ARL8C_ARATH
ID ARL8C_ARATH Reviewed; 184 AA.
AC Q8W4C8; Q9FHQ2;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=ADP-ribosylation factor-like protein 8c {ECO:0000303|PubMed:22174675};
DE Short=AtARL8c {ECO:0000303|PubMed:22174675};
DE AltName: Full=ADP-ribosylation factor-like A1A {ECO:0000303|PubMed:12644670};
DE Short=AtARLA1A {ECO:0000303|PubMed:12644670};
GN Name=ARL8C {ECO:0000303|PubMed:22174675};
GN Synonyms=ARLA1A {ECO:0000303|PubMed:12644670};
GN OrderedLocusNames=At5g37680 {ECO:0000312|Araport:AT5G37680};
GN ORFNames=K12B20.15 {ECO:0000312|EMBL:BAB08314.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10470850; DOI=10.1093/dnares/6.3.183;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence
RT features of the regions of 1,011,550 bp covered by seventeen P1 and TAC
RT clones.";
RL DNA Res. 6:183-195(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12644670; DOI=10.1104/pp.013052;
RA Vernoud V., Horton A.C., Yang Z., Nielsen E.;
RT "Analysis of the small GTPase gene superfamily of Arabidopsis.";
RL Plant Physiol. 131:1191-1208(2003).
RN [6]
RP GENE FAMILY.
RX PubMed=15723828; DOI=10.1093/jxb/eri099;
RA Gebbie L.K., Burn J.E., Hocart C.H., Williamson R.E.;
RT "Genes encoding ADP-ribosylation factors in Arabidopsis thaliana; genome
RT analysis and antisense suppression.";
RL J. Exp. Bot. 56:1079-1091(2005).
RN [7]
RP FUNCTION (MICROBIAL INFECTION), DISRUPTION PHENOTYPE, GENE FAMILY, AND
RP NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=22174675; DOI=10.1371/journal.ppat.1002409;
RA Nishikiori M., Mori M., Dohi K., Okamura H., Katoh E., Naito S., Meshi T.,
RA Ishikawa M.;
RT "A host small GTP-binding protein ARL8 plays crucial roles in tobamovirus
RT RNA replication.";
RL PLoS Pathog. 7:E1002409-E1002409(2011).
CC -!- FUNCTION: May play a role in lysosome motility. May play a role in
CC chromosome segregation. {ECO:0000250|UniProtKB:Q9NVJ2}.
CC -!- FUNCTION: (Microbial infection) Component of tomato mosaic virus (ToMV)
CC RNA replication complexes. Required for tobamovirus multiplication,
CC especially for efficient negative-strand RNA synthesis and viral RNA
CC capping. {ECO:0000269|PubMed:22174675}.
CC -!- SUBUNIT: Interacts with tubulin. {ECO:0000250|UniProtKB:Q9NVJ2}.
CC -!- SUBCELLULAR LOCATION: Late endosome membrane
CC {ECO:0000250|UniProtKB:Q9NVJ2}. Lysosome membrane
CC {ECO:0000250|UniProtKB:Q9NVJ2}. Cytoplasm, cytoskeleton, spindle
CC {ECO:0000250|UniProtKB:Q9NVJ2}. Note=Localizes with microtubules at the
CC spindle mid-zone during mitosis. {ECO:0000250|UniProtKB:Q9NVJ2}.
CC -!- DISRUPTION PHENOTYPE: In the triple mutants arl8a-1 arl8b-1 arl8c-1,
CC impaired multiplication of tomato mosaic virus (ToMV).
CC {ECO:0000269|PubMed:22174675}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Arf family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB08314.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB018107; BAB08314.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED94219.1; -; Genomic_DNA.
DR EMBL; AY062658; AAL32736.1; -; mRNA.
DR EMBL; AY114643; AAM47962.1; -; mRNA.
DR EMBL; AY085271; AAM62503.1; -; mRNA.
DR RefSeq; NP_568553.1; NM_123127.4.
DR AlphaFoldDB; Q8W4C8; -.
DR SMR; Q8W4C8; -.
DR IntAct; Q8W4C8; 2.
DR STRING; 3702.AT5G37680.1; -.
DR PaxDb; Q8W4C8; -.
DR PRIDE; Q8W4C8; -.
DR ProteomicsDB; 246669; -.
DR EnsemblPlants; AT5G37680.1; AT5G37680.1; AT5G37680.
DR GeneID; 833747; -.
DR Gramene; AT5G37680.1; AT5G37680.1; AT5G37680.
DR KEGG; ath:AT5G37680; -.
DR Araport; AT5G37680; -.
DR TAIR; locus:2151734; AT5G37680.
DR eggNOG; KOG0075; Eukaryota.
DR HOGENOM; CLU_040729_10_1_1; -.
DR InParanoid; Q8W4C8; -.
DR OMA; KIDIQPH; -.
DR OrthoDB; 1123043at2759; -.
DR PhylomeDB; Q8W4C8; -.
DR PRO; PR:Q8W4C8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8W4C8; baseline and differential.
DR GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; ISS:TAIR.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
DR GO; GO:0051607; P:defense response to virus; IGI:TAIR.
DR GO; GO:0015031; P:protein transport; IEA:InterPro.
DR CDD; cd04159; Arl10_like; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR044154; Arl8a/8b.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR006689; Small_GTPase_ARF/SAR.
DR Pfam; PF00025; Arf; 1.
DR PRINTS; PR00328; SAR1GTPBP.
DR SMART; SM00178; SAR; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51417; ARF; 1.
PE 2: Evidence at transcript level;
KW Cell cycle; Cell division; Chromosome partition; Cytoplasm; Cytoskeleton;
KW Endosome; GTP-binding; Lysosome; Membrane; Mitosis; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..184
FT /note="ADP-ribosylation factor-like protein 8c"
FT /id="PRO_0000438005"
FT INTRAMEM 1..18
FT /note="Note=Mediates targeting to membranes"
FT /evidence="ECO:0000250|UniProtKB:Q9NVJ2"
FT BINDING 29..34
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P62330"
FT BINDING 48..51
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P62330"
FT BINDING 70..74
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P62330"
FT BINDING 129..132
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P62330"
SQ SEQUENCE 184 AA; 20573 MW; D3EE2D625E8C94CC CRC64;
MGLWDSLLNW LRSLFFKQEM ELSLVGLQNA GKTSLVNAIA TGGYSEDMIP TVGFNMRKVT
KGNVTIKIWD LGGQRRFRTM WERYCRGVSA IVYVIDAADR DSVPISRSEL NDLLTKPSLN
GIPLLILGNK IDKSEALSKQ ALVDQLGLES VTDREVCCYM ISCKDSINID AVIDWLIKHS
RTAT
