MES6_CAEEL
ID MES6_CAEEL Reviewed; 459 AA.
AC Q9GYS1; O16187;
DT 28-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 2.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Polycomb protein mes-6;
DE AltName: Full=ESC homolog;
DE AltName: Full=Maternal-effect sterile protein 6;
GN Name=mes-6 {ECO:0000312|WormBase:C09G4.5};
GN ORFNames=C09G4.5 {ECO:0000312|WormBase:C09G4.5};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, DEVELOPMENTAL STAGE,
RP AND MUTAGENESIS OF GLY-188.
RC STRAIN=Bristol N2;
RX PubMed=9609830; DOI=10.1242/dev.125.13.2469;
RA Korf I., Fan Y., Strome S.;
RT "The polycomb group in Caenorhabditis elegans and maternal control of
RT germline development.";
RL Development 125:2469-2478(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP IDENTIFICATION IN A COMPLEX WITH MES-2 AND MES-3.
RX PubMed=11320248; DOI=10.1073/pnas.081016198;
RA Xu L., Fong Y., Strome S.;
RT "The Caenorhabditis elegans maternal-effect sterile proteins, MES-2, MES-3,
RT and MES-6, are associated in a complex in embryos.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:5061-5066(2001).
RN [4]
RP FUNCTION.
RX PubMed=12077420; DOI=10.1126/science.1070790;
RA Fong Y., Bender L., Wang W., Strome S.;
RT "Regulation of the different chromatin states of autosomes and X
RT chromosomes in the germ line of C. elegans.";
RL Science 296:2235-2238(2002).
RN [5]
RP FUNCTION, AND IDENTIFICATION IN A COMPLEX WITH MES-2 AND MES-3.
RX PubMed=15380065; DOI=10.1016/j.cub.2004.08.062;
RA Bender L.B., Cao R., Zhang Y., Strome S.;
RT "The MES-2/MES-3/MES-6 complex and regulation of histone H3 methylation in
RT C. elegans.";
RL Curr. Biol. 14:1639-1643(2004).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17574230; DOI=10.1016/j.ydbio.2007.05.021;
RA Deng H., Sun Y., Zhang Y., Luo X., Hou W., Yan L., Chen Y., Tian E.,
RA Han J., Zhang H.;
RT "Transcription factor NFY globally represses the expression of the C.
RT elegans Hox gene Abdominal-B homolog egl-5.";
RL Dev. Biol. 308:583-592(2007).
RN [7]
RP FUNCTION.
RX PubMed=26365259; DOI=10.1016/j.cub.2015.07.051;
RA Mao H., Zhu C., Zong D., Weng C., Yang X., Huang H., Liu D., Feng X.,
RA Guang S.;
RT "The Nrde pathway mediates small-RNA-directed histone H3 lysine 27
RT trimethylation in Caenorhabditis elegans.";
RL Curr. Biol. 25:2398-2403(2015).
CC -!- FUNCTION: Polycomb group (PcG) protein. PcG proteins act by forming
CC multiprotein complexes, which are required to maintain the
CC transcriptionally repressive state of homeotic genes throughout
CC development. In association with the nfya-1-NF-Y complex, may play a
CC role in repressing the expression of the homeobox protein egl-5 in
CC tissues such as the head (PubMed:17574230). PcG proteins are not
CC required to initiate repression, but to maintain it during later stages
CC of development. The mes-2/mes-3/mes-6 complex may participate in the
CC global inactivation of the X chromosomes in germline cells. The complex
CC may act via methylation of histone H3 'Lys-27', rendering chromatin
CC heritably changed in its expressibility. This complex is required to
CC exclude mes-4 from the inactivated X-chromosomes in germline cells
CC (PubMed:12077420, PubMed:15380065). Required for small-RNA-induced
CC H3K27 trimethylation (PubMed:26365259). {ECO:0000269|PubMed:12077420,
CC ECO:0000269|PubMed:15380065, ECO:0000269|PubMed:17574230,
CC ECO:0000269|PubMed:26365259}.
CC -!- SUBUNIT: Interacts directly with the N-terminal domain of mes-2. Forms
CC a heterotrimeric complex with mes-2 and mes-3. Does not interact with
CC mes-4. {ECO:0000269|PubMed:11320248, ECO:0000269|PubMed:15380065}.
CC -!- INTERACTION:
CC Q9GYS1; O17514: mes-2; NbExp=5; IntAct=EBI-314965, EBI-11615731;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: In adults, it is predominantly expressed in the
CC germline, and weakly expressed in intestinal cells.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC Expressed in all cells of early embryos. In late embryos and L1 larva,
CC it is weakly expressed. {ECO:0000269|PubMed:9609830}.
