MES18_ARATH
ID MES18_ARATH Reviewed; 263 AA.
AC Q9LVL9;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Methylesterase 18 {ECO:0000303|PubMed:18467465};
DE Short=AtMES18 {ECO:0000303|PubMed:18467465};
DE EC=3.1.1.- {ECO:0000269|PubMed:18467465};
GN Name=MES18 {ECO:0000303|PubMed:18467465};
GN OrderedLocusNames=At5g58310 {ECO:0000312|Araport:AT5G58310};
GN ORFNames=MCK7.18 {ECO:0000312|EMBL:BAA96922.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RX PubMed=18467465; DOI=10.1104/pp.108.118224;
RA Yang Y., Xu R., Ma C.J., Vlot A.C., Klessig D.F., Pichersky E.;
RT "Inactive methyl indole-3-acetic acid ester can be hydrolyzed and activated
RT by several esterases belonging to the AtMES esterase family of
RT Arabidopsis.";
RL Plant Physiol. 147:1034-1045(2008).
CC -!- FUNCTION: Methylesterase shown to have methyl indole-3-acetic acid
CC (MeIAA) esterase activity in vitro. {ECO:0000269|PubMed:18467465}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + methyl (indol-3-yl)acetate = (indol-3-yl)acetate + H(+)
CC + methanol; Xref=Rhea:RHEA:32919, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:30854,
CC ChEBI:CHEBI:72782; Evidence={ECO:0000269|PubMed:18467465};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:32920;
CC Evidence={ECO:0000269|PubMed:18467465};
CC -!- PATHWAY: Plant hormone biosynthesis. {ECO:0000269|PubMed:18467465}.
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Methylesterase
CC family. {ECO:0000305}.
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DR EMBL; AB019228; BAA96922.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97032.1; -; Genomic_DNA.
DR EMBL; BT004145; AAO42166.1; -; mRNA.
DR EMBL; BT005672; AAO64092.1; -; mRNA.
DR EMBL; AY084976; AAM61536.1; -; mRNA.
DR RefSeq; NP_200639.1; NM_125216.3.
DR AlphaFoldDB; Q9LVL9; -.
DR SMR; Q9LVL9; -.
DR BioGRID; 21187; 2.
DR IntAct; Q9LVL9; 1.
DR STRING; 3702.AT5G58310.1; -.
DR ESTHER; arath-MES18; Hydroxynitrile_lyase.
DR PaxDb; Q9LVL9; -.
DR PRIDE; Q9LVL9; -.
DR ProteomicsDB; 250646; -.
DR EnsemblPlants; AT5G58310.1; AT5G58310.1; AT5G58310.
DR GeneID; 835943; -.
DR Gramene; AT5G58310.1; AT5G58310.1; AT5G58310.
DR KEGG; ath:AT5G58310; -.
DR Araport; AT5G58310; -.
DR TAIR; locus:2161223; AT5G58310.
DR eggNOG; ENOG502RZ4X; Eukaryota.
DR HOGENOM; CLU_046066_0_1_1; -.
DR InParanoid; Q9LVL9; -.
DR OMA; CWYKLAN; -.
DR OrthoDB; 923240at2759; -.
DR PhylomeDB; Q9LVL9; -.
DR BioCyc; ARA:AT5G58310-MON; -.
DR PRO; PR:Q9LVL9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LVL9; baseline and differential.
DR Genevisible; Q9LVL9; AT.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IDA:TAIR.
DR GO; GO:0080030; F:methyl indole-3-acetate esterase activity; IDA:TAIR.
DR GO; GO:0080032; F:methyl jasmonate esterase activity; IBA:GO_Central.
DR GO; GO:0080031; F:methyl salicylate esterase activity; IBA:GO_Central.
DR GO; GO:0009694; P:jasmonic acid metabolic process; IBA:GO_Central.
DR GO; GO:0009696; P:salicylic acid metabolic process; IBA:GO_Central.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR000073; AB_hydrolase_1.
DR InterPro; IPR045889; MES/HNL.
DR PANTHER; PTHR10992; PTHR10992; 1.
DR Pfam; PF12697; Abhydrolase_6; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 1: Evidence at protein level;
KW Hydrolase; Reference proteome.
FT CHAIN 1..263
FT /note="Methylesterase 18"
FT /id="PRO_0000418190"
FT ACT_SITE 80
FT /note="Acyl-ester intermediate"
FT /evidence="ECO:0000250|UniProtKB:Q6RYA0"
FT ACT_SITE 212
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:Q6RYA0"
FT ACT_SITE 240
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:Q6RYA0"
SQ SEQUENCE 263 AA; 29092 MW; 7C95DE7CFA3BBC4D CRC64;
MSEHHFVFVH GAGHGGWCWY KLANSLRDNG HKATCIDLKG AGINPTDPNT VSSLDDYDEP
LYAFLSQLPN DQKVILVSHS VGGGSMTAAM CLFPSKVSLA VYVAAAMVKP GTLIPERLKN
VMKICSGLIE EETEKIWDFT FGNGPQNLPT SIMMKPEYVR DKFYNESPME DYTLATTLLR
PAPVMAFIGI MDIPGAPETD KIPRVYVKTG KDHLFEPVLQ EVMLALWPPA HTFLLPDSDH
SAFFSQPQEL YQFLLQAASS LSP
