ID   ARLY2_AGRFC             Reviewed;         504 AA.
AC   Q8U705;
DT   23-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=Argininosuccinate lyase 2 {ECO:0000255|HAMAP-Rule:MF_00006};
DE            Short=ASAL 2 {ECO:0000255|HAMAP-Rule:MF_00006};
DE            EC=4.3.2.1 {ECO:0000255|HAMAP-Rule:MF_00006};
DE   AltName: Full=Arginosuccinase {ECO:0000255|HAMAP-Rule:MF_00006};
GN   Name=argH2 {ECO:0000255|HAMAP-Rule:MF_00006}; OrderedLocusNames=Atu4651;
GN   ORFNames=AGR_L_463;
OS   Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS   (strain C58)).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=176299;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743193; DOI=10.1126/science.1066804;
RA   Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA   Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA   Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA   Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA   Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA   Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA   Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA   Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA   Gordon M.P., Olson M.V., Nester E.W.;
RT   "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT   C58.";
RL   Science 294:2317-2323(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743194; DOI=10.1126/science.1066803;
RA   Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA   Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA   Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA   Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA   Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT   "Genome sequence of the plant pathogen and biotechnology agent
RT   Agrobacterium tumefaciens C58.";
RL   Science 294:2323-2328(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-(N(omega)-L-arginino)succinate = fumarate + L-arginine;
CC         Xref=Rhea:RHEA:24020, ChEBI:CHEBI:29806, ChEBI:CHEBI:32682,
CC         ChEBI:CHEBI:57472; EC=4.3.2.1; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00006};
CC   -!- PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine
CC       from L-ornithine and carbamoyl phosphate: step 3/3. {ECO:0000255|HAMAP-
CC       Rule:MF_00006}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00006}.
CC   -!- SIMILARITY: Belongs to the lyase 1 family. Argininosuccinate lyase
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00006}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK88796.2; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE007870; AAK88796.2; ALT_INIT; Genomic_DNA.
DR   PIR; AG3128; AG3128.
DR   PIR; B98159; B98159.
DR   RefSeq; NP_356011.2; NC_003063.2.
DR   RefSeq; WP_035257217.1; NC_003063.2.
DR   AlphaFoldDB; Q8U705; -.
DR   SMR; Q8U705; -.
DR   STRING; 176299.Atu4651; -.
DR   EnsemblBacteria; AAK88796; AAK88796; Atu4651.
DR   KEGG; atu:Atu4651; -.
DR   PATRIC; fig|176299.10.peg.4456; -.
DR   eggNOG; COG0165; Bacteria.
DR   HOGENOM; CLU_027272_3_0_5; -.
DR   OMA; YTNGVAA; -.
DR   BioCyc; AGRO:ATU4651-MON; -.
DR   UniPathway; UPA00068; UER00114.
DR   Proteomes; UP000000813; Chromosome linear.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004056; F:argininosuccinate lyase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0042450; P:arginine biosynthetic process via ornithine; IEA:InterPro.
DR   CDD; cd01359; Argininosuccinate_lyase; 1.
DR   Gene3D; 1.10.275.10; -; 1.
DR   HAMAP; MF_00006; Arg_succ_lyase; 1.
DR   InterPro; IPR029419; Arg_succ_lyase_C.
DR   InterPro; IPR009049; Argininosuccinate_lyase.
DR   InterPro; IPR024083; Fumarase/histidase_N.
DR   InterPro; IPR000362; Fumarate_lyase_fam.
DR   InterPro; IPR022761; Fumarate_lyase_N.
DR   InterPro; IPR008948; L-Aspartase-like.
DR   PANTHER; PTHR43814; PTHR43814; 1.
DR   Pfam; PF14698; ASL_C2; 1.
DR   Pfam; PF00206; Lyase_1; 1.
DR   PRINTS; PR00149; FUMRATELYASE.
DR   SUPFAM; SSF48557; SSF48557; 1.
DR   TIGRFAMs; TIGR00838; argH; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Arginine biosynthesis; Cytoplasm; Lyase;
KW   Reference proteome.
FT   CHAIN           1..504
FT                   /note="Argininosuccinate lyase 2"
FT                   /id="PRO_0000137731"
SQ   SEQUENCE   504 AA;  55699 MW;  2BBD2A090791CFB8 CRC64;
     MADINPRQSD TSKFPDPVYK ETVLRPLFDG AKNHHVDGFR RIDRAHLVML RETGILDAET
     AAKIAGALED IDRTIEPSEL VYTGEVEDFF FLIEKELKAR IGVDVAGRLH TARSRNDIDH
     TLFKIGLKDK IDTLTAKARV LLKALIDAAE RNQSTLIVAY THGQPAQPTT FGHYLSAAIE
     VLIRDIERFT EARHIVDLSP MGAAAITTSG FPIDRARVAE LLGFAAPLRN SYSCIAAVDY
     TTATYGAIEL MFLHLGRLIQ DFQFWTSFEV GQIYVPNSLV QISSIMPQKR NPVPIEHLRH
     LASQTFGRAR TMLDVMHNTP FTDMNDSEGE TQSMGYEAFA SAGRVLDLLA SLVGQISIDP
     ERVDQNIRRS CITITELADS LVRIEDLSFR QAHEIAATVA KSVVALKGDL PNDGYQPFLG
     AFSGLTGRET GIDEEKFRQI VSPEHFVAVR SRFGGPAPEP MREAFAAYRG KLGAFEAEAQ
     RSTNHEAAKA AELAEKFTAL TGAR