METN_HELPY
ID METN_HELPY Reviewed; 327 AA.
AC O26096; O30552;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Methionine import ATP-binding protein MetN {ECO:0000255|HAMAP-Rule:MF_01719};
DE EC=7.4.2.11 {ECO:0000255|HAMAP-Rule:MF_01719};
GN Name=metN {ECO:0000255|HAMAP-Rule:MF_01719}; OrderedLocusNames=HP_1576;
OS Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=85962;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 43504 / NCTC 11637 / JCM 7653 / RPH 13487;
RX PubMed=9294450; DOI=10.1128/jb.179.18.5892-5902.1997;
RA Hendricks J.K., Mobley H.L.T.;
RT "Helicobacter pylori ABC transporter: effect of allelic exchange
RT mutagenesis on urease activity.";
RL J. Bacteriol. 179:5892-5902(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700392 / 26695;
RX PubMed=9252185; DOI=10.1038/41483;
RA Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G.,
RA Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A.,
RA Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N.,
RA Loftus B.J., Richardson D.L., Dodson R.J., Khalak H.G., Glodek A.,
RA McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E.,
RA Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D.,
RA Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S.,
RA Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.;
RT "The complete genome sequence of the gastric pathogen Helicobacter
RT pylori.";
RL Nature 388:539-547(1997).
CC -!- FUNCTION: Part of the ABC transporter complex MetNIQ involved in
CC methionine import. Responsible for energy coupling to the transport
CC system. {ECO:0000255|HAMAP-Rule:MF_01719}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + L-methionine(out) = ADP + H(+) + L-methionine(in)
CC + phosphate; Xref=Rhea:RHEA:29779, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57844, ChEBI:CHEBI:456216; EC=7.4.2.11;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01719};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-methionine(out) + H2O = ADP + D-methionine(in) + H(+)
CC + phosphate; Xref=Rhea:RHEA:29767, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57932, ChEBI:CHEBI:456216; EC=7.4.2.11;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01719};
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (MetN),
CC two transmembrane proteins (MetI) and a solute-binding protein (MetQ).
CC {ECO:0000255|HAMAP-Rule:MF_01719}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01719}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01719}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Methionine
CC importer (TC 3.A.1.24) family. {ECO:0000255|HAMAP-Rule:MF_01719}.
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DR EMBL; AF010307; AAC45666.1; -; Genomic_DNA.
DR EMBL; AE000511; AAD08616.1; -; Genomic_DNA.
DR PIR; H64716; H64716.
DR RefSeq; NP_208367.1; NC_000915.1.
DR RefSeq; WP_000259757.1; NC_018939.1.
DR AlphaFoldDB; O26096; -.
DR SMR; O26096; -.
DR DIP; DIP-3488N; -.
DR IntAct; O26096; 3.
DR MINT; O26096; -.
DR STRING; 85962.C694_08165; -.
DR PaxDb; O26096; -.
DR EnsemblBacteria; AAD08616; AAD08616; HP_1576.
DR KEGG; hpy:HP_1576; -.
DR PATRIC; fig|85962.47.peg.1694; -.
DR eggNOG; COG1135; Bacteria.
DR OMA; VFITHEI; -.
DR PhylomeDB; O26096; -.
DR Proteomes; UP000000429; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0033232; F:ABC-type D-methionine transporter activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR CDD; cd03258; ABC_MetN_methionine_transporter; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR026253; ABC_MetN.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR045865; ACT-like_dom_sf.
DR InterPro; IPR041701; MetN_ABC.
DR InterPro; IPR018449; NIL_domain.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR43166:SF5; PTHR43166:SF5; 1.
DR Pfam; PF00005; ABC_tran; 1.
DR Pfam; PF09383; NIL; 1.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00930; NIL; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF55021; SSF55021; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR PROSITE; PS51264; METN; 1.
PE 3: Inferred from homology;
KW Amino-acid transport; ATP-binding; Cell inner membrane; Cell membrane;
KW Membrane; Nucleotide-binding; Reference proteome; Translocase; Transport.
FT CHAIN 1..327
FT /note="Methionine import ATP-binding protein MetN"
FT /id="PRO_0000270310"
FT DOMAIN 3..239
FT /note="ABC transporter"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01719"
FT BINDING 36..43
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01719"
FT CONFLICT 96
FT /note="N -> D (in Ref. 1; AAC45666)"
FT /evidence="ECO:0000305"
FT CONFLICT 113
FT /note="N -> T (in Ref. 1; AAC45666)"
FT /evidence="ECO:0000305"
FT CONFLICT 132
FT /note="M -> V (in Ref. 1; AAC45666)"
FT /evidence="ECO:0000305"
FT CONFLICT 145
FT /note="Q -> E (in Ref. 1; AAC45666)"
FT /evidence="ECO:0000305"
FT CONFLICT 192
FT /note="I -> V (in Ref. 1; AAC45666)"
FT /evidence="ECO:0000305"
FT CONFLICT 198
FT /note="E -> Q (in Ref. 1; AAC45666)"
FT /evidence="ECO:0000305"
FT CONFLICT 247
FT /note="D -> N (in Ref. 1; AAC45666)"
FT /evidence="ECO:0000305"
FT CONFLICT 279
FT /note="V -> M (in Ref. 1; AAC45666)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 327 AA; 36622 MW; 89F50E5A8D7D1E7B CRC64;
MVVELKNIEK IYENGFHALK GVNLELKKGD ILGVIGYSGA GKSTLIRLIN CLERPSSGEV
LVNGVNLLKL KPKELQKARQ KIGMIFQHFN LLSAKNVFEN VAFALEIARW EKNKIKSRVH
ELLELVGLED KMHFYPKQLS GGQKQRVAIA RSLANCPDLL LCDEATSALD SKTTHSILTL
LSGIQKKLDL SIVFITHEIE VVKELCNQMC VISSGEIVER GSVEEIFANP KHAVTKELLG
IRNEHADKKS QDVYRIVFLG EHLDEPIISN LIKRFKIDVS IISGNIEELT TKDIGYLVVR
FLGNTAETQR ALEYLNALGL QVEKLKD
