ARL8B_ARATH
ID ARL8B_ARATH Reviewed; 184 AA.
AC Q93Y31; Q9FJW7;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=ADP-ribosylation factor-like protein 8b {ECO:0000303|PubMed:22174675};
DE Short=AtARL8b {ECO:0000303|PubMed:22174675};
DE AltName: Full=ADP-ribosylation factor-like A1D {ECO:0000303|PubMed:12644670};
DE Short=AtARLA1D {ECO:0000303|PubMed:12644670};
GN Name=ARL8B {ECO:0000303|PubMed:22174675};
GN Synonyms=ARLA1D {ECO:0000303|PubMed:12644670};
GN OrderedLocusNames=At5g67560 {ECO:0000312|Araport:AT5G67560};
GN ORFNames=K9I9.13 {ECO:0000312|EMBL:BAB08464.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9734815; DOI=10.1093/dnares/5.3.203;
RA Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence
RT features of the regions of 1,367,185 bp covered by 19 physically assigned
RT P1 and TAC clones.";
RL DNA Res. 5:203-216(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12644670; DOI=10.1104/pp.013052;
RA Vernoud V., Horton A.C., Yang Z., Nielsen E.;
RT "Analysis of the small GTPase gene superfamily of Arabidopsis.";
RL Plant Physiol. 131:1191-1208(2003).
RN [6]
RP GENE FAMILY.
RX PubMed=15723828; DOI=10.1093/jxb/eri099;
RA Gebbie L.K., Burn J.E., Hocart C.H., Williamson R.E.;
RT "Genes encoding ADP-ribosylation factors in Arabidopsis thaliana; genome
RT analysis and antisense suppression.";
RL J. Exp. Bot. 56:1079-1091(2005).
RN [7]
RP FUNCTION (MICROBIAL INFECTION), DISRUPTION PHENOTYPE, GENE FAMILY, AND
RP NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=22174675; DOI=10.1371/journal.ppat.1002409;
RA Nishikiori M., Mori M., Dohi K., Okamura H., Katoh E., Naito S., Meshi T.,
RA Ishikawa M.;
RT "A host small GTP-binding protein ARL8 plays crucial roles in tobamovirus
RT RNA replication.";
RL PLoS Pathog. 7:E1002409-E1002409(2011).
CC -!- FUNCTION: May play a role in lysosome motility. May play a role in
CC chromosome segregation. {ECO:0000250|UniProtKB:Q9NVJ2}.
CC -!- FUNCTION: (Microbial infection) Component of tomato mosaic virus (ToMV)
CC RNA replication complexes. Required for tobamovirus multiplication,
CC especially for efficient negative-strand RNA synthesis and viral RNA
CC capping. {ECO:0000269|PubMed:22174675}.
CC -!- SUBUNIT: Interacts with tubulin. {ECO:0000250|UniProtKB:Q9NVJ2}.
CC -!- SUBCELLULAR LOCATION: Late endosome membrane
CC {ECO:0000250|UniProtKB:Q9NVJ2}. Lysosome membrane
CC {ECO:0000250|UniProtKB:Q9NVJ2}. Cytoplasm, cytoskeleton, spindle
CC {ECO:0000250|UniProtKB:Q9NVJ2}. Note=Localizes with microtubules at the
CC spindle mid-zone during mitosis. {ECO:0000250|UniProtKB:Q9NVJ2}.
CC -!- DISRUPTION PHENOTYPE: In double and triple mutants arl8a-1 arl8b-1 and
CC arl8a-1 arl8b-1 arl8c-1, impaired multiplication of tomato mosaic virus
CC (ToMV). {ECO:0000269|PubMed:22174675}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Arf family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB08464.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB013390; BAB08464.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED98360.1; -; Genomic_DNA.
DR EMBL; AY054524; AAK96715.1; -; mRNA.
DR EMBL; AY064616; AAL47331.1; -; mRNA.
DR EMBL; AY087487; AAM65030.1; -; mRNA.
DR RefSeq; NP_569051.1; NM_126156.5.
DR AlphaFoldDB; Q93Y31; -.
DR SMR; Q93Y31; -.
DR STRING; 3702.AT5G67560.1; -.
DR PaxDb; Q93Y31; -.
DR PRIDE; Q93Y31; -.
DR ProteomicsDB; 246599; -.
DR EnsemblPlants; AT5G67560.1; AT5G67560.1; AT5G67560.
DR GeneID; 836892; -.
DR Gramene; AT5G67560.1; AT5G67560.1; AT5G67560.
DR KEGG; ath:AT5G67560; -.
DR Araport; AT5G67560; -.
DR TAIR; locus:2158591; AT5G67560.
DR eggNOG; KOG0075; Eukaryota.
DR HOGENOM; CLU_040729_10_1_1; -.
DR InParanoid; Q93Y31; -.
DR OMA; QDMNSPE; -.
DR OrthoDB; 1123043at2759; -.
DR PhylomeDB; Q93Y31; -.
DR PRO; PR:Q93Y31; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q93Y31; baseline and differential.
DR GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; ISS:TAIR.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:InterPro.
DR CDD; cd04159; Arl10_like; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR044154; Arl8a/8b.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR006689; Small_GTPase_ARF/SAR.
DR Pfam; PF00025; Arf; 1.
DR PRINTS; PR00328; SAR1GTPBP.
DR SMART; SM00178; SAR; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51417; ARF; 1.
PE 2: Evidence at transcript level;
KW Cell cycle; Cell division; Chromosome partition; Cytoplasm; Cytoskeleton;
KW Endosome; GTP-binding; Lysosome; Membrane; Mitosis; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..184
FT /note="ADP-ribosylation factor-like protein 8b"
FT /id="PRO_0000438004"
FT INTRAMEM 1..18
FT /note="Note=Mediates targeting to membranes"
FT /evidence="ECO:0000250|UniProtKB:Q9NVJ2"
FT BINDING 29..34
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P62330"
FT BINDING 48..51
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P62330"
FT BINDING 70..74
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P62330"
FT BINDING 129..132
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P62330"
SQ SEQUENCE 184 AA; 20404 MW; 0E061A81FA084CCE CRC64;
MGLWDALLNW LRSLFFKQEM ELSLIGLQNA GKTSLVNVVA TGGYSEDMIP TVGFNMRKVT
KGSVTIKLWD LGGQPRFRSM WERYCRSVSA IVYVVDAADP DNLSVSKSEL HDLLSKTSLN
GIPLLVLGNK IDKPGALSKE ALTDEMGLKS LTDREVCCFM ISCKNSTNID QVIDWLVKHS
KSSN
