ARL5B_HUMAN
ID ARL5B_HUMAN Reviewed; 179 AA.
AC Q96KC2;
DT 19-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=ADP-ribosylation factor-like protein 5B;
DE AltName: Full=ADP-ribosylation factor-like protein 8;
GN Name=ARL5B; Synonyms=ARL8;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Fetal cartilage;
RX PubMed=12853149; DOI=10.1016/s0378-1119(03)00584-5;
RA Sebald E., Krueger R., King L.M., Cohn D.H., Krakow D.;
RT "Isolation of a new member of the ADP-ribosylation like factor gene family,
RT ARL8, from a cartilage cDNA library.";
RL Gene 311:147-151(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Prostatic adenocarcinoma;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH GDP.
RG Structural genomics consortium (SGC);
RT "Structure of human ADP-ribosylation factor-like 8.";
RL Submitted (JUN-2005) to the PDB data bank.
CC -!- FUNCTION: Binds and exchanges GTP and GDP.
CC -!- INTERACTION:
CC Q96KC2; Q9BVV2: FNDC11; NbExp=5; IntAct=EBI-12885454, EBI-744935;
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Arf family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF494061; AAM74076.1; -; mRNA.
DR EMBL; AK027275; BAB55011.1; -; mRNA.
DR EMBL; BC024163; AAH24163.1; -; mRNA.
DR CCDS; CCDS7131.1; -.
DR RefSeq; NP_848930.1; NM_178815.3.
DR PDB; 1YZG; X-ray; 2.00 A; A=1-179.
DR PDBsum; 1YZG; -.
DR AlphaFoldDB; Q96KC2; -.
DR SMR; Q96KC2; -.
DR BioGRID; 128683; 61.
DR IntAct; Q96KC2; 18.
DR STRING; 9606.ENSP00000366487; -.
DR DrugBank; DB04315; Guanosine-5'-Diphosphate.
DR iPTMnet; Q96KC2; -.
DR PhosphoSitePlus; Q96KC2; -.
DR BioMuta; ARL5B; -.
DR DMDM; 34922029; -.
DR EPD; Q96KC2; -.
DR jPOST; Q96KC2; -.
DR MassIVE; Q96KC2; -.
DR MaxQB; Q96KC2; -.
DR PaxDb; Q96KC2; -.
DR PeptideAtlas; Q96KC2; -.
DR PRIDE; Q96KC2; -.
DR ProteomicsDB; 77058; -.
DR Antibodypedia; 25424; 108 antibodies from 22 providers.
DR DNASU; 221079; -.
DR Ensembl; ENST00000377275.4; ENSP00000366487.3; ENSG00000165997.5.
DR GeneID; 221079; -.
DR KEGG; hsa:221079; -.
DR MANE-Select; ENST00000377275.4; ENSP00000366487.3; NM_178815.5; NP_848930.1.
DR UCSC; uc001iqd.2; human.
DR CTD; 221079; -.
DR DisGeNET; 221079; -.
DR GeneCards; ARL5B; -.
DR HGNC; HGNC:23052; ARL5B.
DR HPA; ENSG00000165997; Low tissue specificity.
DR MIM; 608909; gene.
DR neXtProt; NX_Q96KC2; -.
DR OpenTargets; ENSG00000165997; -.
DR PharmGKB; PA134979161; -.
DR VEuPathDB; HostDB:ENSG00000165997; -.
DR eggNOG; KOG0070; Eukaryota.
DR GeneTree; ENSGT00940000157567; -.
DR HOGENOM; CLU_040729_9_1_1; -.
DR InParanoid; Q96KC2; -.
DR OMA; NIRLLMW; -.
DR OrthoDB; 1417432at2759; -.
DR PhylomeDB; Q96KC2; -.
DR TreeFam; TF105465; -.
DR PathwayCommons; Q96KC2; -.
DR SignaLink; Q96KC2; -.
DR BioGRID-ORCS; 221079; 15 hits in 1079 CRISPR screens.
DR ChiTaRS; ARL5B; human.
DR EvolutionaryTrace; Q96KC2; -.
DR GenomeRNAi; 221079; -.
DR Pharos; Q96KC2; Tbio.
DR PRO; PR:Q96KC2; -.
DR Proteomes; UP000005640; Chromosome 10.
DR RNAct; Q96KC2; protein.
DR Bgee; ENSG00000165997; Expressed in vena cava and 195 other tissues.
DR ExpressionAtlas; Q96KC2; baseline and differential.
DR Genevisible; Q96KC2; HS.
DR GO; GO:0005802; C:trans-Golgi network; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IBA:GO_Central.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:1903292; P:protein localization to Golgi membrane; IMP:FlyBase.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR024156; Small_GTPase_ARF.
DR InterPro; IPR006689; Small_GTPase_ARF/SAR.
DR PANTHER; PTHR11711; PTHR11711; 1.
DR Pfam; PF00025; Arf; 1.
DR PRINTS; PR00328; SAR1GTPBP.
DR SMART; SM00178; SAR; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51417; ARF; 1.
PE 1: Evidence at protein level;
KW 3D-structure; GTP-binding; Lipoprotein; Myristate; Nucleotide-binding;
KW Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000255"
FT CHAIN 2..179
FT /note="ADP-ribosylation factor-like protein 5B"
FT /id="PRO_0000207470"
FT BINDING 23..30
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT BINDING 31
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT BINDING 66..70
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 125..128
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT BINDING 159
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000255"
FT HELIX 7..14
FT /evidence="ECO:0007829|PDB:1YZG"
FT STRAND 18..23
FT /evidence="ECO:0007829|PDB:1YZG"
FT HELIX 29..37
FT /evidence="ECO:0007829|PDB:1YZG"
FT STRAND 53..57
FT /evidence="ECO:0007829|PDB:1YZG"
FT STRAND 60..66
FT /evidence="ECO:0007829|PDB:1YZG"
FT HELIX 71..77
FT /evidence="ECO:0007829|PDB:1YZG"
FT HELIX 78..81
FT /evidence="ECO:0007829|PDB:1YZG"
FT STRAND 86..92
FT /evidence="ECO:0007829|PDB:1YZG"
FT HELIX 96..98
FT /evidence="ECO:0007829|PDB:1YZG"
FT HELIX 99..110
FT /evidence="ECO:0007829|PDB:1YZG"
FT HELIX 113..115
FT /evidence="ECO:0007829|PDB:1YZG"
FT STRAND 119..125
FT /evidence="ECO:0007829|PDB:1YZG"
FT HELIX 135..141
FT /evidence="ECO:0007829|PDB:1YZG"
FT HELIX 144..146
FT /evidence="ECO:0007829|PDB:1YZG"
FT STRAND 152..156
FT /evidence="ECO:0007829|PDB:1YZG"
FT TURN 159..162
FT /evidence="ECO:0007829|PDB:1YZG"
FT HELIX 165..172
FT /evidence="ECO:0007829|PDB:1YZG"
SQ SEQUENCE 179 AA; 20375 MW; ECD74C4EA1A80E33 CRC64;
MGLIFAKLWS LFCNQEHKVI IVGLDNAGKT TILYQFLMNE VVHTSPTIGS NVEEIVVKNT
HFLMWDIGGQ ESLRSSWNTY YSNTEFIILV VDSIDRERLA ITKEELYRML AHEDLRKAAV
LIFANKQDMK GCMTAAEISK YLTLSSIKDH PWHIQSCCAL TGEGLCQGLE WMTSRIGVR
