ID   METJ_ECOL6              Reviewed;         105 AA.
AC   P0A8U7; P08338;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Met repressor;
DE   AltName: Full=Met regulon regulatory protein MetJ;
GN   Name=metJ; OrderedLocusNames=c4891;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
CC   -!- FUNCTION: This regulatory protein, when combined with SAM (S-
CC       adenosylmethionine) represses the expression of the methionine regulon
CC       and of enzymes involved in SAM synthesis. {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- DOMAIN: Does not bind DNA by a helix-turn-helix motif.
CC   -!- SIMILARITY: Belongs to the MetJ family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAN83319.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE014075; AAN83319.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_000852812.1; NC_004431.1.
DR   AlphaFoldDB; P0A8U7; -.
DR   SMR; P0A8U7; -.
DR   STRING; 199310.c4891; -.
DR   EnsemblBacteria; AAN83319; AAN83319; c4891.
DR   GeneID; 67417544; -.
DR   KEGG; ecc:c4891; -.
DR   eggNOG; COG3060; Bacteria.
DR   HOGENOM; CLU_142318_0_0_6; -.
DR   OMA; KWNGEYI; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00490; Met_repressor_MetJ; 1.
DR   Gene3D; 1.10.140.10; -; 1.
DR   HAMAP; MF_00744; MetJ; 1.
DR   InterPro; IPR002084; Met_repressor_MetJ.
DR   InterPro; IPR023453; Met_repressor_MetJ_dom_sf.
DR   InterPro; IPR010985; Ribbon_hlx_hlx.
DR   Pfam; PF01340; MetJ; 1.
DR   SUPFAM; SSF47598; SSF47598; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Cytoplasm; DNA-binding; Methionine biosynthesis;
KW   Repressor; Transcription; Transcription regulation.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..105
FT                   /note="Met repressor"
FT                   /id="PRO_0000198398"
SQ   SEQUENCE   105 AA;  12141 MW;  05B0F54918C697EF CRC64;
     MAEWSGEYIS PYAEHGKKSE QVKKITVSIP LKVLKILTDE RTRRQVNNLR HATNSELLCE
     AFLHAFTGQP LPDDADLRKE RSDEIPEAAK EIMREMGINP ETWEY