MEST_MOUSE
ID MEST_MOUSE Reviewed; 335 AA.
AC Q07646; Q792T8; Q8BS88; Q8K463; Q99KT5;
DT 17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Mesoderm-specific transcript protein;
DE EC=3.-.-.-;
DE AltName: Full=Paternally-expressed gene 1 protein;
GN Name=Mest; Synonyms=Peg1; ORFNames=121a;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND DEVELOPMENTAL STAGE.
RA Sado T., Nakajima N., Tada M., Takagi N.;
RT "A novel mesoderm-specific cDNA isolated from a mouse embryonal carcinoma
RT cell line.";
RL Dev. Growth Differ. 35:551-560(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND ALTERNATIVE SPLICING.
RC STRAIN=C57BL/6J;
RX PubMed=12095916; DOI=10.1093/hmg/11.15.1743;
RA Nakabayashi K., Bentley L., Hitchins M.P., Mitsuya K., Meguro M.,
RA Minagawa S., Bamforth J.S., Stanier P., Preece M., Weksberg R.,
RA Oshimura M., Moore G.E., Scherer S.W.;
RT "Identification and characterization of an imprinted antisense RNA
RT (MESTIT1) in the human MEST locus on chromosome 7q32.";
RL Hum. Mol. Genet. 11:1743-1756(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Amnion, Embryo, Heart, Kidney, and Wolffian duct;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=Czech II; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-60.
RC STRAIN=129/Sv;
RX PubMed=9302270; DOI=10.1093/hmg/6.11.1907;
RA Lefebvre L., Viville S., Barton S.C., Ishino F., Surani M.A.;
RT "Genomic structure and parent-of-origin-specific methylation of Peg1.";
RL Hum. Mol. Genet. 6:1907-1915(1997).
RN [6]
RP IDENTIFICATION, AND TISSUE SPECIFICITY.
RC STRAIN=129/Sv;
RX PubMed=7550314; DOI=10.1038/ng0995-52;
RA Kaneko-Ishino T., Kuroiwa Y., Miyoshi N., Kohda T., Suzuki R., Yokoyama M.,
RA Viville S., Barton S.C., Ishino F., Surani M.A.;
RT "Peg1/Mest imprinted gene on chromosome 6 identified by cDNA subtraction
RT hybridization.";
RL Nat. Genet. 11:52-59(1995).
RN [7]
RP SUBCELLULAR LOCATION.
RX PubMed=18644838; DOI=10.1096/fj.08-108266;
RA Nikonova L., Koza R.A., Mendoza T., Chao P.-M., Curley J.P., Kozak L.P.;
RT "Mesoderm-specific transcript is associated with fat mass expansion in
RT response to a positive energy balance.";
RL FASEB J. 22:3925-3937(2008).
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:18644838}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:18644838}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q07646-1; Sequence=Displayed;
CC Name=2; Synonyms=MEST intronic transcript 1;
CC IsoId=Q07646-2; Sequence=VSP_024534;
CC -!- TISSUE SPECIFICITY: Expressed in mesodermal tissues. Isoform 1 is
CC exclusively expressed from the paternal allele in all fetal tissues and
CC cell lines examined, whereas isoform 2 is preferentially expressed from
CC the paternal allele in a tissue-type-specific manner.
CC {ECO:0000269|PubMed:7550314}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the nascent mesoderm of gastrulating
CC embryos. At 7.0 dpc expression detected in both the intraembryonic
CC mesoderm and the extraembryonic mesoderm cells of the amniotic fold. At
CC 8.5 dpc expressed predominantly in the head mesenchyme, allantois, and
CC the mesodermal layer of the amnion, chorion and yolk. In 9.5 dpc
CC embryos highly expressed in the mesenchymal tissues, presomitic
CC paraxial mesoderm, heart and branchial arches. {ECO:0000269|Ref.1}.
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily. {ECO:0000305}.
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DR EMBL; D16262; BAA03795.1; -; mRNA.
DR EMBL; AF482999; AAM78507.1; -; mRNA.
DR EMBL; AK032881; BAC28068.1; -; mRNA.
DR EMBL; AK034949; BAC28891.1; -; mRNA.
DR EMBL; AK168714; BAE40556.1; -; mRNA.
DR EMBL; AK168732; BAE40573.1; -; mRNA.
DR EMBL; AK168743; BAE40584.1; -; mRNA.
DR EMBL; AK168995; BAE40794.1; -; mRNA.
DR EMBL; AK169095; BAE40878.1; -; mRNA.
DR EMBL; AK169266; BAE41027.1; -; mRNA.
DR EMBL; BC004019; AAH04019.1; -; mRNA.
