ID   METK_MAIZE              Reviewed;          15 AA.
AC   P80616;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   23-FEB-2022, entry version 75.
DE   RecName: Full=Probable S-adenosylmethionine synthase;
DE            Short=AdoMet synthase;
DE            EC=2.5.1.6 {ECO:0000250|UniProtKB:Q96551};
DE   AltName: Full=Methionine adenosyltransferase;
DE            Short=MAT;
DE   AltName: Full=Spot 178;
DE   Flags: Fragment;
GN   Name=METK;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Coleoptile;
RX   AGRICOLA=IND20551642; DOI=10.1007/BF00224104;
RA   Touzet P., Riccardi F., Morin C., Damerval C., Huet J.-C., Pernollet J.-C.,
RA   Zivy M., de Vienne D.;
RT   "The maize two dimensional gel protein database: towards an integrated
RT   genome analysis program.";
RL   Theor. Appl. Genet. 93:997-1005(1996).
CC   -!- FUNCTION: Catalyzes the formation of S-adenosylmethionine from
CC       methionine and ATP. The reaction comprises two steps that are both
CC       catalyzed by the same enzyme: formation of S-adenosylmethionine
CC       (AdoMet) and triphosphate, and subsequent hydrolysis of the
CC       triphosphate. {ECO:0000250|UniProtKB:Q96551}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + L-methionine = diphosphate + phosphate + S-
CC         adenosyl-L-methionine; Xref=Rhea:RHEA:21080, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57844, ChEBI:CHEBI:59789; EC=2.5.1.6;
CC         Evidence={ECO:0000250|UniProtKB:Q96551};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000250|UniProtKB:Q96551};
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000250|UniProtKB:Q96551};
CC       Name=Co(2+); Xref=ChEBI:CHEBI:48828;
CC         Evidence={ECO:0000250|UniProtKB:Q96551};
CC       Note=Binds 2 divalent ions per subunit. The metal ions interact
CC       primarily with the substrate (By similarity). Can utilize magnesium,
CC       manganese or cobalt (in vitro) (By similarity).
CC       {ECO:0000250|UniProtKB:P13444, ECO:0000250|UniProtKB:Q96551};
CC   -!- COFACTOR:
CC       Name=K(+); Xref=ChEBI:CHEBI:29103;
CC         Evidence={ECO:0000250|UniProtKB:Q96551};
CC       Note=Binds 1 potassium ion per subunit. The potassium ion interacts
CC       primarily with the substrate (By similarity).
CC       {ECO:0000250|UniProtKB:P13444};
CC   -!- PATHWAY: Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis;
CC       S-adenosyl-L-methionine from L-methionine: step 1/1.
CC       {ECO:0000250|UniProtKB:Q96551}.
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- MISCELLANEOUS: On the 2D-gel the determined pI of this unknown protein
CC       is: 5.9, its MW is: 43.3 kDa.
CC   -!- SIMILARITY: Belongs to the AdoMet synthase family. {ECO:0000305}.
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DR   MaizeGDB; 123938; -.
DR   eggNOG; KOG1506; Eukaryota.
DR   UniPathway; UPA00315; UER00080.
DR   Proteomes; UP000007305; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004478; F:methionine adenosyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0006556; P:S-adenosylmethionine biosynthetic process; IEA:UniProtKB-UniPathway.
PE   1: Evidence at protein level;
KW   ATP-binding; Cobalt; Cytoplasm; Direct protein sequencing; Magnesium;
KW   Metal-binding; Nucleotide-binding; One-carbon metabolism; Potassium;
KW   Reference proteome; Transferase.
FT   CHAIN           <1..>15
FT                   /note="Probable S-adenosylmethionine synthase"
FT                   /id="PRO_0000174467"
FT   NON_TER         1
FT   NON_TER         15
SQ   SEQUENCE   15 AA;  1601 MW;  232B07E9AC328D32 CRC64;
     RPEEIGAGDQ GHMFG