MES3_CAEEL
ID MES3_CAEEL Reviewed; 754 AA.
AC Q10665; P91324;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 28-MAR-2003, sequence version 2.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Polycomb protein mes-3;
DE AltName: Full=Maternal-effect sterile protein 3;
GN Name=mes-3 {ECO:0000312|WormBase:F54C1.3a};
GN ORFNames=F54C1.3 {ECO:0000312|WormBase:F54C1.3a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Bristol N2;
RX PubMed=8601481; DOI=10.1093/genetics/141.4.1383;
RA Paulsen J.E., Capowski E.E., Strome S.;
RT "Phenotypic and molecular analysis of mes-3, a maternal-effect gene
RT required for proliferation and viability of the germ line in C. elegans.";
RL Genetics 141:1383-1398(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP CHARACTERIZATION, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF CYS-283.
RX PubMed=11729149; DOI=10.1093/genetics/159.3.1007;
RA Xu L., Paulsen J., Yoo Y., Goodwin E.B., Strome S.;
RT "Caenorhabditis elegans MES-3 is a target of GLD-1 and functions
RT epigenetically in germline development.";
RL Genetics 159:1007-1017(2001).
RN [4]
RP IDENTIFICATION IN A COMPLEX WITH MES-2 AND MES-6.
RX PubMed=11320248; DOI=10.1073/pnas.081016198;
RA Xu L., Fong Y., Strome S.;
RT "The Caenorhabditis elegans maternal-effect sterile proteins, MES-2, MES-3,
RT and MES-6, are associated in a complex in embryos.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:5061-5066(2001).
RN [5]
RP FUNCTION.
RX PubMed=12077420; DOI=10.1126/science.1070790;
RA Fong Y., Bender L., Wang W., Strome S.;
RT "Regulation of the different chromatin states of autosomes and X
RT chromosomes in the germ line of C. elegans.";
RL Science 296:2235-2238(2002).
RN [6]
RP FUNCTION, AND IDENTIFICATION IN A COMPLEX WITH MES-2 AND MES-6.
RX PubMed=15380065; DOI=10.1016/j.cub.2004.08.062;
RA Bender L.B., Cao R., Zhang Y., Strome S.;
RT "The MES-2/MES-3/MES-6 complex and regulation of histone H3 methylation in
RT C. elegans.";
RL Curr. Biol. 14:1639-1643(2004).
RN [7]
RP FUNCTION, INTERACTION WITH NFYA-1, AND DISRUPTION PHENOTYPE.
RX PubMed=17574230; DOI=10.1016/j.ydbio.2007.05.021;
RA Deng H., Sun Y., Zhang Y., Luo X., Hou W., Yan L., Chen Y., Tian E.,
RA Han J., Zhang H.;
RT "Transcription factor NFY globally represses the expression of the C.
RT elegans Hox gene Abdominal-B homolog egl-5.";
RL Dev. Biol. 308:583-592(2007).
CC -!- FUNCTION: Component of a Polycomb group (PcG) complex. PcG proteins act
CC by forming multiprotein complexes, which are required to maintain the
CC transcriptionally repressive state of homeotic genes throughout
CC development. In association with the nfya-1-NF-Y complex, may play a
CC role in repressing the expression of the homeobox protein egl-5 in
CC tissues such as the head (PubMed:17574230). PcG proteins are not
CC required to initiate repression, but to maintain it during later stages
CC of development. The mes-2/mes-3/mes-6 complex may participate in the
CC global inactivation of the X chromosomes in germline cells. The complex
CC may act via methylation of histone H3 'Lys-27', rendering chromatin
CC heritably changed in its expressibility. This complex is required to
CC exclude mes-4 from the inactivated X-chromosomes in germline cells.
CC {ECO:0000269|PubMed:12077420, ECO:0000269|PubMed:15380065,
CC ECO:0000269|PubMed:17574230}.
CC -!- SUBUNIT: Forms a heterotrimeric complex with the Polycomb proteins mes-
CC 2 and mes-3. Does not interact with mes-4. Interacts with nyfa-1
CC (PubMed:17574230). {ECO:0000269|PubMed:11320248,
CC ECO:0000269|PubMed:15380065, ECO:0000269|PubMed:17574230}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: In adults, it is predominantly expressed in the
CC germline, and weakly expressed in intestinal cells.
CC -!- DEVELOPMENTAL STAGE: Expressed maternally and zygotically. Expressed in
CC all cells of early embryos. In late embryos and L1 larva, it is weakly
CC expressed, mainly in Z2 and Z3 cells. {ECO:0000269|PubMed:11729149}.
