ID   MET2_SACPS              Reviewed;         486 AA.
AC   Q06736;
DT   11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Homoserine O-acetyltransferase;
DE            EC=2.3.1.31;
DE   AltName: Full=Homoserine O-trans-acetylase;
GN   Name=MET2;
OS   Saccharomyces pastorianus (Lager yeast) (Saccharomyces cerevisiae x
OS   Saccharomyces eubayanus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=27292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=M204;
RX   PubMed=8125336; DOI=10.1016/0378-1119(94)90727-7;
RA   Hansen J., Kielland-Brandt M.C.;
RT   "Saccharomyces carlsbergensis contains two functional MET2 alleles similar
RT   to homologues from S. cerevisiae and S. monacensis.";
RL   Gene 140:33-40(1994).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine;
CC         Xref=Rhea:RHEA:13701, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288,
CC         ChEBI:CHEBI:57476, ChEBI:CHEBI:57716; EC=2.3.1.31;
CC   -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de novo
CC       pathway; O-acetyl-L-homoserine from L-homoserine: step 1/1.
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. MetX family.
CC       {ECO:0000305}.
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DR   EMBL; L16688; AAB04108.1; -; Unassigned_DNA.
DR   AlphaFoldDB; Q06736; -.
DR   SMR; Q06736; -.
DR   ESTHER; yeast-met2; Homoserine_transacetylase.
DR   UniPathway; UPA00051; UER00074.
DR   GO; GO:0004414; F:homoserine O-acetyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   HAMAP; MF_00296; MetX_acyltransf; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   InterPro; IPR008220; HAT_MetX-like.
DR   PANTHER; PTHR32268; PTHR32268; 1.
DR   Pfam; PF00561; Abhydrolase_1; 1.
DR   PIRSF; PIRSF000443; Homoser_Ac_trans; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
DR   TIGRFAMs; TIGR01392; homoserO_Ac_trn; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Amino-acid biosynthesis; Methionine biosynthesis;
KW   Transferase.
FT   CHAIN           1..486
FT                   /note="Homoserine O-acetyltransferase"
FT                   /id="PRO_0000155757"
FT   DOMAIN          66..436
FT                   /note="AB hydrolase-1"
FT                   /evidence="ECO:0000255"
FT   REGION          248..274
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        162
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        430
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   486 AA;  53835 MW;  29C5CD6B50EDE27B CRC64;
     MSHTAKSKTL QELDIEDIKE TNPLLNLVQG QRIVQVPELV LESGVVLNNF PIAYKTWGTL
     NEACDNVLVI CHALTGSADV ADWWGPLLGN DLAFDPSRFF IICLNSMGSP YGSFSPLTIN
     EQTGTRYGPE FPLCTVRDDV RAHRIVLDSL GVKSIACVIG GSMGGMLSLE WTAMYGNEYV
     KNMVALATSA RHSAWCISWS EAQRQSIYSD PNYLDGYYPV EEQPVAGLSA ARMSALLTYR
     TRNSFENKFS RRSPSIAQQQ KAQKAEVRKP STVSEHSLQI HNDGYKCKAN GAIAGISGQK
     RQSVVSTTSS SDSLTSQSSM TSVSSVTGEV KDIKPAQTYF SAQSYLRYQG TKFINRFDAN
     CYIAITRKLD THDLARDRVE DITKVLSAIE QPSLIIGIQS DGLFTYSEQE FLAEYIPNSQ
     LEKIESPEGH DAFLLEFKLI NKLIVNFLKT NCKNIMDAKP RTWGGDVGND ETKTSVFGEA
     EEVTNW