MET16_DICDI
ID MET16_DICDI Reviewed; 568 AA.
AC Q554C9; Q86I21;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase;
DE EC=2.1.1.346 {ECO:0000250|UniProtKB:O42662, ECO:0000250|UniProtKB:Q86W50};
GN ORFNames=DDB_G0275203;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: RNA N6-methyltransferase that mediates N6-methylation of
CC adenine of U6 small nuclear RNA (U6 snRNA).
CC {ECO:0000250|UniProtKB:O42662, ECO:0000250|UniProtKB:Q86W50}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=adenosine in U6 snRNA + S-adenosyl-L-methionine = H(+) + N(6)-
CC methyladenosine in U6 snRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:52808, Rhea:RHEA-COMP:13573, Rhea:RHEA-COMP:13574,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:74411, ChEBI:CHEBI:74449; EC=2.1.1.346;
CC Evidence={ECO:0000250|UniProtKB:O42662,
CC ECO:0000250|UniProtKB:Q86W50};
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. METTL16/RlmF
CC family. {ECO:0000305}.
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DR EMBL; AAFI02000013; EAL69882.1; -; Genomic_DNA.
DR RefSeq; XP_643782.1; XM_638690.1.
DR AlphaFoldDB; Q554C9; -.
DR SMR; Q554C9; -.
DR STRING; 44689.DDB0203108; -.
DR PaxDb; Q554C9; -.
DR EnsemblProtists; EAL69882; EAL69882; DDB_G0275203.
DR GeneID; 8619827; -.
DR KEGG; ddi:DDB_G0275203; -.
DR dictyBase; DDB_G0275203; -.
DR eggNOG; KOG2912; Eukaryota.
DR HOGENOM; CLU_480155_0_0_1; -.
DR InParanoid; Q554C9; -.
DR OMA; CNISWIV; -.
DR PhylomeDB; Q554C9; -.
DR PRO; PR:Q554C9; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0052907; F:23S rRNA (adenine(1618)-N(6))-methyltransferase activity; IBA:GO_Central.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0070475; P:rRNA base methylation; IBA:GO_Central.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR017182; METTL16/PsiM.
DR InterPro; IPR010286; METTL16/RlmF.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR13393; PTHR13393; 1.
DR Pfam; PF05971; Methyltransf_10; 2.
DR PIRSF; PIRSF037350; Mtase_ZK1128_prd; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
PE 3: Inferred from homology;
KW Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW Transferase.
FT CHAIN 1..568
FT /note="U6 small nuclear RNA (adenine-(43)-N(6))-
FT methyltransferase"
FT /id="PRO_0000339419"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 363..383
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 403..431
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 503..538
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 504..538
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 117
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250|UniProtKB:Q86W50"
FT BINDING 151
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250|UniProtKB:Q86W50"
FT BINDING 175
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250|UniProtKB:Q86W50"
FT BINDING 250
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250|UniProtKB:Q86W50"
SQ SEQUENCE 568 AA; 66632 MW; DADD5D54630AD865 CRC64;
MSEIDTNDIK KEMDNKNYRD PTDNKELLKW TKKKRKRSND SMHINNFYRY NPPNFKLLAS
KYPTFDKYII NKTEKIYNID WKDSNATKEL TRVLLDHDFG LRIELPDNYL CPTLTLRINY
LYWISDQLKN LKIILNDNDN DNKIIKGIDI GTGTSCIFPL LGAKLFNNWS FIGIDIDDKV
LEYAQNNITI NSLNSKITLF KNEKNSDILL KLLNYKEGSN TFNSSNDDHQ DNHDDDDDDE
EYFADFCLCN PPFFKDLNEN NNNKNNNPKS NCTGSVNEMV TDGGEFEFVK RIIKESFQLK
CKIRFYTTMI GRKVNLNPLI NILIKQYYLP KNQIQTTELV QGNTSRWVLS WYFLNKSTNL
ETKENNNINN NNNNNNNNNI NNNNQFLTRM ERRKLYREGI TLNLDSDDNN NNNNNNNNNN
NNNNNNNNNN NNNNNKIVEI IKLILDNNDI IYKTDENNIK YECKYLLNNV IVGSSIKLDR
DIEFLFTIFI DLTTRLILFK PIDPKINNNN NNNNNNNNNN NNNNNNNNNN NNNNNKNNNN
SCDGEIINSN LFFILKFLEN IKNEIKLK