CC -!- INDUCTION: Repressed by GLD-1, which associates with the 3'-UTR of the
CC mRNA and prevents its translation.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in ectopic
CC expression of the homeobox protein egl-5 in the head region
CC (PubMed:17574230). This ectopic expression of egl-5 in the head region
CC is enhanced in a nfya-1 bp4 mutant background (PubMed:17574230).
CC {ECO:0000269|PubMed:17574230}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U34812; AAB01719.1; -; mRNA.
DR EMBL; BX284601; CCD67544.1; -; Genomic_DNA.
DR PIR; G87767; G87767.
DR PIR; S60464; S60464.
DR RefSeq; NP_001021492.1; NM_001026321.2.
DR AlphaFoldDB; Q10665; -.
DR BioGRID; 37584; 8.
DR ComplexPortal; CPX-368; Polycomb Repressive Complex 2.
DR IntAct; Q10665; 2.
DR STRING; 6239.F54C1.3a; -.
DR iPTMnet; Q10665; -.
DR PaxDb; Q10665; -.
DR EnsemblMetazoa; F54C1.3a.1; F54C1.3a.1; WBGene00003221.
DR EnsemblMetazoa; F54C1.3a.2; F54C1.3a.2; WBGene00003221.
DR GeneID; 172123; -.
DR KEGG; cel:CELE_F54C1.3; -.
DR UCSC; F54C1.3a; c. elegans.
DR CTD; 172123; -.
DR WormBase; F54C1.3a; CE11046; WBGene00003221; mes-3.
DR eggNOG; ENOG502R29G; Eukaryota.
DR HOGENOM; CLU_393916_0_0_1; -.
DR InParanoid; Q10665; -.
DR OMA; WANAVET; -.
DR OrthoDB; 1479008at2759; -.
DR PRO; PR:Q10665; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00003221; Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues.
DR ExpressionAtlas; Q10665; baseline and differential.
DR GO; GO:0000786; C:nucleosome; IDA:WormBase.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0031519; C:PcG protein complex; IPI:WormBase.
DR GO; GO:0016571; P:histone methylation; IDA:WormBase.
DR GO; GO:0010629; P:negative regulation of gene expression; IDA:ComplexPortal.
DR GO; GO:0010468; P:regulation of gene expression; IMP:UniProtKB.
PE 1: Evidence at protein level;
KW Developmental protein; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..754
FT /note="Polycomb protein mes-3"
FT /id="PRO_0000096439"
FT REGION 1..83
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 9..41
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 42..83
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 283
FT /note="C->Y: In BN21; temperature-sensitive allele, which
FT produces fertile progeny at permissive temperature (16
FT degrees Celsius) and sterile progeny at restrictive
FT temperature (25 degrees Celsius), probably due to a
FT mislocation into the cytoplasm."
FT /evidence="ECO:0000269|PubMed:11729149"
FT CONFLICT 264
FT /note="A -> V (in Ref. 1; AAB01719)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 754 AA; 86305 MW; B7B3EFCFAC0FA512 CRC64;
MTPATAEVKV GGRKRKNDEP VFIKTKQSRS ARREEEKENL LNKSLPSTPT SSEAGSSRES
SNPVTSSSRR KNPPTKLENI QKTLPTCSDG LEIRNYVKKY GLPEDNKFLV RNVFDKQLLF
GKKYVCRRRV IKSIDEFFPR LKTDAHRENG LYNFCTLQFM KISSWGSSMD EKIYVTSAAI
VQSYVIIVDD NDSVTCIENG ISVIPMANRT LPSISYNSSN VKNITLTGPE LKKAKNVYLA
VIVKRKTPKL GENRVGTRGK STRASFETAA SSENFQQLVR FGCTLMYDKD AEINCLNDGV
QRIILLDREE KPAYLKIFGK ADEPELENAW MNNVKHLEFI SDTNYNKEDS HLARLIFSSN
THNYFDRPDI SKFSPWQPRS RRTTLKSGIS VEPGTSCDED CGSQYEDTIT LKQLLLGTKK
LCGRRKRHLT RLTSRWPCFS VDPTKINSYF NETGVTLLEN SCSYRPGTQR SIPIKPTRVI
RVREETPVSV PIEVITIEDD SDDSPCFSAR NRAPGGTRKY GLDFIQSSGH MPYIRYVYMN
RANDLVPQLN GEENGKIEGL MEAYPMNNAH MYSDIEFLRK EKLNLPKNAQ KYGEMTILDY
PLTKTETDEY NKKMSVMNVK DFAVKPRKPI PTVGNTTLSL DLEGCPISTF SEIFFPSGKT
ELFNILCRHF YSEAGQRPGC FSAQWKSRVL TGFINKYHQY IFDMKLNSLF EKQINLIAMV
SPDWKLEDFE LPMVRYKQLK KEWKNRQRDI PSSN
