METL_DROME
ID METL_DROME Reviewed; 325 AA.
AC Q86BS6; Q960S9; Q9W0C0;
DT 03-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2003, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Methyltransferase-like protein;
DE EC=2.1.1.-;
GN Name=metl; ORFNames=CG13929;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP ALTERNATIVE SPLICING (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, AND
RP INTERACTION WITH PSN.
RX PubMed=11738826; DOI=10.1016/s0378-1119(01)00770-3;
RA Zhang S.X., Guo Y., Boulianne G.L.;
RT "Identification of a novel family of putative methyltransferases that
RT interact with human and Drosophila presenilins.";
RL Gene 280:135-144(2001).
CC -!- FUNCTION: Probable methyltransferase.
CC -!- SUBUNIT: Interacts with Psn. {ECO:0000269|PubMed:11738826}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1; Synonyms=B;
CC IsoId=Q86BS6-1; Sequence=Displayed;
CC Name=2; Synonyms=Metl-a;
CC IsoId=Q86BS6-2; Sequence=VSP_008484;
CC Name=3; Synonyms=A;
CC IsoId=Q86BS6-3; Sequence=VSP_008483;
CC -!- TISSUE SPECIFICITY: Widely expressed. Expressed in ovaries, head,
CC thorax and abdomen of adult flies, and in the CNS of third instar
CC larvae. Isoform 2 is predominantly expressed in larvae and in adult
CC tissues that have been tested. {ECO:0000269|PubMed:11738826}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout embryogenesis, as well as in
CC larvae and adults.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. METL family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE014296; AAF47531.2; -; Genomic_DNA.
DR EMBL; AE014296; AAF47532.2; -; Genomic_DNA.
DR EMBL; AY051875; AAK93299.1; -; mRNA.
DR RefSeq; NP_647636.3; NM_139379.4. [Q86BS6-1]
DR RefSeq; NP_728647.2; NM_167907.3. [Q86BS6-3]
DR AlphaFoldDB; Q86BS6; -.
DR SMR; Q86BS6; -.
DR BioGRID; 63731; 8.
DR IntAct; Q86BS6; 9.
DR STRING; 7227.FBpp0072696; -.
DR PaxDb; Q86BS6; -.
DR PRIDE; Q86BS6; -.
DR DNASU; 38197; -.
DR EnsemblMetazoa; FBtr0072813; FBpp0072695; FBgn0035247. [Q86BS6-3]
DR EnsemblMetazoa; FBtr0072814; FBpp0072696; FBgn0035247. [Q86BS6-1]
DR GeneID; 38197; -.
DR KEGG; dme:Dmel_CG13929; -.
DR UCSC; CG13929-RA; d. melanogaster. [Q86BS6-1]
DR CTD; 38197; -.
DR FlyBase; FBgn0035247; metl.
DR VEuPathDB; VectorBase:FBgn0035247; -.
DR eggNOG; KOG2361; Eukaryota.
DR GeneTree; ENSGT00940000171213; -.
DR InParanoid; Q86BS6; -.
DR OMA; PAKWWNL; -.
DR PhylomeDB; Q86BS6; -.
DR SignaLink; Q86BS6; -.
DR BioGRID-ORCS; 38197; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 38197; -.
DR PRO; PR:Q86BS6; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0035247; Expressed in oviduct (Drosophila) and 17 other tissues.
DR ExpressionAtlas; Q86BS6; baseline and differential.
DR Genevisible; Q86BS6; DM.
DR GO; GO:0016427; F:tRNA (cytosine) methyltransferase activity; ISS:FlyBase.
DR GO; GO:0052735; F:tRNA (cytosine-3-)-methyltransferase activity; IBA:GO_Central.
DR GO; GO:0030488; P:tRNA methylation; ISS:FlyBase.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR041698; Methyltransf_25.
DR InterPro; IPR026113; MeTrfase.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR22809; PTHR22809; 1.
DR Pfam; PF13649; Methyltransf_25; 1.
DR PIRSF; PIRSF037755; Mettl2_prd; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Methyltransferase; Reference proteome; Transferase.
FT CHAIN 1..325
FT /note="Methyltransferase-like protein"
FT /id="PRO_0000204456"
FT VAR_SEQ 1..79
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_008484"
FT VAR_SEQ 1..52
FT /note="MLTTSSSTMLGKYGWQILRRLASKSWEHTRQRKKPSGAGSRVLTDAREVFEF
FT -> MSDNPAAENEKRPQFGTRLLTDADDVFKH (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_008483"
SQ SEQUENCE 325 AA; 38132 MW; 3EFBB4FDC1C7311D CRC64;
MLTTSSSTML GKYGWQILRR LASKSWEHTR QRKKPSGAGS RVLTDAREVF EFNAWDHVQW
DEEQELAAKA AVAKNSTSKM EAEQKERFQT DAPKFWDSFY GIHDNRFFKD RHWLFTEFPE
LAPLAADSAV LQPRSIFELG CGVGNTILPL LQYSSEPQLK VFGCDFSARA IEILRSQRQF
DEKRCEVFVM DATLDHWQVP FEENSQDIIV MIFVLSAIEP KKMQRVLDNC YRYLRPGGLL
LFRDYGRYDL AQLRFKSGKC MEDNFYVRGD GTMVYFFTEE ELRGMMTQAG LQEEQLIVDR
RLQVNRCRGL KMYRVWIQTK FRKPL
