ID   METC_PAPLA              Reviewed;          17 AA.
AC   P85217;
DT   21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   25-MAY-2022, entry version 29.
DE   RecName: Full=Cystathionine beta lyase;
DE            EC=4.4.1.13;
DE   AltName: Full=Cysteine-S-conjugate beta-lyase;
DE   Flags: Fragment;
OS   Papiliotrema laurentii (Cryptococcus laurentii).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Rhynchogastremataceae; Papiliotrema.
OX   NCBI_TaxID=5418;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE.
RC   STRAIN=RY1 {ECO:0000269|Ref.1};
RA   Dutta D., Gachhui R.;
RT   "Functional nitrogenase in Cryptococcus laurentii strain RY1.";
RL   Submitted (JUL-2007) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + L,L-cystathionine = L-homocysteine + NH4(+) + pyruvate;
CC         Xref=Rhea:RHEA:13965, ChEBI:CHEBI:15361, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:28938, ChEBI:CHEBI:58161, ChEBI:CHEBI:58199;
CC         Evidence={ECO:0000250|UniProtKB:A0RIN4};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an S-substituted L-cysteine + H2O = a thiol + NH4(+) +
CC         pyruvate; Xref=Rhea:RHEA:18121, ChEBI:CHEBI:15361, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:28938, ChEBI:CHEBI:29256, ChEBI:CHEBI:58717; EC=4.4.1.13;
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000250|UniProtKB:A0RIN4};
CC   -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de novo
CC       pathway; L-homocysteine from L-cystathionine: step 1/1.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:A0RIN4}.
CC   -!- SIMILARITY: Belongs to the trans-sulfuration enzymes family.
CC       {ECO:0000255}.
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DR   AlphaFoldDB; P85217; -.
DR   UniPathway; UPA00051; UER00078.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004121; F:cystathionine beta-lyase activity; IEA:RHEA.
DR   GO; GO:0047804; F:cysteine-S-conjugate beta-lyase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amino-acid biosynthesis; Cytoplasm; Direct protein sequencing; Lyase;
KW   Methionine biosynthesis.
FT   CHAIN           <1..>17
FT                   /note="Cystathionine beta lyase"
FT                   /id="PRO_0000298774"
FT   NON_TER         1
FT                   /evidence="ECO:0000303|Ref.1"
FT   NON_TER         17
FT                   /evidence="ECO:0000303|Ref.1"
SQ   SEQUENCE   17 AA;  1864 MW;  16A24179667D5E7A CRC64;
     HDVYGGDYRI TGDDGHS