ARL13_CHLRE
ID ARL13_CHLRE Reviewed; 527 AA.
AC A8INQ0;
DT 19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 1.
DT 03-AUG-2022, entry version 64.
DE RecName: Full=ADP-ribosylation factor-like protein 13B;
DE Short=CrArl13B;
GN Name=ARL13; ORFNames=CHLREDRAFT_195529;
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503;
RX PubMed=17932292; DOI=10.1126/science.1143609;
RA Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L.,
RA Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H.,
RA Kapitonov V.V., Ren Q., Ferris P., Lindquist E., Shapiro H., Lucas S.M.,
RA Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L.,
RA Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H.,
RA Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M.,
RA Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A.,
RA Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L.,
RA Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C.,
RA Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.J.,
RA Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L.,
RA Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L.,
RA Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R.,
RA Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P.,
RA Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y.,
RA Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L.,
RA Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.;
RT "The Chlamydomonas genome reveals the evolution of key animal and plant
RT functions.";
RL Science 318:245-250(2007).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 18-242 IN COMPLEX WITH GTP, AND
RP SUBUNIT.
RX PubMed=24168557; DOI=10.1042/bj20131097;
RA Miertzschke M., Koerner C., Spoerner M., Wittinghofer A.;
RT "Structural insights into the small G-protein Arl13B and implications for
RT Joubert syndrome.";
RL Biochem. J. 457:301-311(2014).
CC -!- FUNCTION: Cilium-specific protein required to control the microtubule-
CC based, ciliary axoneme structure. May act by maintaining the
CC association between IFT subcomplexes A and B (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:24168557}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium membrane {ECO:0000250}.
CC -!- DOMAIN: Contains an incomplete active site due to the presence of a Gly
CC residue in position 72 instead of a Gln, probably explaining the
CC inability to hydrolyze GTP. {ECO:0000269|PubMed:24168557}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Arf family.
CC {ECO:0000305}.
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DR EMBL; DS496119; EDP05163.1; -; Genomic_DNA.
DR RefSeq; XP_001691430.1; XM_001691378.1.
DR PDB; 4M9Q; X-ray; 2.50 A; A/B/C=18-242.
DR PDB; 5DI3; X-ray; 2.50 A; B=18-278.
DR PDBsum; 4M9Q; -.
DR PDBsum; 5DI3; -.
DR AlphaFoldDB; A8INQ0; -.
DR SMR; A8INQ0; -.
DR STRING; 3055.EDP05163; -.
DR PaxDb; A8INQ0; -.
DR PRIDE; A8INQ0; -.
DR EnsemblPlants; PNW82955; PNW82955; CHLRE_06g301050v5.
DR GeneID; 5716967; -.
DR Gramene; PNW82955; PNW82955; CHLRE_06g301050v5.
DR KEGG; cre:CHLRE_06g301050v5; -.
DR eggNOG; KOG0074; Eukaryota.
DR HOGENOM; CLU_517166_0_0_1; -.
DR InParanoid; A8INQ0; -.
DR OrthoDB; 732329at2759; -.
DR GO; GO:0060170; C:ciliary membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR006689; Small_GTPase_ARF/SAR.
DR Pfam; PF00025; Arf; 1.
DR PRINTS; PR00328; SAR1GTPBP.
DR SMART; SM00178; SAR; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51417; ARF; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Cell projection; Cilium; GTP-binding;
KW Membrane; Nucleotide-binding.
FT CHAIN 1..527
FT /note="ADP-ribosylation factor-like protein 13B"
FT /id="PRO_0000425900"
FT REGION 200..252
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 300..503
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 200..247
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 366..381
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 383..399
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 489..503
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 26..33
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000269|PubMed:24168557"
FT BINDING 69..73
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 128..131
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000269|PubMed:24168557"
FT STRAND 18..25
FT /evidence="ECO:0007829|PDB:4M9Q"
FT HELIX 32..39
FT /evidence="ECO:0007829|PDB:4M9Q"
FT STRAND 51..60
FT /evidence="ECO:0007829|PDB:4M9Q"
FT STRAND 63..70
FT /evidence="ECO:0007829|PDB:4M9Q"
FT TURN 74..76
FT /evidence="ECO:0007829|PDB:4M9Q"
FT HELIX 77..86
FT /evidence="ECO:0007829|PDB:4M9Q"
FT STRAND 88..95
FT /evidence="ECO:0007829|PDB:4M9Q"
FT HELIX 99..101
FT /evidence="ECO:0007829|PDB:4M9Q"
FT HELIX 102..114
FT /evidence="ECO:0007829|PDB:4M9Q"
FT HELIX 116..118
FT /evidence="ECO:0007829|PDB:4M9Q"
FT STRAND 123..128
FT /evidence="ECO:0007829|PDB:4M9Q"
FT HELIX 138..144
FT /evidence="ECO:0007829|PDB:4M9Q"
FT HELIX 147..149
FT /evidence="ECO:0007829|PDB:4M9Q"
FT STRAND 154..158
FT /evidence="ECO:0007829|PDB:4M9Q"
FT HELIX 172..205
FT /evidence="ECO:0007829|PDB:4M9Q"
FT HELIX 206..210
FT /evidence="ECO:0007829|PDB:4M9Q"
SQ SEQUENCE 527 AA; 54793 MW; F73AB844C85833DD CRC64;
MFGLLVNFYR FCRKKTERKI TIALLGLDNA GKTTLLNSIQ GEVDRDTTPT FGFNSTTLNE
GKYKIEVFDL GGGKNIRGVW KKYLAEVHAI VYVVDAADPG RFEESKMTMA EVLENQFMRD
KPICIFANKQ DLPTAAPAAE VVKGLGLATC RNSHNVFPCT AKMPAGQDVD HRLRDGLKWL
VGTVDREFGR LDPRVQTEAE EVRQEEARKK KEREERLRKQ REERLRQQKE EEERAREVEK
ENELHDGKAP SLLAAGGGVV GAAAAGVNGV MVDEQQELRP PGQHQEAPEA LGLHNGLALG
LPHTIESPGK FPPPPRRPLE AHPASDLRLV APDQGVSSAS GGPGLGAMPS GSHGGGGVPP
QPASLPHVRA ALPPLPPSAP QPSDAGVGSS GSASRHPGAH SSSAAAPPNV ASGAAAEDGP
EPDAAGTAAG EAGSGSVFAP RPASAGGGGP GSRGSGSGMT PDARELGSGG VESGEGTPAR
LRAGAQQASD GGHGNSKGSF SLVHTSNKVV PVAPDLRAGI PGAPNDA
