ID   MET23_RAT               Reviewed;         225 AA.
AC   Q5RJL2;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Histone-arginine methyltransferase METTL23 {ECO:0000305};
DE            EC=2.1.1.319 {ECO:0000250|UniProtKB:A2AA28};
DE   AltName: Full=Methyltransferase-like protein 23 {ECO:0000305};
GN   Name=Mettl23 {ECO:0000312|RGD:1306284};
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Ovary;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Histone methyltransferase that dimethylates histone H3 at
CC       'Arg-17', forming asymmetric dimethylarginine (H3R17me2a), leading to
CC       activate transcription via chromatin remodeling. Maternal factor
CC       involved in epigenetic chromatin reprogramming of the paternal genome
CC       in the zygote: mediates H3R17me2a, promoting histone H3.3 incorporation
CC       in the male pronucleus, leading to TET3 recruitment and subsequent DNA
CC       demethylation. {ECO:0000250|UniProtKB:A2AA28}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-arginyl-[protein] + 2 S-adenosyl-L-methionine = 2 H(+) +
CC         N(omega),N(omega)-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-
CC         homocysteine; Xref=Rhea:RHEA:48096, Rhea:RHEA-COMP:10532, Rhea:RHEA-
CC         COMP:11991, ChEBI:CHEBI:15378, ChEBI:CHEBI:29965, ChEBI:CHEBI:57856,
CC         ChEBI:CHEBI:59789, ChEBI:CHEBI:61897; EC=2.1.1.319;
CC         Evidence={ECO:0000250|UniProtKB:A2AA28};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:48097;
CC         Evidence={ECO:0000250|UniProtKB:A2AA28};
CC   -!- SUBUNIT: Interacts with HSPA5, HSP90B1, TUBULIN, UGGT1 and UGGT2 (By
CC       similarity). Interacts with TET3. Interacts with STPG4 (By similarity).
CC       {ECO:0000250|UniProtKB:A2AA28, ECO:0000250|UniProtKB:Q86XA0}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:A2AA28}. Cytoplasm
CC       {ECO:0000250|UniProtKB:A2AA28}. Note=Localizes in male and female
CC       zygote pronucleus and cytoplasm. {ECO:0000250|UniProtKB:A2AA28}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. METTL23
CC       family. {ECO:0000305}.
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DR   EMBL; BC086594; AAH86594.1; -; mRNA.
DR   RefSeq; NP_001008284.1; NM_001008283.1.
DR   AlphaFoldDB; Q5RJL2; -.
DR   SMR; Q5RJL2; -.
DR   STRING; 10116.ENSRNOP00000000266; -.
DR   PhosphoSitePlus; Q5RJL2; -.
DR   PaxDb; Q5RJL2; -.
DR   Ensembl; ENSRNOT00000000266; ENSRNOP00000000266; ENSRNOG00000000249.
DR   GeneID; 287918; -.
DR   KEGG; rno:287918; -.
DR   CTD; 124512; -.
DR   RGD; 1306284; Mettl23.
DR   eggNOG; KOG2793; Eukaryota.
DR   GeneTree; ENSGT00510000048008; -.
DR   HOGENOM; CLU_082022_1_0_1; -.
DR   InParanoid; Q5RJL2; -.
DR   OMA; LMQDHTI; -.
DR   OrthoDB; 1588190at2759; -.
DR   PhylomeDB; Q5RJL2; -.
DR   PRO; PR:Q5RJL2; -.
DR   Proteomes; UP000002494; Chromosome 10.
DR   Bgee; ENSRNOG00000000249; Expressed in pancreas and 19 other tissues.
DR   Genevisible; Q5RJL2; RN.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0001939; C:female pronucleus; ISS:UniProtKB.
DR   GO; GO:0001940; C:male pronucleus; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISO:RGD.
DR   GO; GO:0032991; C:protein-containing complex; ISO:RGD.
DR   GO; GO:0140297; F:DNA-binding transcription factor binding; ISO:RGD.
DR   GO; GO:0031072; F:heat shock protein binding; ISO:RGD.
DR   GO; GO:0035642; F:histone methyltransferase activity (H3-R17 specific); ISS:UniProtKB.
DR   GO; GO:0035242; F:protein-arginine omega-N asymmetric methyltransferase activity; IEA:RHEA.
DR   GO; GO:0006325; P:chromatin organization; IEA:UniProtKB-KW.
DR   GO; GO:0044725; P:chromatin reprogramming in the zygote; ISS:UniProtKB.
DR   GO; GO:0050890; P:cognition; ISO:RGD.
DR   GO; GO:0044727; P:DNA demethylation of male pronucleus; ISS:UniProtKB.
DR   GO; GO:0034971; P:histone H3-R17 methylation; ISS:UniProtKB.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:RGD.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR019410; Methyltransf_16.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR14614; PTHR14614; 1.
DR   Pfam; PF10294; Methyltransf_16; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   2: Evidence at transcript level;
KW   Chromatin regulator; Cytoplasm; Developmental protein; Methyltransferase;
KW   Nucleus; Reference proteome; S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..225
FT                   /note="Histone-arginine methyltransferase METTL23"
FT                   /id="PRO_0000321522"
SQ   SEQUENCE   225 AA;  25277 MW;  DBAD08BF12FEF764 CRC64;
     MAGARLFRFR DEPGPGADPT VLEVRVPQVL HVQYGMYVWP CAVVLAQYLW FHRRVLPGKA
     VLEIGAGVSL PGILAAKCGA KVTLSDSPEL PHCLDICWQS CQMNNLPQVQ IVGLTWGHIS
     KDTLSLPPQD IILGSDVFFE PEDFESILAT VYFLMQKNPK VQFWSTYQVR SADWSLEGLL
     YKWDMRCVHI PLESFDADKE DIAESTLPGR HTIEMLIISF AKDSS