MESD_DROME
ID MESD_DROME Reviewed; 180 AA.
AC Q8T9B6; A1Z712;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 22-SEP-2009, sequence version 2.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=LDLR chaperone boca;
DE Flags: Precursor;
GN Name=boca; ORFNames=CG30498;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, INTERACTION WITH ARROW AND YOLKLESS, SUBCELLULAR LOCATION, AND
RP MUTAGENESIS OF TRP-49.
RX PubMed=12581524; DOI=10.1016/s0092-8674(02)01279-5;
RA Culi J., Mann R.S.;
RT "Boca, an endoplasmic reticulum protein required for wingless signaling and
RT trafficking of LDL receptor family members in Drosophila.";
RL Cell 112:343-354(2003).
CC -!- FUNCTION: Chaperone specifically assisting the folding of beta-
CC propeller/EGF modules within the family of low-density lipoprotein
CC receptors (LDLRs). Acts as a modulator of the Wg pathway, since some
CC LDLRs are coreceptors for the canonical Wnt pathway.
CC {ECO:0000269|PubMed:12581524}.
CC -!- SUBUNIT: Monomer (By similarity). Interacts with Arrow and Yolkless.
CC {ECO:0000250, ECO:0000269|PubMed:12581524}.
CC -!- INTERACTION:
CC Q8T9B6; Q8T9B6: boca; NbExp=3; IntAct=EBI-15914860, EBI-15914860;
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum {ECO:0000255|PROSITE-
CC ProRule:PRU10138, ECO:0000269|PubMed:12581524}.
CC -!- DOMAIN: The LDLR maturation activity resides in the N- and C-terminal
CC unstructured regions. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the MESD family. {ECO:0000305}.
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DR EMBL; AE013599; AAF59229.2; -; Genomic_DNA.
DR EMBL; AY069840; AAL39985.1; -; mRNA.
DR EMBL; BT044294; ACH92359.1; -; mRNA.
DR RefSeq; NP_724578.1; NM_165541.2.
DR PDB; 3OFE; X-ray; 2.29 A; A/B=88-172.
DR PDB; 3OFF; X-ray; 2.00 A; A=89-172.
DR PDBsum; 3OFE; -.
DR PDBsum; 3OFF; -.
DR AlphaFoldDB; Q8T9B6; -.
DR SMR; Q8T9B6; -.
DR BioGRID; 71878; 76.
DR DIP; DIP-59112N; -.
DR ELM; Q8T9B6; -.
DR IntAct; Q8T9B6; 3.
DR STRING; 7227.FBpp0088027; -.
DR PaxDb; Q8T9B6; -.
DR PRIDE; Q8T9B6; -.
DR DNASU; 48986; -.
DR EnsemblMetazoa; FBtr0088953; FBpp0088027; FBgn0004132.
DR GeneID; 48986; -.
DR KEGG; dme:Dmel_CG30498; -.
DR UCSC; CG30498-RA; d. melanogaster.
DR CTD; 48986; -.
DR FlyBase; FBgn0004132; boca.
DR VEuPathDB; VectorBase:FBgn0004132; -.
DR eggNOG; KOG4357; Eukaryota.
DR GeneTree; ENSGT00390000000993; -.
DR HOGENOM; CLU_111621_1_0_1; -.
DR InParanoid; Q8T9B6; -.
DR OMA; RPQPKID; -.
DR OrthoDB; 1590303at2759; -.
DR PhylomeDB; Q8T9B6; -.
DR BioGRID-ORCS; 48986; 0 hits in 1 CRISPR screen.
DR EvolutionaryTrace; Q8T9B6; -.
DR GenomeRNAi; 48986; -.
DR PRO; PR:Q8T9B6; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0004132; Expressed in eye disc (Drosophila) and 22 other tissues.
DR Genevisible; Q8T9B6; DM.
DR GO; GO:0045177; C:apical part of cell; IDA:FlyBase.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0012505; C:endomembrane system; HDA:FlyBase.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:FlyBase.
DR GO; GO:0005788; C:endoplasmic reticulum lumen; IDA:FlyBase.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IEP:FlyBase.
DR GO; GO:0006457; P:protein folding; IMP:FlyBase.
DR GO; GO:0006612; P:protein targeting to membrane; IMP:FlyBase.
DR GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR DisProt; DP02312; -.
DR InterPro; IPR019330; MESD.
DR PANTHER; PTHR17600; PTHR17600; 1.
DR Pfam; PF10185; Mesd; 1.
DR PROSITE; PS00014; ER_TARGET; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chaperone; Endoplasmic reticulum; Reference proteome; Signal;
KW Wnt signaling pathway.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..180
FT /note="LDLR chaperone boca"
FT /id="PRO_0000385021"
FT REGION 48..78
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 93..166
FT /note="Structured core"
FT /evidence="ECO:0000250"
FT MOTIF 177..180
FT /note="Prevents secretion from ER"
FT MUTAGEN 49
FT /note="W->R: In boca1; induces lethality."
FT /evidence="ECO:0000269|PubMed:12581524"
FT CONFLICT 15
FT /note="L -> V (in Ref. 3; AAL39985)"
FT /evidence="ECO:0000305"
FT STRAND 94..103
FT /evidence="ECO:0007829|PDB:3OFF"
FT HELIX 106..122
FT /evidence="ECO:0007829|PDB:3OFF"
FT STRAND 127..133
FT /evidence="ECO:0007829|PDB:3OFF"
FT STRAND 136..143
FT /evidence="ECO:0007829|PDB:3OFF"
FT HELIX 144..146
FT /evidence="ECO:0007829|PDB:3OFF"
FT HELIX 147..154
FT /evidence="ECO:0007829|PDB:3OFF"
FT STRAND 160..165
FT /evidence="ECO:0007829|PDB:3OFF"
FT STRAND 168..171
FT /evidence="ECO:0007829|PDB:3OFF"
SQ SEQUENCE 180 AA; 21002 MW; B51E057C901A6546 CRC64;
MQTRLVLLLL ALTPLVLAKK FKEEEKPAWA KKDIRDYSEA DLERLLDQWE EDEEPLEDDE
LPEHLRPQPK LDLSNLDSKS PEDLLKVSKK GRTLMTFVSV TGNPTREESD TITKLWQTSL
WNNHIQAERY MVDDNRAIFL FKDGTQAWDA KDFLIEQERC KGVTIENKEY PGVNAKKDEL