CC -!- DISRUPTION PHENOTYPE: Double RNAi-mediated knockdown together with mes-
CC 2 RNAi results in ectopic expression of the homeobox protein egl-5 in
CC the head region (PubMed:17574230). This ectopic expression of egl-5 in
CC the head region is enhanced in a nfya-1 bp4 mutant background
CC (PubMed:17574230). In addition in this background in males, there is
CC ectopic expression of egl-5 in the mid-body region including in seam
CC cells and hypodermal nuclei, and there is ectopic ray formation
CC (PubMed:17574230). {ECO:0000269|PubMed:17574230}.
CC -!- SIMILARITY: Belongs to the WD repeat ESC family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF016224; AAC27121.1; -; Genomic_DNA.
DR EMBL; BX284604; CCD64001.1; -; Genomic_DNA.
DR RefSeq; NP_001021320.1; NM_001026149.4.
DR AlphaFoldDB; Q9GYS1; -.
DR SMR; Q9GYS1; -.
DR BioGRID; 42769; 11.
DR ComplexPortal; CPX-368; Polycomb Repressive Complex 2.
DR IntAct; Q9GYS1; 2.
DR STRING; 6239.C09G4.5; -.
DR EPD; Q9GYS1; -.
DR PaxDb; Q9GYS1; -.
DR PeptideAtlas; Q9GYS1; -.
DR EnsemblMetazoa; C09G4.5.1; C09G4.5.1; WBGene00003224.
DR GeneID; 177657; -.
DR UCSC; C09G4.5; c. elegans.
DR CTD; 177657; -.
DR WormBase; C09G4.5; CE24796; WBGene00003224; mes-6.
DR eggNOG; KOG1034; Eukaryota.
DR GeneTree; ENSGT00510000047334; -.
DR HOGENOM; CLU_032683_4_1_1; -.
DR InParanoid; Q9GYS1; -.
DR OMA; QTDVCVA; -.
DR OrthoDB; 1191277at2759; -.
DR PhylomeDB; Q9GYS1; -.
DR Reactome; R-CEL-212300; PRC2 methylates histones and DNA.
DR Reactome; R-CEL-2559580; Oxidative Stress Induced Senescence.
DR Reactome; R-CEL-8943724; Regulation of PTEN gene transcription.
DR PRO; PR:Q9GYS1; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00003224; Expressed in embryo and 3 other tissues.
DR GO; GO:0035098; C:ESC/E(Z) complex; IBA:GO_Central.
DR GO; GO:0000786; C:nucleosome; IDA:WormBase.
DR GO; GO:0031519; C:PcG protein complex; IPI:WormBase.
DR GO; GO:0016571; P:histone methylation; IDA:WormBase.
DR GO; GO:0010629; P:negative regulation of gene expression; IDA:ComplexPortal.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0010468; P:regulation of gene expression; IMP:UniProtKB.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR037352; Polycomb_EED.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR10253:SF5; PTHR10253:SF5; 1.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM00320; WD40; 4.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Nucleus; Reference proteome; Repeat; Repressor;
KW Transcription; Transcription regulation; WD repeat.
FT CHAIN 1..459
FT /note="Polycomb protein mes-6"
FT /id="PRO_0000051076"
FT REPEAT 146..186
FT /note="WD 1"
FT REPEAT 192..231
FT /note="WD 2"
FT REPEAT 305..346
FT /note="WD 3"
FT REPEAT 370..409
FT /note="WD 4"
FT REPEAT 415..454
FT /note="WD 5"
FT MUTAGEN 188
FT /note="G->E: In BN66; maternal-effect mutation. Progeny
FT show defects in gonad proliferation. Germ cell
FT degeneration."
FT /evidence="ECO:0000269|PubMed:9609830"
SQ SEQUENCE 459 AA; 51823 MW; 0524A674ABAB2671 CRC64;
MEHTKKFKSL NLHGFDRSTE DYGKRPFVLT AKLLEDQKKA IYGCAFNQYA GIDEEQAVAT
VGGSFLHMYS VPIDINNIEL QWSCNFPTDK SSKVEREESL FTVTWCYDTY EAENDRNPFK
VVTGGTLGHI YVIDYVSRKL SNRLRSVGWE INDIRTCPAN SNLIVCASSD QSIRIHHIRN
EACLIVIGGL ECHAGTILSV DWSTDGDFIL SCGFDHQLME WDLSVKQVKE HLERACKALH
QDKINVLTQS QDIPYVSKGT MRKSAVSRNI PDKEEDQLLE LHRELIPRPS CLLPIYTPSS
VSTDMHSDYV DCIRFLIGTN YALSKGCGNE KAIHFWRFGP PKGEVENRIH GNVLRPKSCT
TKFRTMNVPS GSAWFIKFAV DPRRRWLVCG GAGGSVMFFD LRNNEETNPT HTCSVGSRTV
RQASFSTCGR FLVLVTDEGF VCRFDRVSAS VDAKDLAKF