DR EMBL; BC006639; AAH06639.1; -; mRNA.
DR EMBL; AF017994; AAC53396.1; -; Genomic_DNA.
DR CCDS; CCDS19979.1; -. [Q07646-1]
DR CCDS; CCDS80513.1; -. [Q07646-2]
DR RefSeq; NP_001239221.1; NM_001252292.1.
DR RefSeq; NP_001239222.1; NM_001252293.1. [Q07646-2]
DR RefSeq; NP_032616.1; NM_008590.2. [Q07646-1]
DR AlphaFoldDB; Q07646; -.
DR SMR; Q07646; -.
DR BioGRID; 201402; 1.
DR IntAct; Q07646; 1.
DR MINT; Q07646; -.
DR STRING; 10090.ENSMUSP00000129639; -.
DR ESTHER; mouse-MEST; MEST-like.
DR MEROPS; S33.972; -.
DR GlyGen; Q07646; 1 site.
DR PhosphoSitePlus; Q07646; -.
DR MaxQB; Q07646; -.
DR PaxDb; Q07646; -.
DR PeptideAtlas; Q07646; -.
DR PRIDE; Q07646; -.
DR ProteomicsDB; 295931; -. [Q07646-1]
DR ProteomicsDB; 295932; -. [Q07646-2]
DR Antibodypedia; 946; 125 antibodies from 32 providers.
DR DNASU; 17294; -.
DR Ensembl; ENSMUST00000157040; ENSMUSP00000119038; ENSMUSG00000051855. [Q07646-2]
DR Ensembl; ENSMUST00000163949; ENSMUSP00000129639; ENSMUSG00000051855. [Q07646-1]
DR GeneID; 17294; -.
DR KEGG; mmu:17294; -.
DR UCSC; uc009bfs.2; mouse. [Q07646-1]
DR CTD; 4232; -.
DR MGI; MGI:96968; Mest.
DR VEuPathDB; HostDB:ENSMUSG00000051855; -.
DR eggNOG; KOG4178; Eukaryota.
DR GeneTree; ENSGT00510000047602; -.
DR InParanoid; Q07646; -.
DR OrthoDB; 1268438at2759; -.
DR PhylomeDB; Q07646; -.
DR TreeFam; TF329307; -.
DR BioGRID-ORCS; 17294; 2 hits in 75 CRISPR screens.
DR ChiTaRS; Mest; mouse.
DR PRO; PR:Q07646; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q07646; protein.
DR Bgee; ENSMUSG00000051855; Expressed in humerus cartilage element and 280 other tissues.
DR ExpressionAtlas; Q07646; baseline and differential.
DR Genevisible; Q07646; MM.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:UniProtKB.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0010883; P:regulation of lipid storage; IMP:UniProtKB.
DR GO; GO:0032526; P:response to retinoic acid; IDA:BHF-UCL.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR000073; AB_hydrolase_1.
DR InterPro; IPR000639; Epox_hydrolase-like.
DR Pfam; PF00561; Abhydrolase_1; 1.
DR PRINTS; PR00412; EPOXHYDRLASE.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Endoplasmic reticulum; Glycoprotein; Hydrolase;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..335
FT /note="Mesoderm-specific transcript protein"
FT /id="PRO_0000284419"
FT TRANSMEM 13..33
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 63..83
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 266..286
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 71..310
FT /note="AB hydrolase-1"
FT /evidence="ECO:0000255"
FT MOTIF 98..103
FT /note="RVIALD"
FT CARBOHYD 163
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..9
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12095916"
FT /id="VSP_024534"
FT CONFLICT 43
FT /note="W -> C (in Ref. 3; BAC28891)"
FT /evidence="ECO:0000305"
FT CONFLICT 171
FT /note="K -> N (in Ref. 2; AAM78507)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 335 AA; 38907 MW; F5F394F46152E712 CRC64;
MVRRDRLRRM REWWVQVGLL AVPLLAAYLH IPPPQLSPAL HSWKTSGKFF TYKGLRIFYQ
DSVGVVGSPE IVVLLHGFPT SSYDWYKIWE GLTLRFHRVI ALDFLGFGFS DKPRPHQYSI
FEQASIVESL LRHLGLQNRR INLLSHDYGD IVAQELLYRY KQNRSGRLTI KSLCLSNGGI
FPETHRPLLL QKLLKDGGVL SPILTRLMNF FVFSRGLTPV FGPYTRPTES ELWDMWAVIR
NNDGNLVIDS LLQYINQRKK FRRRWVGALA SVSIPIHFIY GPLDPINPYP EFLELYRKTL
PRSTVSILDD HISHYPQLED PMGFLNAYMG FINSF
