ID   ARLS_STAS1              Reviewed;         451 AA.
AC   Q49XM6;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   25-MAY-2022, entry version 114.
DE   RecName: Full=Signal transduction histidine-protein kinase ArlS;
DE            EC=2.7.13.3;
GN   Name=arlS; OrderedLocusNames=SSP1324;
OS   Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM
OS   20229 / NCIMB 8711 / NCTC 7292 / S-41).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=342451;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41;
RX   PubMed=16135568; DOI=10.1073/pnas.0502950102;
RA   Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M.,
RA   Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.;
RT   "Whole genome sequence of Staphylococcus saprophyticus reveals the
RT   pathogenesis of uncomplicated urinary tract infection.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005).
CC   -!- FUNCTION: Member of the two-component regulatory system ArlS/ArlR. ArlS
CC       probably functions as a sensor protein kinase which is
CC       autophosphorylated at a histidine residue and transfers its phosphate
CC       group to ArlR (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC         histidine.; EC=2.7.13.3;
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000250}.
CC   -!- PTM: Autophosphorylated. {ECO:0000250}.
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DR   EMBL; AP008934; BAE18469.1; -; Genomic_DNA.
DR   RefSeq; WP_011303105.1; NZ_MTGA01000038.1.
DR   AlphaFoldDB; Q49XM6; -.
DR   SMR; Q49XM6; -.
DR   STRING; 342451.SSP1324; -.
DR   EnsemblBacteria; BAE18469; BAE18469; SSP1324.
DR   KEGG; ssp:SSP1324; -.
DR   PATRIC; fig|342451.11.peg.1327; -.
DR   eggNOG; COG5002; Bacteria.
DR   HOGENOM; CLU_000445_89_6_9; -.
DR   OMA; TVIGNFR; -.
DR   OrthoDB; 692375at2; -.
DR   Proteomes; UP000006371; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR   CDD; cd00082; HisKA; 1.
DR   Gene3D; 3.30.565.10; -; 1.
DR   InterPro; IPR041610; ArlS_N.
DR   InterPro; IPR003660; HAMP_dom.
DR   InterPro; IPR003594; HATPase_C.
DR   InterPro; IPR036890; HATPase_C_sf.
DR   InterPro; IPR005467; His_kinase_dom.
DR   InterPro; IPR003661; HisK_dim/P.
DR   InterPro; IPR036097; HisK_dim/P_sf.
DR   InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR   Pfam; PF18719; ArlS_N; 1.
DR   Pfam; PF02518; HATPase_c; 1.
DR   Pfam; PF00512; HisKA; 1.
DR   PRINTS; PR00344; BCTRLSENSOR.
DR   SMART; SM00387; HATPase_c; 1.
DR   SMART; SM00388; HisKA; 1.
DR   SUPFAM; SSF47384; SSF47384; 1.
DR   SUPFAM; SSF55874; SSF55874; 1.
DR   PROSITE; PS50885; HAMP; 1.
DR   PROSITE; PS50109; HIS_KIN; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell membrane; Kinase; Membrane; Nucleotide-binding;
KW   Phosphoprotein; Reference proteome; Transferase; Transmembrane;
KW   Transmembrane helix; Two-component regulatory system.
FT   CHAIN           1..451
FT                   /note="Signal transduction histidine-protein kinase ArlS"
FT                   /id="PRO_0000293452"
FT   TRANSMEM        12..32
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        156..176
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          178..231
FT                   /note="HAMP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00102"
FT   DOMAIN          239..451
FT                   /note="Histidine kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT   MOD_RES         242
FT                   /note="Phosphohistidine; by autocatalysis"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
SQ   SEQUENCE   451 AA;  52423 MW;  0750F2CF493D1AC8 CRC64;
     MKQRKLKTKW MLITTTITFL TIFLFSIIII FFLSNSLRHN EVDEAERSSE DIVKLFESKQ
     IEHVTPLDLN ASLGNFQKVM LFNEDGHKLM ETSNDNSITF SPNIVPTNVN RIAVKSDHKK
     DYLIITDHIE SPKFNGYSVI VHSLEDYKAL VNSLYFIALI FGVIATFITA IISYFFSSQI
     TKPLILMSNK MQQIRRDGFQ EKVELSTNYE ETDNLIVTFN EMMLQLEESF NQQRQFVEDA
     SHELRTPLQI IQGHLNLINR WGKKDAAILE ESLDISLEEM TRITKLVEEL LLLTKDNNNS
     RDGEIENVEI NQEIASRIKS LSQLHSDYTF EFDAFPKPLN IKIDRYQFEQ MLIIFIDNAM
     KYDQINKYIQ IQTKLRNKQI SIEITDHGVG IPKEDIEFIF DRFYRVDKSR SRKLGGNGLG
     LSIAKKIIEL NNGTIHVDSE VDKYTTFKIT F