ARIR_ECOLI
ID ARIR_ECOLI Reviewed; 88 AA.
AC P75993; Q2MBG9;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Probable two-component-system connector protein AriR;
GN Name=ariR; Synonyms=ymgB; OrderedLocusNames=b1166, JW1153;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP REPRESSION BY AI-2.
RC STRAIN=K12;
RX PubMed=15470704; DOI=10.1002/bit.20259;
RA Ren D., Bedzyk L.A., Ye R.W., Thomas S.M., Wood T.K.;
RT "Differential gene expression shows natural brominated furanones interfere
RT with the autoinducer-2 bacterial signaling system of Escherichia coli.";
RL Biotechnol. Bioeng. 88:630-642(2004).
RN [4]
RP REPRESSION BY INDOLE.
RC STRAIN=K12;
RX PubMed=17511876; DOI=10.1186/1471-2180-7-42;
RA Lee J., Jayaraman A., Wood T.K.;
RT "Indole is an inter-species biofilm signal mediated by SdiA.";
RL BMC Microbiol. 7:42-42(2007).
RN [5]
RP INDUCTION BY LOW TEMPERATURE, AND RPOS-INDEPENDENCE.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=18174134; DOI=10.1099/mic.0.2007/012021-0;
RA White-Ziegler C.A., Um S., Perez N.M., Berns A.L., Malhowski A.J.,
RA Young S.;
RT "Low temperature (23 degrees C) increases expression of biofilm-, cold-
RT shock- and RpoS-dependent genes in Escherichia coli K-12.";
RL Microbiology 154:148-166(2008).
RN [6]
RP INDUCTION BY 5-FLUOROURACIL.
RC STRAIN=K12 / ATCC 25404 / DSM 5698 / NCIMB 11290;
RX PubMed=19172264; DOI=10.1007/s00253-009-1860-8;
RA Attila C., Ueda A., Wood T.K.;
RT "5-Fluorouracil reduces biofilm formation in Escherichia coli K-12 through
RT global regulator AriR as an antivirulence compound.";
RL Appl. Microbiol. Biotechnol. 82:525-533(2009).
RN [7]
RP INDUCTION BY COLD, RPOS-DEPENDENCE, REPRESSION BY YCGE, AND OPERON
RP STRUCTURE.
RC STRAIN=K12 / MC4100;
RX PubMed=19240136; DOI=10.1101/gad.499409;
RA Tschowri N., Busse S., Hengge R.;
RT "The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light
RT response of Escherichia coli.";
RL Genes Dev. 23:522-534(2009).
RN [8]
RP X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 25-88, FUNCTION, DNA-BINDING,
RP SUBUNIT, AND DISRUPTION PHENOTYPE.
RC STRAIN=K12;
RX PubMed=17765265; DOI=10.1016/j.jmb.2007.07.037;
RA Lee J., Page R., Garcia-Contreras R., Palermino J.-M., Zhang X.-S.,
RA Doshi O., Wood T.K., Peti W.;
RT "Structure and function of the Escherichia coli protein YmgB: a protein
RT critical for biofilm formation and acid-resistance.";
RL J. Mol. Biol. 373:11-26(2007).
CC -!- FUNCTION: Probably a connector protein for RcsB/C regulation of biofilm
CC and acid-resistance, providing additional signal input into the two-
CC component signaling pathway. May serve to stimulate biofilm maturation,
CC via the Rcs phosphorelay. Regulates expression of genes involved in
CC acid-resistance and biofilm formation, including the RcsB/C two-
CC component system. May be a non-specific DNA-binding protein that binds
CC genes and/or intergenic regions via a geometric recognition. Also
CC confers resistance to H(2)O(2). Overexpression at 28 and 16 degrees
CC Celsius increases the production of colanic acid, an exopolysaccharide
CC and matrix component, and reduces adhesive curli fimbriae expression.
