ARL8A_ARATH
ID ARL8A_ARATH Reviewed; 184 AA.
AC Q8VY57; Q9M2X1;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=ADP-ribosylation factor-like protein 8a {ECO:0000303|PubMed:22174675};
DE Short=AtARL8a {ECO:0000303|PubMed:22174675};
DE AltName: Full=ADP-ribosylation factor-like A1C {ECO:0000303|PubMed:12644670};
DE Short=AtARLA1C {ECO:0000303|PubMed:12644670};
GN Name=ARL8A {ECO:0000303|PubMed:22174675};
GN Synonyms=ARLA1C {ECO:0000303|PubMed:12644670};
GN OrderedLocusNames=At3g49870 {ECO:0000312|Araport:AT3G49870};
GN ORFNames=T16K5.220 {ECO:0000312|EMBL:CAB66925.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12644670; DOI=10.1104/pp.013052;
RA Vernoud V., Horton A.C., Yang Z., Nielsen E.;
RT "Analysis of the small GTPase gene superfamily of Arabidopsis.";
RL Plant Physiol. 131:1191-1208(2003).
RN [5]
RP GENE FAMILY.
RX PubMed=15723828; DOI=10.1093/jxb/eri099;
RA Gebbie L.K., Burn J.E., Hocart C.H., Williamson R.E.;
RT "Genes encoding ADP-ribosylation factors in Arabidopsis thaliana; genome
RT analysis and antisense suppression.";
RL J. Exp. Bot. 56:1079-1091(2005).
RN [6]
RP FUNCTION (MICROBIAL INFECTION), DISRUPTION PHENOTYPE, GENE FAMILY, AND
RP NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=22174675; DOI=10.1371/journal.ppat.1002409;
RA Nishikiori M., Mori M., Dohi K., Okamura H., Katoh E., Naito S., Meshi T.,
RA Ishikawa M.;
RT "A host small GTP-binding protein ARL8 plays crucial roles in tobamovirus
RT RNA replication.";
RL PLoS Pathog. 7:E1002409-E1002409(2011).
CC -!- FUNCTION: May play a role in lysosome motility. May play a role in
CC chromosome segregation. {ECO:0000250|UniProtKB:Q9NVJ2}.
CC -!- FUNCTION: (Microbial infection) Component of tomato mosaic virus (ToMV)
CC RNA replication complexes. Required for tobamovirus multiplication,
CC especially for efficient negative-strand RNA synthesis and viral RNA
CC capping. {ECO:0000269|PubMed:22174675}.
CC -!- SUBUNIT: Interacts with tubulin. {ECO:0000250|UniProtKB:Q9NVJ2}.
CC -!- SUBCELLULAR LOCATION: Late endosome membrane
CC {ECO:0000250|UniProtKB:Q9NVJ2}. Lysosome membrane
CC {ECO:0000250|UniProtKB:Q9NVJ2}. Cytoplasm, cytoskeleton, spindle
CC {ECO:0000250|UniProtKB:Q9NVJ2}. Note=Localizes with microtubules at the
CC spindle mid-zone during mitosis. {ECO:0000250|UniProtKB:Q9NVJ2}.
CC -!- DISRUPTION PHENOTYPE: In double and triple mutants arl8a-1 arl8b-1 and
CC arl8a-1 arl8b-1 arl8c-1, impaired multiplication of tomato mosaic virus
CC (ToMV) associated with reduced production of negative-strand RNA.
CC {ECO:0000269|PubMed:22174675}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Arf family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB66925.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL132965; CAB66925.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE78600.1; -; Genomic_DNA.
DR EMBL; AY072429; AAL62421.1; -; mRNA.
DR EMBL; AY114718; AAM48037.1; -; mRNA.
DR PIR; T46053; T46053.
DR RefSeq; NP_190556.2; NM_114847.5.
DR AlphaFoldDB; Q8VY57; -.
DR SMR; Q8VY57; -.
DR STRING; 3702.AT3G49870.1; -.
DR PaxDb; Q8VY57; -.
DR PRIDE; Q8VY57; -.
DR ProteomicsDB; 246994; -.
DR EnsemblPlants; AT3G49870.1; AT3G49870.1; AT3G49870.
DR GeneID; 824149; -.
DR Gramene; AT3G49870.1; AT3G49870.1; AT3G49870.
DR KEGG; ath:AT3G49870; -.
DR Araport; AT3G49870; -.
DR TAIR; locus:2097355; AT3G49870.
DR eggNOG; KOG0075; Eukaryota.
DR HOGENOM; CLU_040729_10_1_1; -.
DR InParanoid; Q8VY57; -.
DR OMA; GREVSCY; -.
DR OrthoDB; 1123043at2759; -.
DR PhylomeDB; Q8VY57; -.
DR PRO; PR:Q8VY57; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8VY57; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; HDA:TAIR.
DR GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; ISS:TAIR.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
DR GO; GO:0051607; P:defense response to virus; IGI:TAIR.
DR GO; GO:0015031; P:protein transport; IEA:InterPro.
DR CDD; cd04159; Arl10_like; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR044154; Arl8a/8b.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR006689; Small_GTPase_ARF/SAR.
DR Pfam; PF00025; Arf; 1.
DR PRINTS; PR00328; SAR1GTPBP.
DR SMART; SM00178; SAR; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51417; ARF; 1.
PE 2: Evidence at transcript level;
KW Cell cycle; Cell division; Chromosome partition; Cytoplasm; Cytoskeleton;
KW Endosome; GTP-binding; Lysosome; Membrane; Mitosis; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..184
FT /note="ADP-ribosylation factor-like protein 8a"
FT /id="PRO_0000438003"
FT INTRAMEM 1..18
FT /note="Note=Mediates targeting to membranes"
FT /evidence="ECO:0000250|UniProtKB:Q9NVJ2"
FT BINDING 29..34
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P62330"
FT BINDING 48..51
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P62330"
FT BINDING 70..74
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P62330"
FT BINDING 129..132
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P62330"
SQ SEQUENCE 184 AA; 20404 MW; F0492E876261BEF1 CRC64;
MGLLEAFLNW LRSLFFKQEM ELSLIGLQNA GKTSLVNVVA TGGYSEDMIP TVGFNMRKVT
KGNVTIKLWD LGGQPRFRSM WERYCRAVSA IVYVVDAADP DNLSVSKSEL HDLLSKTSLN
GIPLLVLGNK IDKPGALSKE ALTDEMGLTS LTDREVCCFM ISCKNSTNID QVIDWLVKHS
KSKN
