ID   ARLS_STAES              Reviewed;         456 AA.
AC   Q8CSL7;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 114.
DE   RecName: Full=Signal transduction histidine-protein kinase ArlS;
DE            EC=2.7.13.3;
GN   Name=arlS; OrderedLocusNames=SE_1099;
OS   Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=176280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 12228 / FDA PCI 1200;
RX   PubMed=12950922; DOI=10.1046/j.1365-2958.2003.03671.x;
RA   Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q.,
RA   Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H.,
RA   Zhao G.-P., Qu D., Danchin A., Wen Y.-M.;
RT   "Genome-based analysis of virulence genes in a non-biofilm-forming
RT   Staphylococcus epidermidis strain (ATCC 12228).";
RL   Mol. Microbiol. 49:1577-1593(2003).
CC   -!- FUNCTION: Member of the two-component regulatory system ArlS/ArlR. ArlS
CC       probably functions as a sensor protein kinase which is
CC       autophosphorylated at a histidine residue and transfers its phosphate
CC       group to ArlR (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC         histidine.; EC=2.7.13.3;
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000250}.
CC   -!- PTM: Autophosphorylated. {ECO:0000250}.
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DR   EMBL; AE015929; AAO04696.1; -; Genomic_DNA.
DR   RefSeq; NP_764654.1; NC_004461.1.
DR   RefSeq; WP_001831043.1; NZ_WBME01000002.1.
DR   AlphaFoldDB; Q8CSL7; -.
DR   SMR; Q8CSL7; -.
DR   STRING; 176280.SE_1099; -.
DR   EnsemblBacteria; AAO04696; AAO04696; SE_1099.
DR   GeneID; 50018776; -.
DR   KEGG; sep:SE_1099; -.
DR   PATRIC; fig|176280.10.peg.1074; -.
DR   eggNOG; COG5002; Bacteria.
DR   HOGENOM; CLU_000445_89_6_9; -.
DR   OMA; TVIGNFR; -.
DR   Proteomes; UP000001411; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR   CDD; cd00082; HisKA; 1.
DR   Gene3D; 3.30.565.10; -; 1.
DR   InterPro; IPR041610; ArlS_N.
DR   InterPro; IPR003660; HAMP_dom.
DR   InterPro; IPR003594; HATPase_C.
DR   InterPro; IPR036890; HATPase_C_sf.
DR   InterPro; IPR005467; His_kinase_dom.
DR   InterPro; IPR003661; HisK_dim/P.
DR   InterPro; IPR036097; HisK_dim/P_sf.
DR   InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR   Pfam; PF18719; ArlS_N; 1.
DR   Pfam; PF02518; HATPase_c; 1.
DR   Pfam; PF00512; HisKA; 1.
DR   PRINTS; PR00344; BCTRLSENSOR.
DR   SMART; SM00387; HATPase_c; 1.
DR   SMART; SM00388; HisKA; 1.
DR   SUPFAM; SSF47384; SSF47384; 1.
DR   SUPFAM; SSF55874; SSF55874; 1.
DR   PROSITE; PS50885; HAMP; 1.
DR   PROSITE; PS50109; HIS_KIN; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell membrane; Kinase; Membrane; Nucleotide-binding;
KW   Phosphoprotein; Transferase; Transmembrane; Transmembrane helix;
KW   Two-component regulatory system.
FT   CHAIN           1..456
FT                   /note="Signal transduction histidine-protein kinase ArlS"
FT                   /id="PRO_0000293449"
FT   TRANSMEM        13..33
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        157..177
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          179..232
FT                   /note="HAMP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00102"
FT   DOMAIN          240..456
FT                   /note="Histidine kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT   MOD_RES         243
FT                   /note="Phosphohistidine; by autocatalysis"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
SQ   SEQUENCE   456 AA;  52860 MW;  8ACF8A6CF9D7F77B CRC64;
     MIKRQKLKYK WMLITTLITF TTILLFCLII IFFLKDTLRS SEIDEAERSS NDIANLFHSK
     SLSDISALDL NASLENFQEI LIYDDKGRKL IQTSNDNTLA YDNKIDFKHP ERIHIQRSHG
     INYLVITEPI RSKDFSGYSV LVHSLQNYDN LVKSLYIVAL AFGLIATIIT AGVSYIFSSQ
     ITKPIVTMSN KMNQIRRDGF QNKLELTTNY EETDNLIDTF NEMMYQIEES FNQQRQFVED
     ASHELRTPLQ IIQGHLNLIQ RWGKKDPAVL EESLNISIEE VNRITKLVEE LLLLTKDRVN
     HNVLECENVD VNSEIQSRVK SLQHLHPDYT FETHLATKPI QLKINRHQFE QLLLIFIDNA
     MKYDTEHKHI KIVTQLKNKM IMIDITDHGM GIPKADLEFI FDRFYRVDKS RARSQGGNGL
     GLSIAEKIVQ LNGGMIQVES ELQKYTTFKI SFPVLN