METN_ERWPE
ID METN_ERWPE Reviewed; 326 AA.
AC Q8GEH7; C8ZLQ5;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Methionine import ATP-binding protein MetN {ECO:0000255|HAMAP-Rule:MF_01719};
DE EC=7.4.2.11 {ECO:0000255|HAMAP-Rule:MF_01719};
GN Name=metN {ECO:0000255|HAMAP-Rule:MF_01719};
GN OrderedLocusNames=EpC_pEp360040;
OS Erwinia pyrifoliae (strain DSM 12162 / Ep1/96).
OG Plasmid pEP36.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Erwiniaceae; Erwinia.
OX NCBI_TaxID=634499;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=DSM 12162 / Ep1/96;
RX PubMed=12450843; DOI=10.1128/aem.68.12.6182-6192.2002;
RA McGhee G.C., Schnabel E.L., Maxson-Stein K., Jones B., Stromberg V.K.,
RA Lacy G.H., Jones A.L.;
RT "Relatedness of chromosomal and plasmid DNAs of Erwinia pyrifoliae and
RT Erwinia amylovora.";
RL Appl. Environ. Microbiol. 68:6182-6192(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 12162 / Ep1/96;
RX PubMed=20565991; DOI=10.1186/1471-2164-11-393;
RA Kube M., Migdoll A.M., Gehring I., Heitmann K., Mayer Y., Kuhl H.,
RA Knaust F., Geider K., Reinhardt R.;
RT "Genome comparison of the epiphytic bacteria Erwinia billingiae and E.
RT tasmaniensis with the pear pathogen E. pyrifoliae.";
RL BMC Genomics 11:393-393(2010).
CC -!- FUNCTION: Part of the ABC transporter complex MetNIQ involved in
CC methionine import. Responsible for energy coupling to the transport
CC system. {ECO:0000255|HAMAP-Rule:MF_01719}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + L-methionine(out) = ADP + H(+) + L-methionine(in)
CC + phosphate; Xref=Rhea:RHEA:29779, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57844, ChEBI:CHEBI:456216; EC=7.4.2.11;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01719};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-methionine(out) + H2O = ADP + D-methionine(in) + H(+)
CC + phosphate; Xref=Rhea:RHEA:29767, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57932, ChEBI:CHEBI:456216; EC=7.4.2.11;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01719};
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (MetN),
CC two transmembrane proteins (MetI) and a solute-binding protein (MetQ).
CC {ECO:0000255|HAMAP-Rule:MF_01719}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01719}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01719}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Methionine
CC importer (TC 3.A.1.24) family. {ECO:0000255|HAMAP-Rule:MF_01719}.
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DR EMBL; AY123045; AAN04540.1; -; Genomic_DNA.
DR EMBL; FP236829; CAX53406.1; -; Genomic_DNA.
DR RefSeq; NP_758753.1; NC_004445.1.
DR RefSeq; WP_011078067.1; NC_013263.1.
DR AlphaFoldDB; Q8GEH7; -.
DR SMR; Q8GEH7; -.
DR KEGG; epy:EpC_pEp360040; -.
DR HOGENOM; CLU_000604_1_3_6; -.
DR OMA; ICTDVAV; -.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0033232; F:ABC-type D-methionine transporter activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR CDD; cd03258; ABC_MetN_methionine_transporter; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR045865; ACT-like_dom_sf.
DR InterPro; IPR041701; MetN_ABC.
DR InterPro; IPR018449; NIL_domain.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00005; ABC_tran; 1.
DR Pfam; PF09383; NIL; 1.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00930; NIL; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF55021; SSF55021; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR PROSITE; PS51264; METN; 1.
PE 3: Inferred from homology;
KW Amino-acid transport; ATP-binding; Cell inner membrane; Cell membrane;
KW Membrane; Nucleotide-binding; Plasmid; Translocase; Transport.
FT CHAIN 1..326
FT /note="Methionine import ATP-binding protein MetN"
FT /id="PRO_0000270297"
FT DOMAIN 1..226
FT /note="ABC transporter"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01719"
FT BINDING 23..30
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01719"
SQ SEQUENCE 326 AA; 35739 MW; 39CD99C0A8F743BF CRC64;
MVFYTIGPQT PQIDKGQIYG IIGYSGAGKS TLIRLLNGLE KPGSGSVTIA GQDIAQASVE
ALRQARLKIS MVFQHFNLLW SRTVSENIAF SLQISGTSKS VIQDRVQELI ALVGLVGKEQ
AYPSQLSGGQ KQRVGIARAL ANNPNVLLCD EATSALDPQT TDAILELLLD INRKLWLTIV
LITHEMHVVR KICHRVAVME EGRIVEEGEV LSLFTHPQQP ITRQFVKQTS GYISANVPFN
PQLVNIDNGK VLKLTFVGQS TQQPVIGELT LKYGLAFNML HGIMTQTTNG TFGEIWLQVP
ASQSQLPRIL ADLHAYEIST EVVTNV
