ID   ARLY2_PSEPF             Reviewed;         464 AA.
AC   Q3K4S8;
DT   27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=Argininosuccinate lyase 2 {ECO:0000255|HAMAP-Rule:MF_00006};
DE            Short=ASAL 2 {ECO:0000255|HAMAP-Rule:MF_00006};
DE            EC=4.3.2.1 {ECO:0000255|HAMAP-Rule:MF_00006};
DE   AltName: Full=Arginosuccinase 2 {ECO:0000255|HAMAP-Rule:MF_00006};
GN   Name=argH2 {ECO:0000255|HAMAP-Rule:MF_00006}; OrderedLocusNames=Pfl01_5489;
OS   Pseudomonas fluorescens (strain Pf0-1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=205922;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Pf0-1;
RX   PubMed=19432983; DOI=10.1186/gb-2009-10-5-r51;
RA   Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R.,
RA   Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M.,
RA   Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J.,
RA   Harris S., Challis G.L., Yaxley A.M., Harris D., Seeger K., Murphy L.,
RA   Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K.,
RA   Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M.,
RA   Parkhill J., Levy S.B., Rainey P.B., Thomson N.R.;
RT   "Genomic and genetic analyses of diversity and plant interactions of
RT   Pseudomonas fluorescens.";
RL   Genome Biol. 10:R51.1-R51.16(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-(N(omega)-L-arginino)succinate = fumarate + L-arginine;
CC         Xref=Rhea:RHEA:24020, ChEBI:CHEBI:29806, ChEBI:CHEBI:32682,
CC         ChEBI:CHEBI:57472; EC=4.3.2.1; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00006};
CC   -!- PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine
CC       from L-ornithine and carbamoyl phosphate: step 3/3. {ECO:0000255|HAMAP-
CC       Rule:MF_00006}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00006}.
CC   -!- SIMILARITY: Belongs to the lyase 1 family. Argininosuccinate lyase
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00006}.
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DR   EMBL; CP000094; ABA77226.1; -; Genomic_DNA.
DR   RefSeq; WP_011336517.1; NC_007492.2.
DR   AlphaFoldDB; Q3K4S8; -.
DR   SMR; Q3K4S8; -.
DR   STRING; 205922.Pfl01_5489; -.
DR   PRIDE; Q3K4S8; -.
DR   EnsemblBacteria; ABA77226; ABA77226; Pfl01_5489.
DR   KEGG; pfo:Pfl01_5489; -.
DR   eggNOG; COG0165; Bacteria.
DR   HOGENOM; CLU_027272_2_3_6; -.
DR   OMA; KKNPDVF; -.
DR   UniPathway; UPA00068; UER00114.
DR   Proteomes; UP000002704; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004056; F:argininosuccinate lyase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0042450; P:arginine biosynthetic process via ornithine; IEA:InterPro.
DR   CDD; cd01359; Argininosuccinate_lyase; 1.
DR   Gene3D; 1.10.275.10; -; 1.
DR   HAMAP; MF_00006; Arg_succ_lyase; 1.
DR   InterPro; IPR029419; Arg_succ_lyase_C.
DR   InterPro; IPR009049; Argininosuccinate_lyase.
DR   InterPro; IPR024083; Fumarase/histidase_N.
DR   InterPro; IPR020557; Fumarate_lyase_CS.
DR   InterPro; IPR000362; Fumarate_lyase_fam.
DR   InterPro; IPR022761; Fumarate_lyase_N.
DR   InterPro; IPR008948; L-Aspartase-like.
DR   PANTHER; PTHR43814; PTHR43814; 1.
DR   Pfam; PF14698; ASL_C2; 1.
DR   Pfam; PF00206; Lyase_1; 1.
DR   PRINTS; PR00149; FUMRATELYASE.
DR   SUPFAM; SSF48557; SSF48557; 1.
DR   TIGRFAMs; TIGR00838; argH; 1.
DR   PROSITE; PS00163; FUMARATE_LYASES; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Arginine biosynthesis; Cytoplasm; Lyase.
FT   CHAIN           1..464
FT                   /note="Argininosuccinate lyase 2"
FT                   /id="PRO_0000240753"
SQ   SEQUENCE   464 AA;  51682 MW;  AC00BE9F81D97D64 CRC64;
     MSTDKTNQSW GGRFSEPVDA FVARFTASVT FDQRLYRHDI MGSIAHATML AKVGVLTDAE
     RDSIIDGLKT IQGEIEAGQF DWRVDLEDVH MNIEARLTDR IGVTGKKLHT GRSRNDQVAT
     DIRLWLRDEI DLILSEITRL QKGLLEQAER EAASIMPGFT HLQTAQPVTF GHHMLAWFEM
     LSRDYERLVD CRKRTNRMPL GSAALAGTTY PIDREYTAQL LGFDAVGGNS LDNVSDRDFA
     IEFCSAASIA MMHLSRFSEE LVLWTSAQFQ FIDLPDRFCT GSSIMPQKKN PDVPELVRGK
     TGRVFGALMG LLTLMKGQPL AYNKDNQEDK EPLFDAADTL RDSLRAFADM IPAIKPKHAI
     MREAALRGFS TATDLADYLV RRGLPFRDCH EIVGHAVKYG VDTGKDLAEM SLEELRQFSD
     QIEQDVFAVL TLEGSVNARD HIGGTAPAQV KAAVVRGQAL IASR