ID   META_SODGM              Reviewed;         309 AA.
AC   Q2NQZ8;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Probable acyltransferase {ECO:0000255|HAMAP-Rule:MF_00295};
DE            EC=2.3.1.- {ECO:0000255|HAMAP-Rule:MF_00295};
GN   Name=metA; OrderedLocusNames=SG2152;
OS   Sodalis glossinidius (strain morsitans).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Bruguierivoracaceae; Sodalis.
OX   NCBI_TaxID=343509;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=morsitans;
RX   PubMed=16365377; DOI=10.1101/gr.4106106;
RA   Toh H., Weiss B.L., Perkin S.A.H., Yamashita A., Oshima K., Hattori M.,
RA   Aksoy S.;
RT   "Massive genome erosion and functional adaptations provide insights into
RT   the symbiotic lifestyle of Sodalis glossinidius in the tsetse host.";
RL   Genome Res. 16:149-156(2006).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00295}.
CC   -!- SIMILARITY: Belongs to the MetA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00295}.
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DR   EMBL; AP008232; BAE75427.1; -; Genomic_DNA.
DR   RefSeq; WP_011411964.1; NZ_LN854557.1.
DR   AlphaFoldDB; Q2NQZ8; -.
DR   SMR; Q2NQZ8; -.
DR   STRING; 343509.SG2152; -.
DR   EnsemblBacteria; BAE75427; BAE75427; SG2152.
DR   KEGG; sgl:SG2152; -.
DR   eggNOG; COG1897; Bacteria.
DR   HOGENOM; CLU_057851_0_1_6; -.
DR   OMA; WLWFCYQ; -.
DR   OrthoDB; 1601420at2; -.
DR   BioCyc; SGLO343509:SGP1_RS19865-MON; -.
DR   Proteomes; UP000001932; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008899; F:homoserine O-succinyltransferase activity; IEA:InterPro.
DR   GO; GO:0019281; P:L-methionine biosynthetic process from homoserine via O-succinyl-L-homoserine and cystathionine; IEA:InterPro.
DR   CDD; cd03131; GATase1_HTS; 1.
DR   Gene3D; 3.40.50.880; -; 1.
DR   HAMAP; MF_00295; MetA_acyltransf; 1.
DR   InterPro; IPR029062; Class_I_gatase-like.
DR   InterPro; IPR005697; HST_MetA.
DR   InterPro; IPR033752; MetA_family.
DR   PANTHER; PTHR20919; PTHR20919; 1.
DR   Pfam; PF04204; HTS; 1.
DR   PIRSF; PIRSF000450; H_ser_succinyltr; 1.
DR   SUPFAM; SSF52317; SSF52317; 1.
DR   TIGRFAMs; TIGR01001; metA; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Amino-acid biosynthesis; Cytoplasm; Transferase.
FT   CHAIN           1..309
FT                   /note="Probable acyltransferase"
FT                   /id="PRO_1000021849"
FT   ACT_SITE        142
FT                   /note="Acyl-thioester intermediate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00295"
FT   ACT_SITE        235
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00295"
FT   ACT_SITE        237
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00295"
FT   BINDING         163
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00295"
FT   BINDING         192
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00295"
FT   BINDING         249
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00295"
FT   SITE            192
FT                   /note="Important for substrate specificity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00295"
SQ   SEQUENCE   309 AA;  35704 MW;  1414F2BFF5585117 CRC64;
     MPIRVFDELP AVNFLRNENV FVMTSSRAST QEIRSLKVLI LNLMPKKIET ENQFLRLLSN
     SPLQVDIQLL RIDSRESKNT PAEHLNNFYC NFEDIQHDNF DGLVVTGAPL SLVDFHDVVF
     WPQIDRVLHW ARDHVTSTLF VCWAVQAALN ILYGIPKMTR QQKLAGVYQH HLLKPHALLT
     RGFDETFLAP HSRNADFPTA MIRQHTDLEI LAESDEAGAY LFASHDKRLA FVTGHPEYDA
     LTLASEYHRD RENGLNPSLP VNYFLQDNPD LTPKASWRSH GHLLVANWLN YYVYQITPYD
     LRRMNPTLE