MES15_ARATH
ID MES15_ARATH Reviewed; 444 AA.
AC F4I0K9; Q9C979; Q9LQA0;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 65.
DE RecName: Full=Putative methylesterase 15, chloroplastic {ECO:0000303|PubMed:18467465};
DE Short=AtMES15 {ECO:0000303|PubMed:18467465};
DE EC=3.1.1.- {ECO:0000250|UniProtKB:Q9SG92};
DE AltName: Full=Protein ROOT HAIR SPECIFIC 9;
DE Flags: Precursor;
GN Name=MES15 {ECO:0000303|PubMed:18467465}; Synonyms=RHS9;
GN OrderedLocusNames=At1g69240 {ECO:0000312|Araport:AT1G69240};
GN ORFNames=F23O10.18 {ECO:0000312|EMBL:AAG52490.1},
GN F4N2.19 {ECO:0000312|EMBL:AAF27064.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP GENE FAMILY.
RX PubMed=18467465; DOI=10.1104/pp.108.118224;
RA Yang Y., Xu R., Ma C.J., Vlot A.C., Klessig D.F., Pichersky E.;
RT "Inactive methyl indole-3-acetic acid ester can be hydrolyzed and activated
RT by several esterases belonging to the AtMES esterase family of
RT Arabidopsis.";
RL Plant Physiol. 147:1034-1045(2008).
CC -!- FUNCTION: Putative methylesterase.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Methylesterase
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF27064.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAG52490.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BX816117; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
CC Sequence=BX816402; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
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DR EMBL; AC008262; AAF27064.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC018364; AAG52490.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE34900.1; -; Genomic_DNA.
DR EMBL; BX816117; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BX816402; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; D96716; D96716.
DR RefSeq; NP_177084.2; NM_105591.3.
DR AlphaFoldDB; F4I0K9; -.
DR SMR; F4I0K9; -.
DR STRING; 3702.AT1G69240.1; -.
DR ESTHER; arath-MES15; Hydroxynitrile_lyase.
DR MEROPS; S33.A69; -.
DR iPTMnet; F4I0K9; -.
DR PaxDb; F4I0K9; -.
DR PRIDE; F4I0K9; -.
DR ProteomicsDB; 250645; -.
DR EnsemblPlants; AT1G69240.1; AT1G69240.1; AT1G69240.
DR GeneID; 843255; -.
DR Gramene; AT1G69240.1; AT1G69240.1; AT1G69240.
DR KEGG; ath:AT1G69240; -.
DR Araport; AT1G69240; -.
DR TAIR; locus:2026434; AT1G69240.
DR eggNOG; ENOG502S15T; Eukaryota.
DR HOGENOM; CLU_046066_8_1_1; -.
DR InParanoid; F4I0K9; -.
DR OMA; EAMIKSN; -.
DR OrthoDB; 923240at2759; -.
DR PRO; PR:F4I0K9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4I0K9; baseline and differential.
DR Genevisible; F4I0K9; AT.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0080030; F:methyl indole-3-acetate esterase activity; IBA:GO_Central.
DR GO; GO:0080032; F:methyl jasmonate esterase activity; IBA:GO_Central.
DR GO; GO:0080031; F:methyl salicylate esterase activity; IBA:GO_Central.
DR GO; GO:0009694; P:jasmonic acid metabolic process; IBA:GO_Central.
DR GO; GO:0009696; P:salicylic acid metabolic process; IBA:GO_Central.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR000073; AB_hydrolase_1.
DR InterPro; IPR045889; MES/HNL.
DR PANTHER; PTHR10992; PTHR10992; 1.
DR Pfam; PF12697; Abhydrolase_6; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Hydrolase; Plastid; Reference proteome; Transit peptide.
FT TRANSIT 1..58
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 59..444
FT /note="Putative methylesterase 15, chloroplastic"
FT /id="PRO_0000418188"
FT DOMAIN 188..312
FT /note="AB hydrolase-1"
FT /evidence="ECO:0000255"
FT REGION 1..36
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 48..91
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..33
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 48..63
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 64..78
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 262
FT /note="Acyl-ester intermediate"
FT /evidence="ECO:0000250|UniProtKB:Q6RYA0"
FT ACT_SITE 390
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:Q6RYA0"
FT ACT_SITE 418
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:Q6RYA0"
SQ SEQUENCE 444 AA; 49329 MW; FC9C54AE4A61E641 CRC64;
MGNSLRCISQ EQDPNQKKPS SVVNGNSSEK HVRRLSLIPS FRRRTLLPSL SCSGSSTSST
SKKGGIKTKK KIRERHHQEQ HHHDHEKDSL IQDQTLAATN ILFSQTPRNS NSAPPFRRST
SVVYTQPPTA AVAASVGSVS GALTPKKSTY GYVRSSSNRQ RSSTDPVLKP NQLLDKELKV
EGAETKRFVL VHGGGFGAWC WYKTITLLEK HGFQVDAVDL TGSGVSSFDT NNITSLAQYV
KPLLHFFDTL KPTEKVILVG HDFGGACMSY AMEMYPSKIA KAIFISAAML ANAQSTLDLF
NQQPDSNYDL MEQVHLFLYA NGKKNPPTAV DFDRSLLRDF FFNQSPPKDV ALASVSMRPI
PFAPVVEKLH VSEKNYGSIR RFYIKTMEDD YAVPVSLQDA MIKSNPPEQV FHLKGSDHAP
FFSRPQSLNR ILVEISQLPP KKSS
