MESP1_MOUSE
ID MESP1_MOUSE Reviewed; 243 AA.
AC P97309; Q059W2;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Mesoderm posterior protein 1;
GN Name=Mesp1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=ICR;
RX PubMed=8787751; DOI=10.1242/dev.122.9.2769;
RA Saga Y., Hata N., Kobayashi S., Magnuson T., Seldin M.F., Taketo M.M.;
RT "MesP1: a novel basic helix-loop-helix protein expressed in the nascent
RT mesodermal cells during mouse gastrulation.";
RL Development 122:2769-2778(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=10887078; DOI=10.1242/dev.127.15.3215;
RA Kitajima S., Takagi A., Inoue T., Saga Y.;
RT "MesP1 and MesP2 are essential for the development of cardiac mesoderm.";
RL Development 127:3215-3226(2000).
RN [4]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=15677726; DOI=10.1242/dev.01597;
RA Takahashi Y., Kitajima S., Inoue T., Kanno J., Saga Y.;
RT "Differential contributions of Mesp1 and Mesp2 to the epithelialization and
RT rostro-caudal patterning of somites.";
RL Development 132:787-796(2005).
RN [5]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=17306789; DOI=10.1016/j.ydbio.2007.01.007;
RA Takahashi Y., Yasuhiko Y., Kitajima S., Kanno J., Saga Y.;
RT "Appropriate suppression of Notch signaling by Mesp factors is essential
RT for stripe pattern formation leading to segment boundary formation.";
RL Dev. Biol. 304:593-603(2007).
CC -!- FUNCTION: Transcription factor. Plays a role in the epithelialization
CC of somitic mesoderm and in the development of cardiac mesoderm. Defines
CC the rostrocaudal patterning of the somites by participating in distinct
CC Notch pathways. {ECO:0000269|PubMed:15677726,
CC ECO:0000269|PubMed:17306789}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: No expression was detected in adult tissues except
CC the testis. Expression in the testis was regulated developmentally;
CC expressed 2 weeks after birth, and increases, reaching the full
CC expression level in mature testes. {ECO:0000269|PubMed:8787751}.
CC -!- DEVELOPMENTAL STAGE: The earliest expression is detected at the
CC junction of the epiblast and extraembryonic ectoderm, which is the
CC initiation site for gastrulation. Around 6.5-6.75 dpc, expression
CC becomes evident as gastrulation progresses. Expression does not spread
CC over all mesodermal cells; it is seen only in a fraction of cells,
CC particularly those in the early emergence group destined to become the
CC extraembryonic mesoderm and the most posterior part of the tailbud.
CC Expression remains for a while at the base of the allantois, and then
CC spreads out to the lateral margins of the tail bud mesoderm. Expressed
CC in the anterior presomitic mesoderm in a broad or thin stripe pattern.
CC Expressed in the paraxial mesoderm during somitogenesis. Expression
CC disappears before 8.5 dpc. {ECO:0000269|PubMed:10887078,
CC ECO:0000269|PubMed:15677726, ECO:0000269|PubMed:17306789,
CC ECO:0000269|PubMed:8787751}.
CC -!- INDUCTION: Expression is induced by Notch signaling.
CC -!- DISRUPTION PHENOTYPE: Mice embryos exhibit defects in single heart tube
CC formation, due to a delay in mesodermal migration. However, the cells
CC eventually acquired migratory activity and gave rise to an abnormal
CC heart tube. Mice lacking Mesp1 and Mesp2 die around 9.5 dpc. The major
CC defect is the apparent lack of any mesodermal layer between endoderm
CC and ectoderm and a defect in the migratory activity of mesodermal
CC cells. It seems that a compensatory mechanism exists in which Mesp1
CC expression is up-regulated in the absence of Mesp2. Mesp1 may be
CC involved in the rescue of somitogenesis in the null Mesp2 embryos.
CC {ECO:0000269|PubMed:10887078, ECO:0000269|PubMed:15677726,
CC ECO:0000269|PubMed:17306789}.
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DR EMBL; D83674; BAA12041.1; -; mRNA.
DR EMBL; BC125505; AAI25506.1; -; mRNA.
DR EMBL; BC125507; AAI25508.1; -; mRNA.
DR CCDS; CCDS21387.1; -.
DR RefSeq; NP_032614.2; NM_008588.2.
DR AlphaFoldDB; P97309; -.
DR SMR; P97309; -.
DR STRING; 10090.ENSMUSP00000032760; -.
DR PhosphoSitePlus; P97309; -.
DR PaxDb; P97309; -.
DR PRIDE; P97309; -.
DR Antibodypedia; 28662; 328 antibodies from 31 providers.
DR DNASU; 17292; -.
DR Ensembl; ENSMUST00000032760; ENSMUSP00000032760; ENSMUSG00000030544.
DR GeneID; 17292; -.
DR KEGG; mmu:17292; -.
DR UCSC; uc009hzb.2; mouse.
DR CTD; 55897; -.
DR MGI; MGI:107785; Mesp1.
DR VEuPathDB; HostDB:ENSMUSG00000030544; -.
DR eggNOG; KOG4029; Eukaryota.
DR GeneTree; ENSGT00530000063712; -.
