MES7_ARATH
ID MES7_ARATH Reviewed; 260 AA.
AC O80472;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Methylesterase 7 {ECO:0000303|PubMed:18467465};
DE Short=AtMES7 {ECO:0000303|PubMed:18467465};
DE EC=3.1.1.- {ECO:0000269|PubMed:18467465};
GN Name=MES7 {ECO:0000303|PubMed:18467465};
GN OrderedLocusNames=At2g23560 {ECO:0000312|Araport:AT2G23560};
GN ORFNames=F26B6.21 {ECO:0000312|EMBL:AAC23783.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, ACTIVITY REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, AND INDUCTION
RP BY PATHOGEN.
RX PubMed=18643994; DOI=10.1111/j.1365-313x.2008.03618.x;
RA Vlot A.C., Liu P.P., Cameron R.K., Park S.W., Yang Y., Kumar D., Zhou F.,
RA Padukkavidana T., Gustafsson C., Pichersky E., Klessig D.F.;
RT "Identification of likely orthologs of tobacco salicylic acid-binding
RT protein 2 and their role in systemic acquired resistance in Arabidopsis
RT thaliana.";
RL Plant J. 56:445-456(2008).
RN [5]
RP GENE FAMILY, FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RX PubMed=18467465; DOI=10.1104/pp.108.118224;
RA Yang Y., Xu R., Ma C.J., Vlot A.C., Klessig D.F., Pichersky E.;
RT "Inactive methyl indole-3-acetic acid ester can be hydrolyzed and activated
RT by several esterases belonging to the AtMES esterase family of
RT Arabidopsis.";
RL Plant Physiol. 147:1034-1045(2008).
CC -!- FUNCTION: Methylesterase shown to have carboxylesterase activity,
CC methyl indole-3-acetic acid (MeIAA) esterase activity and methyl
CC salicylate (MeSA) esterase activity in vitro. Required to convert
CC methyl salicylate (MeSA) to salicylic acid (SA) as part of the signal
CC transduction pathways that activate systemic acquired resistance in
CC systemic tissue. MeSA is believed to be an inactive form that needs to
CC be demethylated to exert a biological effect.
CC {ECO:0000269|PubMed:18467465, ECO:0000269|PubMed:18643994}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + methyl (indol-3-yl)acetate = (indol-3-yl)acetate + H(+)
CC + methanol; Xref=Rhea:RHEA:32919, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:30854,
CC ChEBI:CHEBI:72782; Evidence={ECO:0000269|PubMed:18467465};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:32920;
CC Evidence={ECO:0000269|PubMed:18467465};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + methyl salicylate = H(+) + methanol + salicylate;
CC Xref=Rhea:RHEA:33611, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17790, ChEBI:CHEBI:30762, ChEBI:CHEBI:31832;
CC Evidence={ECO:0000269|PubMed:18467465};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33612;
CC Evidence={ECO:0000269|PubMed:18467465};
CC -!- ACTIVITY REGULATION: Esterase activity is down-regulated by salicylic
CC acid (SA). {ECO:0000269|PubMed:18643994}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC Vmax=12.82 nmol/min/ug enzyme with methyl salicylate (MeSA) as
CC substrate {ECO:0000269|PubMed:18643994};
CC -!- PATHWAY: Plant hormone biosynthesis. {ECO:0000269|PubMed:18467465}.
CC -!- INDUCTION: By pathogen infection. {ECO:0000269|PubMed:18643994}.
CC -!- MISCELLANEOUS: Expression of MES7 can restore systemic acquired
CC resistance in SAR-deficient tobacco plants.
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Methylesterase
CC family. {ECO:0000305}.
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DR EMBL; AC003040; AAC23783.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07466.1; -; Genomic_DNA.
DR EMBL; BT010709; AAR20766.1; -; mRNA.
DR EMBL; BT012414; AAS92330.1; -; mRNA.
DR PIR; T01145; T01145.
DR RefSeq; NP_179937.1; NM_127920.3.
DR AlphaFoldDB; O80472; -.
DR SMR; O80472; -.
DR STRING; 3702.AT2G23560.1; -.
DR ESTHER; arath-MES7; Hydroxynitrile_lyase.
DR MEROPS; S33.A70; -.
DR PaxDb; O80472; -.
DR PRIDE; O80472; -.
DR ProteomicsDB; 238961; -.
DR EnsemblPlants; AT2G23560.1; AT2G23560.1; AT2G23560.
DR GeneID; 816888; -.
DR Gramene; AT2G23560.1; AT2G23560.1; AT2G23560.
DR KEGG; ath:AT2G23560; -.
DR Araport; AT2G23560; -.
DR TAIR; locus:2046793; AT2G23560.
DR eggNOG; ENOG502QR2J; Eukaryota.
DR HOGENOM; CLU_046066_0_1_1; -.
DR InParanoid; O80472; -.
DR OMA; NIVREDY; -.
DR OrthoDB; 923240at2759; -.
DR PhylomeDB; O80472; -.
DR BioCyc; ARA:AT2G23560-MON; -.
DR PRO; PR:O80472; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O80472; baseline and differential.
DR Genevisible; O80472; AT.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IDA:TAIR.
DR GO; GO:0080030; F:methyl indole-3-acetate esterase activity; IDA:TAIR.
DR GO; GO:0080032; F:methyl jasmonate esterase activity; IBA:GO_Central.
DR GO; GO:0080031; F:methyl salicylate esterase activity; IMP:TAIR.
DR GO; GO:0050832; P:defense response to fungus; TAS:TAIR.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0009694; P:jasmonic acid metabolic process; IBA:GO_Central.
DR GO; GO:0009696; P:salicylic acid metabolic process; IDA:TAIR.
DR GO; GO:0009627; P:systemic acquired resistance; IGI:TAIR.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR000073; AB_hydrolase_1.
DR InterPro; IPR045889; MES/HNL.
DR PANTHER; PTHR10992; PTHR10992; 1.
DR Pfam; PF12697; Abhydrolase_6; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 1: Evidence at protein level;
KW Hydrolase; Immunity; Innate immunity; Plant defense; Reference proteome.
FT CHAIN 1..260
FT /note="Methylesterase 7"
FT /id="PRO_0000418181"
FT ACT_SITE 84
FT /note="Acyl-ester intermediate"
FT /evidence="ECO:0000250|UniProtKB:Q6RYA0"
FT ACT_SITE 210
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:Q6RYA0"
FT ACT_SITE 238
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:Q6RYA0"
SQ SEQUENCE 260 AA; 28823 MW; A9062F20975F716E CRC64;
MDKNNQKKFV LVHGICHGAW CWYKVKAQLE AAGHSVTAVD LAASGVNMTS LDEIQTLKDY
CKPLLEFLSS LGSDDDKVIL VAHSMGGISA SLAADIFPSK VAAIVFVAAF MPDISNPPAY
VFQKLVKDVT QEVWMDTVFG KPDRPLEFAL FGPEFMAKYL YNLSPLQDFE LAKMSVRVSP
FMTNNLAGTI SFSEDRYGSV TRIYIVCGED VAVPVDYQRG MINDFPVKEV LEIKDADHMP
MFSKPQELCA LLLEIADKYA
