MES3_ARATH
ID MES3_ARATH Reviewed; 263 AA.
AC O80477;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Methylesterase 3 {ECO:0000303|PubMed:18467465};
DE Short=AtMES3 {ECO:0000303|PubMed:18467465};
DE EC=3.1.1.- {ECO:0000269|PubMed:18467465};
GN Name=MES3 {ECO:0000303|PubMed:18467465};
GN OrderedLocusNames=At2g23610 {ECO:0000312|Araport:AT2G23610};
GN ORFNames=F26B6.26 {ECO:0000312|EMBL:AAC23774.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY, FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RX PubMed=18467465; DOI=10.1104/pp.108.118224;
RA Yang Y., Xu R., Ma C.J., Vlot A.C., Klessig D.F., Pichersky E.;
RT "Inactive methyl indole-3-acetic acid ester can be hydrolyzed and activated
RT by several esterases belonging to the AtMES esterase family of
RT Arabidopsis.";
RL Plant Physiol. 147:1034-1045(2008).
CC -!- FUNCTION: Methylesterase shown to have carboxylesterase activity,
CC methyl indole-3-acetic acid (MeIAA) esterase activity and methyl
CC jasmonate (MeJA) esterase activity in vitro.
CC {ECO:0000269|PubMed:18467465}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + methyl (indol-3-yl)acetate = (indol-3-yl)acetate + H(+)
CC + methanol; Xref=Rhea:RHEA:32919, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:30854,
CC ChEBI:CHEBI:72782; Evidence={ECO:0000269|PubMed:18467465};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:32920;
CC Evidence={ECO:0000269|PubMed:18467465};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + methyl (-)-jasmonate = H(+) + jasmonate + methanol;
CC Xref=Rhea:RHEA:55372, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15929, ChEBI:CHEBI:17790, ChEBI:CHEBI:58431;
CC Evidence={ECO:0000269|PubMed:18467465};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55373;
CC Evidence={ECO:0000269|PubMed:18467465};
CC -!- PATHWAY: Plant hormone biosynthesis. {ECO:0000269|PubMed:18467465}.
CC -!- PATHWAY: Lipid metabolism; oxylipin biosynthesis.
CC {ECO:0000269|PubMed:18467465}.
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Methylesterase
CC family. {ECO:0000305}.
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DR EMBL; AC003040; AAC23774.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07472.1; -; Genomic_DNA.
DR EMBL; BT014901; AAT46030.1; -; mRNA.
DR EMBL; BT015031; AAT70482.1; -; mRNA.
DR PIR; T01150; T01150.
DR RefSeq; NP_179942.1; NM_127925.3.
DR AlphaFoldDB; O80477; -.
DR SMR; O80477; -.
DR BioGRID; 2245; 1.
DR STRING; 3702.AT2G23610.1; -.
DR ESTHER; arath-MES3; Hydroxynitrile_lyase.
DR iPTMnet; O80477; -.
DR PaxDb; O80477; -.
DR PRIDE; O80477; -.
DR ProteomicsDB; 232249; -.
DR EnsemblPlants; AT2G23610.1; AT2G23610.1; AT2G23610.
DR GeneID; 816893; -.
DR Gramene; AT2G23610.1; AT2G23610.1; AT2G23610.
DR KEGG; ath:AT2G23610; -.
DR Araport; AT2G23610; -.
DR TAIR; locus:2046862; AT2G23610.
DR eggNOG; ENOG502QR2J; Eukaryota.
DR HOGENOM; CLU_046066_0_1_1; -.
DR InParanoid; O80477; -.
DR OMA; MIANSGV; -.
DR OrthoDB; 923240at2759; -.
DR PhylomeDB; O80477; -.
DR BioCyc; ARA:AT2G23610-MON; -.
DR UniPathway; UPA00382; -.
DR PRO; PR:O80477; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O80477; baseline and differential.
DR Genevisible; O80477; AT.
DR GO; GO:0005773; C:vacuole; HDA:TAIR.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IDA:TAIR.
DR GO; GO:0080030; F:methyl indole-3-acetate esterase activity; IDA:TAIR.
DR GO; GO:0080032; F:methyl jasmonate esterase activity; IDA:TAIR.
DR GO; GO:0080031; F:methyl salicylate esterase activity; IBA:GO_Central.
DR GO; GO:0009694; P:jasmonic acid metabolic process; IBA:GO_Central.
DR GO; GO:0031408; P:oxylipin biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0009696; P:salicylic acid metabolic process; IBA:GO_Central.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR000073; AB_hydrolase_1.
DR InterPro; IPR045889; MES/HNL.
DR PANTHER; PTHR10992; PTHR10992; 1.
DR Pfam; PF12697; Abhydrolase_6; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 1: Evidence at protein level;
KW Hydrolase; Reference proteome.
FT CHAIN 1..263
FT /note="Methylesterase 3"
FT /id="PRO_0000418178"
FT ACT_SITE 85
FT /note="Acyl-ester intermediate"
FT /evidence="ECO:0000250|UniProtKB:Q6RYA0"
FT ACT_SITE 213
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:Q6RYA0"
FT ACT_SITE 241
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:Q6RYA0"
SQ SEQUENCE 263 AA; 29823 MW; D91B69A229E3DF09 CRC64;
MSEEERKQHV VLVHGACHGA WCWYKVKPQL EASGHRVTAV DLAASGIDMT RSITDISTCE
QYSEPLMQLM TSLPDDEKVV LVGHSLGGLS LAMAMDMFPT KISVSVFVTA MMPDTKHSPS
FVWDKLRKET SREEWLDTVF TSEKPDFPSE FWIFGPEFMA KNLYQLSPVQ DLELAKMLVR
ANPLIKKDMA ERRSFSEEGY GSVTRIFIVC GKDLVSPEDY QRSMISNFPP KEVMEIKDAD
HMPMFSKPQQ LCALLLEIAN KYA
