ARL5A_HUMAN
ID ARL5A_HUMAN Reviewed; 179 AA.
AC Q9Y689; Q580I5;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 189.
DE RecName: Full=ADP-ribosylation factor-like protein 5A;
GN Name=ARL5A; Synonyms=ARFLP5, ARL5;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=12450215; DOI=10.3727/000000002783992406;
RA He H., Dai F.Y., Yu L., She X., Zhao Y., Jiang J., Chen X., Zhao S.Y.;
RT "Identification and characterization of nine novel human small GTPases
RT showing variable expressions in liver cancer tissues.";
RL Gene Expr. 10:231-242(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Pituitary;
RX PubMed=10931946; DOI=10.1073/pnas.160270997;
RA Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X.,
RA Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W.,
RA Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M., Zhou J., Xu S.-H., Gu J.,
RA Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z.,
RA Chen M.-D., Chen J.-L.;
RT "Gene expression profiling in the human hypothalamus-pituitary-adrenal axis
RT and full-length cDNA cloning.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Thymus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RA Puhl H.L. III, Ikeda S.R., Aronstam R.S.;
RT "cDNA clones of human proteins involved in signal transduction sequenced by
RT the Guthrie cDNA resource center (www.cdna.org).";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA Phelan M., Farmer A.;
RT "Cloning of human full-length CDSs in BD Creator(TM) system donor vector.";
RL Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RA Li W.B., Gruber C., Jessee J., Polayes D.;
RT "Full-length cDNA libraries and normalization.";
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [9]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [10]
RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH GTP ANALOG.
RX PubMed=15896705; DOI=10.1016/j.bbrc.2005.04.168;
RA Wang Z.-X., Shi L., Liu J.-F., An X.-M., Chang W.-R., Liang D.-C.;
RT "2.0 A crystal structure of human ARL5-GDP3'P, a novel member of the small
RT GTP-binding proteins.";
RL Biochem. Biophys. Res. Commun. 332:640-645(2005).
RN [11]
RP X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 15-176.
RG Structural genomics consortium (SGC);
RT "Structure of human ADP-ribosylation factor-like 5 (ARL5).";
RL Submitted (JUN-2006) to the PDB data bank.
CC -!- FUNCTION: Lacks ADP-ribosylation enhancing activity. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9Y689-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9Y689-2; Sequence=VSP_041017;
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Arf family.
CC {ECO:0000305}.
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DR EMBL; AF087889; AAP97188.1; -; mRNA.
DR EMBL; AF100740; AAD40383.1; -; mRNA.
DR EMBL; AK292714; BAF85403.1; -; mRNA.
DR EMBL; AF493891; AAM12605.1; -; mRNA.
DR EMBL; BT009829; AAP88831.1; -; mRNA.
DR EMBL; CR613929; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AC097448; AAX82013.1; -; Genomic_DNA.
DR EMBL; CH471058; EAX11495.1; -; Genomic_DNA.
DR EMBL; CH471058; EAX11496.1; -; Genomic_DNA.
DR EMBL; BC001254; AAH01254.1; -; mRNA.
DR CCDS; CCDS2195.1; -. [Q9Y689-1]
DR CCDS; CCDS46425.1; -. [Q9Y689-2]
DR RefSeq; NP_001032251.1; NM_001037174.1. [Q9Y689-2]
DR RefSeq; NP_036229.1; NM_012097.3. [Q9Y689-1]
DR RefSeq; NP_817114.2; NM_177985.2. [Q9Y689-2]
DR PDB; 1Z6Y; X-ray; 2.40 A; A/B=1-179.
DR PDB; 1ZJ6; X-ray; 2.00 A; A=1-179.
DR PDB; 2H16; X-ray; 2.00 A; A/B/C/D=14-177.
DR PDB; 2H17; X-ray; 1.70 A; A=15-176.
DR PDBsum; 1Z6Y; -.
DR PDBsum; 1ZJ6; -.
DR PDBsum; 2H16; -.
DR PDBsum; 2H17; -.
DR AlphaFoldDB; Q9Y689; -.
DR SMR; Q9Y689; -.
DR BioGRID; 117616; 57.
DR STRING; 9606.ENSP00000295087; -.
DR DrugBank; DB04315; Guanosine-5'-Diphosphate.
DR iPTMnet; Q9Y689; -.
DR PhosphoSitePlus; Q9Y689; -.
DR BioMuta; ARL5A; -.
DR DMDM; 12229600; -.
DR EPD; Q9Y689; -.
DR jPOST; Q9Y689; -.
DR MassIVE; Q9Y689; -.
DR MaxQB; Q9Y689; -.
DR PaxDb; Q9Y689; -.
DR PeptideAtlas; Q9Y689; -.
DR PRIDE; Q9Y689; -.
DR ProteomicsDB; 86622; -. [Q9Y689-1]
DR ProteomicsDB; 86623; -. [Q9Y689-2]
DR Antibodypedia; 33675; 65 antibodies from 16 providers.
DR DNASU; 26225; -.
