ID   MES8_ARATH              Reviewed;         272 AA.
AC   O80475;
DT   11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Methylesterase 8 {ECO:0000303|PubMed:18467465};
DE            Short=AtMES8 {ECO:0000303|PubMed:18467465};
DE            EC=3.1.1.- {ECO:0000269|PubMed:18467465};
GN   Name=MES8 {ECO:0000303|PubMed:18467465};
GN   OrderedLocusNames=At2g23590 {ECO:0000312|Araport:AT2G23590};
GN   ORFNames=F26B6.24 {ECO:0000312|EMBL:AAC23772.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENE FAMILY, AND FUNCTION.
RX   PubMed=18467465; DOI=10.1104/pp.108.118224;
RA   Yang Y., Xu R., Ma C.J., Vlot A.C., Klessig D.F., Pichersky E.;
RT   "Inactive methyl indole-3-acetic acid ester can be hydrolyzed and activated
RT   by several esterases belonging to the AtMES esterase family of
RT   Arabidopsis.";
RL   Plant Physiol. 147:1034-1045(2008).
CC   -!- FUNCTION: Methylesterase shown to have carboxylesterase activity in
CC       vitro. {ECO:0000269|PubMed:18467465}.
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Methylesterase
CC       family. {ECO:0000305}.
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DR   EMBL; AC003040; AAC23772.1; -; Genomic_DNA.
DR   EMBL; CP002685; AEC07470.1; -; Genomic_DNA.
DR   PIR; T01148; T01148.
DR   RefSeq; NP_179940.1; NM_127923.2.
DR   AlphaFoldDB; O80475; -.
DR   SMR; O80475; -.
DR   STRING; 3702.AT2G23590.1; -.
DR   ESTHER; arath-MES8; Hydroxynitrile_lyase.
DR   PaxDb; O80475; -.
DR   PRIDE; O80475; -.
DR   ProteomicsDB; 238962; -.
DR   EnsemblPlants; AT2G23590.1; AT2G23590.1; AT2G23590.
DR   GeneID; 816891; -.
DR   Gramene; AT2G23590.1; AT2G23590.1; AT2G23590.
DR   KEGG; ath:AT2G23590; -.
DR   Araport; AT2G23590; -.
DR   TAIR; locus:2046842; AT2G23590.
DR   eggNOG; ENOG502QR2J; Eukaryota.
DR   HOGENOM; CLU_046066_0_1_1; -.
DR   InParanoid; O80475; -.
DR   OMA; HEYTRPL; -.
DR   OrthoDB; 923240at2759; -.
DR   PhylomeDB; O80475; -.
DR   BioCyc; ARA:AT2G23590-MON; -.
DR   PRO; PR:O80475; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; O80475; baseline and differential.
DR   Genevisible; O80475; AT.
DR   GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IDA:TAIR.
DR   GO; GO:0080030; F:methyl indole-3-acetate esterase activity; IBA:GO_Central.
DR   GO; GO:0080032; F:methyl jasmonate esterase activity; IBA:GO_Central.
DR   GO; GO:0080031; F:methyl salicylate esterase activity; IBA:GO_Central.
DR   GO; GO:0009694; P:jasmonic acid metabolic process; IBA:GO_Central.
DR   GO; GO:0009696; P:salicylic acid metabolic process; IBA:GO_Central.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   InterPro; IPR045889; MES/HNL.
DR   PANTHER; PTHR10992; PTHR10992; 1.
DR   Pfam; PF12697; Abhydrolase_6; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..272
FT                   /note="Methylesterase 8"
FT                   /id="PRO_0000418182"
FT   ACT_SITE        102
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000250|UniProtKB:Q6RYA0"
FT   ACT_SITE        222
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:Q6RYA0"
FT   ACT_SITE        250
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:Q6RYA0"
SQ   SEQUENCE   272 AA;  30687 MW;  AB67C8F2B6A32C90 CRC64;
     MYENGISFII SLLICGCVKS EEMMKQHFVL VHGSCLGAWC WYKVKPLLEA SGHRVTALDL
     AACGIDTRSI TDISTCEQYS EPLIQLMTSL PNDEKVVLVG HSYGGLTLAI AMDKFPDKIS
     VSVFVTSFMP DTKNSPSFVL EKFASTMTPE DWMGSELEPY VVFSAEFTKH RILQLSPIED
     LELRLLLKRP GSLFLNDLSR MKNFSEKGYG SVPRAYIVSK DDHTISEEYQ RWMIDNYPPN
     LVIEMEGTDH LPLFCKPQLL SDHLLAIADK FS