DR HOGENOM; CLU_064749_1_0_1; -.
DR InParanoid; P97309; -.
DR OMA; PLSESWM; -.
DR OrthoDB; 1072866at2759; -.
DR PhylomeDB; P97309; -.
DR TreeFam; TF325707; -.
DR BioGRID-ORCS; 17292; 1 hit in 73 CRISPR screens.
DR ChiTaRS; Mesp1; mouse.
DR PRO; PR:P97309; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; P97309; protein.
DR Bgee; ENSMUSG00000030544; Expressed in seminiferous tubule of testis and 45 other tissues.
DR Genevisible; P97309; MM.
DR GO; GO:0005634; C:nucleus; IDA:BHF-UCL.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IDA:BHF-UCL.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IMP:BHF-UCL.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; ISO:MGI.
DR GO; GO:0003210; P:cardiac atrium formation; ISO:MGI.
DR GO; GO:0060913; P:cardiac cell fate determination; IDA:BHF-UCL.
DR GO; GO:0055007; P:cardiac muscle cell differentiation; ISO:MGI.
DR GO; GO:0060947; P:cardiac vascular smooth muscle cell differentiation; ISO:MGI.
DR GO; GO:0003211; P:cardiac ventricle formation; ISO:MGI.
DR GO; GO:0003259; P:cardioblast anterior-lateral migration; IEP:BHF-UCL.
DR GO; GO:0003260; P:cardioblast migration; IMP:MGI.
DR GO; GO:0060975; P:cardioblast migration to the midline involved in heart field formation; IMP:BHF-UCL.
DR GO; GO:0003143; P:embryonic heart tube morphogenesis; IMP:BHF-UCL.
DR GO; GO:0045446; P:endothelial cell differentiation; ISO:MGI.
DR GO; GO:0007369; P:gastrulation; IEP:BHF-UCL.
DR GO; GO:0010467; P:gene expression; IMP:MGI.
DR GO; GO:0003241; P:growth involved in heart morphogenesis; IMP:BHF-UCL.
DR GO; GO:0001947; P:heart looping; IMP:BHF-UCL.
DR GO; GO:0003007; P:heart morphogenesis; IMP:MGI.
DR GO; GO:0048368; P:lateral mesoderm development; IEP:BHF-UCL.
DR GO; GO:0001707; P:mesoderm formation; IBA:GO_Central.
DR GO; GO:0008078; P:mesodermal cell migration; IGI:MGI.
DR GO; GO:0042664; P:negative regulation of endodermal cell fate specification; IDA:BHF-UCL.
DR GO; GO:0042662; P:negative regulation of mesodermal cell fate specification; IDA:BHF-UCL.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:BHF-UCL.
DR GO; GO:0022008; P:neurogenesis; ISO:MGI.
DR GO; GO:0007219; P:Notch signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0090082; P:positive regulation of heart induction by negative regulation of canonical Wnt signaling pathway; IDA:BHF-UCL.
DR GO; GO:0070368; P:positive regulation of hepatocyte differentiation; IDA:BHF-UCL.
DR GO; GO:0045747; P:positive regulation of Notch signaling pathway; IDA:BHF-UCL.
DR GO; GO:0035481; P:positive regulation of Notch signaling pathway involved in heart induction; IDA:BHF-UCL.
DR GO; GO:0051155; P:positive regulation of striated muscle cell differentiation; IDA:BHF-UCL.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:BHF-UCL.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:BHF-UCL.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0003139; P:secondary heart field specification; IDA:BHF-UCL.
DR GO; GO:0023019; P:signal transduction involved in regulation of gene expression; IDA:MGI.
DR GO; GO:0060921; P:sinoatrial node cell differentiation; ISO:MGI.
DR GO; GO:0003236; P:sinus venosus morphogenesis; IMP:BHF-UCL.
DR GO; GO:0032525; P:somite rostral/caudal axis specification; IBA:GO_Central.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR040259; Mesogenin/MesP.
DR PANTHER; PTHR20937; PTHR20937; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Notch signaling pathway; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation.
FT CHAIN 1..243
FT /note="Mesoderm posterior protein 1"
FT /id="PRO_0000304405"
FT DOMAIN 76..130
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..86
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 204..228
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 153..157
FT /note="CPLCP"
FT COMPBIAS 26..44
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 204..226
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 170
FT /note="R -> G (in Ref. 1; BAA12041)"
FT /evidence="ECO:0000305"
FT CONFLICT 202
FT /note="L -> Q (in Ref. 1; BAA12041)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 243 AA; 26386 MW; D82F72C0B0FB05EE CRC64;
MAQPLCEPRS ESWILSPAGR QPPMPSDGNS VCSPAWSSDP WDGAQASSPA PPCARPARRA
GTPGRRGTHG SRLGSGQRQS ASEREKLRMR TLARALHELR RFLPPSVAPT GQNLTKIETL
RLAIRYIGHL SAVLGLSEDN LRRQRHAVSP RGCPLCPDSD LAQSQSLGPR LSPAVCSGVS
WGSPPAYPRP RVAAESWDPS FLYAETASQE RQEMEPSPSS PLFSSDMLAL LETWTPPQEW
PPA
