MESH1_DANRE
ID MESH1_DANRE Reviewed; 180 AA.
AC Q568P1;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1;
DE EC=3.1.7.2;
DE AltName: Full=HD domain-containing protein 3;
DE AltName: Full=Metazoan SpoT homolog 1;
DE Short=MESH1;
DE AltName: Full=Penta-phosphate guanosine-3'-pyrophosphohydrolase;
DE Short=(ppGpp)ase;
GN Name=hddc3; Synonyms=Mesh1; ORFNames=zgc:110184;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: ppGpp hydrolyzing enzyme involved in starvation response.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=guanosine 3',5'-bis(diphosphate) + H2O = diphosphate + GDP +
CC H(+); Xref=Rhea:RHEA:14253, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:58189, ChEBI:CHEBI:77828; EC=3.1.7.2;
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC -!- SIMILARITY: Belongs to the MESH1 family. {ECO:0000305}.
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DR EMBL; BC092780; AAH92780.1; -; mRNA.
DR RefSeq; NP_001017835.1; NM_001017835.1.
DR AlphaFoldDB; Q568P1; -.
DR SMR; Q568P1; -.
DR STRING; 7955.ENSDARP00000033279; -.
DR PaxDb; Q568P1; -.
DR GeneID; 550533; -.
DR KEGG; dre:550533; -.
DR CTD; 374659; -.
DR ZFIN; ZDB-GENE-050417-377; hddc3.
DR eggNOG; KOG1157; Eukaryota.
DR InParanoid; Q568P1; -.
DR OrthoDB; 1372331at2759; -.
DR PhylomeDB; Q568P1; -.
DR PRO; PR:Q568P1; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Unplaced.
DR GO; GO:0008893; F:guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd00077; HDc; 1.
DR InterPro; IPR003607; HD/PDEase_dom.
DR InterPro; IPR006674; HD_domain.
DR SMART; SM00471; HDc; 1.
DR PROSITE; PS51831; HD; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Manganese; Metal-binding; Reference proteome.
FT CHAIN 1..180
FT /note="Guanosine-3',5'-bis(diphosphate) 3'-
FT pyrophosphohydrolase MESH1"
FT /id="PRO_0000263112"
FT DOMAIN 33..128
FT /note="HD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01175"
FT ACT_SITE 66
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 67
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT BINDING 36
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 62
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 63
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 123
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
SQ SEQUENCE 180 AA; 20296 MW; 6CC9689FCDE3E8DE CRC64;
MNCSSTAVLL ETVNFAAEKH RNQRHKDPEG TPYINHPIGV ARILSHEGEI TDVEVIQAAL
LHDTVEDTDT TFEELESVFG ATVARIVQGV TDDKSLPKAE RERQQVEHAP HCSHQAKLVK
LADKLYNLRD LNRCTPEGWS AQRVQEYFEW ASQVVKGLRG TNAAIEEKLQ QLFLERGVKL
