLLRD FLFNQSPPKD LALASVSIRP
IPFAPVSEKV HVSEKNYGSI RRFYIKTMED YAVPVLLQEA MIKLNPPEQV FQLKGSDHAP
FFSRPQSLNK ILVEISQIPF KKSS
