ID   MEST_BOVIN              Reviewed;         335 AA.
AC   Q2HJM9; A3KN56; Q6U8D3; Q6U8D4;
DT   17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   17-APR-2007, sequence version 2.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Mesoderm-specific transcript homolog protein;
DE            EC=3.-.-.-;
DE   AltName: Full=Paternally-expressed gene 1 protein;
GN   Name=MEST; Synonyms=PEG1;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   STRAIN=Hereford; TISSUE=Fetal spinal cord;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 3-335 (ISOFORM 1).
RA   Yang L., Curchoe C., Tian X.C.;
RT   "Peg1.";
RL   Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 10-253 (ISOFORMS 1 AND 2), ALTERNATIVE
RP   SPLICING, AND TISSUE SPECIFICITY.
RX   PubMed=14668210; DOI=10.1095/biolreprod.103.022236;
RA   Ruddock N.T., Wilson K.J., Cooney M.A., Korfiatis N.A., Tecirlioglu R.T.,
RA   French A.J.;
RT   "Analysis of imprinted messenger RNA expression during bovine
RT   preimplantation development.";
RL   Biol. Reprod. 70:1131-1135(2004).
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Multi-pass membrane protein {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q2HJM9-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q2HJM9-2; Sequence=VSP_024535;
CC   -!- TISSUE SPECIFICITY: No detectable transcripts during preimplantation
CC       development. Isoform 1 was not detected in either in vitro-matured
CC       oocytes (IVF) or parthenogenetically activated (PA) blastocyst. Isoform
CC       2 was expressed in IVF and PA blastocysts.
CC       {ECO:0000269|PubMed:14668210}.
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. {ECO:0000305}.
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DR   EMBL; BC133639; AAI33640.1; -; mRNA.
DR   EMBL; AY957983; ABD34311.1; -; mRNA.
DR   EMBL; AY376068; AAR04853.1; -; mRNA.
DR   EMBL; AY376069; AAR04854.1; -; mRNA.
DR   RefSeq; NP_001076837.1; NM_001083368.1. [Q2HJM9-1]
DR   AlphaFoldDB; Q2HJM9; -.
DR   SMR; Q2HJM9; -.
DR   STRING; 9913.ENSBTAP00000042286; -.
DR   ESTHER; bovin-q2hjm9; MEST-like.
DR   PaxDb; Q2HJM9; -.
DR   GeneID; 404180; -.
DR   KEGG; bta:404180; -.
DR   CTD; 4232; -.
DR   eggNOG; KOG4178; Eukaryota.
DR   InParanoid; Q2HJM9; -.
DR   OrthoDB; 1268438at2759; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   InterPro; IPR000639; Epox_hydrolase-like.
DR   Pfam; PF00561; Abhydrolase_1; 1.
DR   PRINTS; PR00412; EPOXHYDRLASE.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Endoplasmic reticulum; Hydrolase; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..335
FT                   /note="Mesoderm-specific transcript homolog protein"
FT                   /id="PRO_0000284417"
FT   TRANSMEM        13..33
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        88..108
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        266..286
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          71..310
FT                   /note="AB hydrolase-1"
FT                   /evidence="ECO:0000255"
FT   MOTIF           98..103
FT                   /note="RVIALD"
FT   VAR_SEQ         1..9
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:14668210"
FT                   /id="VSP_024535"
FT   CONFLICT        95
FT                   /note="S -> R (in Ref. 2; ABD34311)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        214
FT                   /note="S -> P (in Ref. 3; AAR04854)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        240
FT                   /note="R -> P (in Ref. 3; AAR04854)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   335 AA;  38724 MW;  1F44695A3E1AB319 CRC64;
     MVRRDRLRRM REWWVQVGLL AVPLLAAYLH IPPPQLSPAL HSWKSSGKFF TYKGLRIFYQ
     DSVGVVGSPE IVVLLHGFPT SSYDWYKIWE GLTLSFHRVI ALDFLGFGFS DKPRPHHYSI
     FEQASIVEAL LRHLGLQSRR INLLSHDYGD TVAQELLYRF KQNRSGRLTI KSLCLSNGGI
     FPETHRPLLL QKLLKDGGML SPILTRLMNF FVFSRGLTPV FGPYTRPSES ELWDMWAGIR
     NNDGNLVIDS LLQYINQRKK FRRRWVGALA SVSIPIHFIY GPLDPVNPYP EFLELYRKTL
     PRSTVSILDD HISHYPQLED PMGFLNAYMG FINSF