DR Ensembl; ENST00000295087.13; ENSP00000295087.8; ENSG00000162980.17. [Q9Y689-1]
DR Ensembl; ENST00000428992.2; ENSP00000415950.1; ENSG00000162980.17. [Q9Y689-2]
DR Ensembl; ENST00000446896.7; ENSP00000394626.3; ENSG00000162980.17. [Q9Y689-2]
DR GeneID; 26225; -.
DR KEGG; hsa:26225; -.
DR MANE-Select; ENST00000295087.13; ENSP00000295087.8; NM_012097.4; NP_036229.1.
DR UCSC; uc002txw.2; human. [Q9Y689-1]
DR CTD; 26225; -.
DR DisGeNET; 26225; -.
DR GeneCards; ARL5A; -.
DR HGNC; HGNC:696; ARL5A.
DR HPA; ENSG00000162980; Low tissue specificity.
DR MIM; 608960; gene.
DR neXtProt; NX_Q9Y689; -.
DR OpenTargets; ENSG00000162980; -.
DR PharmGKB; PA24989; -.
DR VEuPathDB; HostDB:ENSG00000162980; -.
DR eggNOG; KOG0070; Eukaryota.
DR GeneTree; ENSGT00940000154714; -.
DR HOGENOM; CLU_040729_14_2_1; -.
DR InParanoid; Q9Y689; -.
DR OMA; IWRLFSH; -.
DR OrthoDB; 1417432at2759; -.
DR PhylomeDB; Q9Y689; -.
DR TreeFam; TF105465; -.
DR PathwayCommons; Q9Y689; -.
DR SignaLink; Q9Y689; -.
DR BioGRID-ORCS; 26225; 20 hits in 1010 CRISPR screens.
DR ChiTaRS; ARL5A; human.
DR EvolutionaryTrace; Q9Y689; -.
DR GenomeRNAi; 26225; -.
DR Pharos; Q9Y689; Tbio.
DR PRO; PR:Q9Y689; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q9Y689; protein.
DR Bgee; ENSG00000162980; Expressed in calcaneal tendon and 195 other tissues.
DR ExpressionAtlas; Q9Y689; baseline and differential.
DR Genevisible; Q9Y689; HS.
DR GO; GO:0005802; C:trans-Golgi network; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IBA:GO_Central.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:1903292; P:protein localization to Golgi membrane; IGI:FlyBase.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR024156; Small_GTPase_ARF.
DR InterPro; IPR006689; Small_GTPase_ARF/SAR.
DR PANTHER; PTHR11711; PTHR11711; 1.
DR Pfam; PF00025; Arf; 1.
DR PRINTS; PR00328; SAR1GTPBP.
DR SMART; SM00178; SAR; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51417; ARF; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; GTP-binding; Lipoprotein; Myristate;
KW Nucleotide-binding; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000255"
FT CHAIN 2..179
FT /note="ADP-ribosylation factor-like protein 5A"
FT /id="PRO_0000207467"
FT BINDING 23..30
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT BINDING 66..70
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 125..128
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT BINDING 159
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..37
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.6"
FT /id="VSP_041017"
FT HELIX 3..12
FT /evidence="ECO:0007829|PDB:1ZJ6"
FT STRAND 17..24
FT /evidence="ECO:0007829|PDB:2H17"
FT HELIX 29..37
FT /evidence="ECO:0007829|PDB:2H17"
FT STRAND 42..45
FT /evidence="ECO:0007829|PDB:2H17"
FT STRAND 48..51
FT /evidence="ECO:0007829|PDB:2H17"
FT STRAND 53..57
FT /evidence="ECO:0007829|PDB:2H17"
FT STRAND 60..70
FT /evidence="ECO:0007829|PDB:2H17"
FT HELIX 71..73
FT /evidence="ECO:0007829|PDB:2H17"
FT HELIX 76..81
FT /evidence="ECO:0007829|PDB:2H17"
FT STRAND 86..92
FT /evidence="ECO:0007829|PDB:2H17"
FT TURN 96..98
FT /evidence="ECO:0007829|PDB:2H17"
FT HELIX 99..110
FT /evidence="ECO:0007829|PDB:2H17"
FT HELIX 113..115
FT /evidence="ECO:0007829|PDB:2H17"
FT STRAND 119..125
FT /evidence="ECO:0007829|PDB:2H17"
FT HELIX 135..141
FT /evidence="ECO:0007829|PDB:2H17"
FT HELIX 144..146
FT /evidence="ECO:0007829|PDB:2H17"
FT STRAND 152..156
FT /evidence="ECO:0007829|PDB:2H17"
FT TURN 159..162
FT /evidence="ECO:0007829|PDB:2H17"
FT HELIX 165..173
FT /evidence="ECO:0007829|PDB:2H17"
SQ SEQUENCE 179 AA; 20728 MW; 431CC65F8D8CB9D5 CRC64;
MGILFTRIWR LFNHQEHKVI IVGLDNAGKT TILYQFSMNE VVHTSPTIGS NVEEIVINNT
RFLMWDIGGQ ESLRSSWNTY YTNTEFVIVV VDSTDRERIS VTREELYKML AHEDLRKAGL
LIFANKQDVK ECMTVAEISQ FLKLTSIKDH QWHIQACCAL TGEGLCQGLE WMMSRLKIR