CC Both of these effects require RcsB; AriR probably acts upstream of the
CC RcsB/C system to stimulate the activity and not transcription of
CC RcsB/C. 5-fluorouracil reduction in biofilm formation requires this
CC protein. {ECO:0000269|PubMed:17765265}.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:17765265}.
CC -!- INDUCTION: Induced by low temperature (23 degrees Celsius). Repressed
CC by the autoinducer-2 (AI-2) and by indole. Induced by 5-fluorouracil.
CC Strongly induced at 16 degrees Celsius. Expression has been shown to be
CC independent of RpoS (PubMed:18174134) and dependent on RpoS
CC (PubMed:19240136), repressed by YcgE. At 16 degrees Celsius with blue
CC light irradiation expression of this operon is absolutely dependent on
CC YcgF for relief of YcgE repression. Part of the ycgZ-ymgA-ariR-ymgC
CC operon, it probably also has its own promoter.
CC {ECO:0000269|PubMed:15470704, ECO:0000269|PubMed:17511876,
CC ECO:0000269|PubMed:18174134, ECO:0000269|PubMed:19172264,
CC ECO:0000269|PubMed:19240136}.
CC -!- DISRUPTION PHENOTYPE: Deletion increases biofilm formation.
CC {ECO:0000269|PubMed:17765265}.
CC -!- SIMILARITY: Belongs to the AriR family. {ECO:0000305}.
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DR EMBL; U00096; AAC74250.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76387.1; -; Genomic_DNA.
DR PIR; C64862; C64862.
DR RefSeq; NP_415684.1; NC_000913.3.
DR RefSeq; WP_000888772.1; NZ_SSZK01000010.1.
DR PDB; 2OXL; X-ray; 1.80 A; A/B=25-88.
DR PDBsum; 2OXL; -.
DR AlphaFoldDB; P75993; -.
DR SMR; P75993; -.
DR BioGRID; 4263105; 9.
DR BioGRID; 849716; 1.
DR IntAct; P75993; 1.
DR STRING; 511145.b1166; -.
DR jPOST; P75993; -.
DR PaxDb; P75993; -.
DR PRIDE; P75993; -.
DR EnsemblBacteria; AAC74250; AAC74250; b1166.
DR EnsemblBacteria; BAE76387; BAE76387; BAE76387.
DR GeneID; 66675018; -.
DR GeneID; 945340; -.
DR KEGG; ecj:JW1153; -.
DR KEGG; eco:b1166; -.
DR PATRIC; fig|1411691.4.peg.1125; -.
DR EchoBASE; EB4038; -.
DR eggNOG; ENOG50336K1; Bacteria.
DR HOGENOM; CLU_164045_1_0_6; -.
DR OMA; EMVVGMT; -.
DR PhylomeDB; P75993; -.
DR BioCyc; EcoCyc:G6606-MON; -.
DR EvolutionaryTrace; P75993; -.
DR PRO; PR:P75993; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003677; F:DNA binding; IDA:EcoCyc.
DR GO; GO:0071468; P:cellular response to acidic pH; IEA:InterPro.
DR GO; GO:0042542; P:response to hydrogen peroxide; IMP:EcoCyc.
DR GO; GO:0044010; P:single-species biofilm formation; IMP:EcoCyc.
DR InterPro; IPR024753; AriR.
DR Pfam; PF10798; YmgB; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Reference proteome.
FT CHAIN 1..88
FT /note="Probable two-component-system connector protein
FT AriR"
FT /id="PRO_0000168863"
FT HELIX 28..43
FT /evidence="ECO:0007829|PDB:2OXL"
FT HELIX 50..62
FT /evidence="ECO:0007829|PDB:2OXL"
FT HELIX 67..85
FT /evidence="ECO:0007829|PDB:2OXL"
SQ SEQUENCE 88 AA; 9694 MW; 0A67FC4289CCD46F CRC64;
MLEDTTIHNA ITDKALASYF RSSGNLLEEE SAVLGQAVTN LMLSGDNVNN KNIILSLIHS
LETTSDILKA DVIRKTLEIV LRYTADDM
