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METQ_ECOLI
ID   METQ_ECOLI              Reviewed;         271 AA.
AC   P28635;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 2.
DT   03-AUG-2022, entry version 170.
DE   RecName: Full=D-methionine-binding lipoprotein MetQ;
DE   Flags: Precursor;
GN   Name=metQ; Synonyms=yaeC; OrderedLocusNames=b0197, JW0193;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RA   Miyamoto K.;
RT   "Nucleotide sequence of 5' flanking region of the ribosomal RNA gene (rrnH)
RT   in E. coli.";
RL   Submitted (APR-1993) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RA   Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T.,
RA   Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S.,
RA   Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.;
RT   "Systematic sequencing of the Escherichia coli genome: analysis of the 4.0
RT   - 6.0 min (189,987 - 281,416bp) region.";
RL   Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RA   Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA   Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA   Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT   "Sequence of minutes 4-25 of Escherichia coli.";
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 127-271.
RC   STRAIN=K12;
RX   PubMed=1459951; DOI=10.1128/jb.174.24.8016-8022.1992;
RA   Gervais F.G., Drapeau G.R.;
RT   "Identification, cloning, and characterization of rcsF, a new regulator
RT   gene for exopolysaccharide synthesis that suppresses the division mutation
RT   ftsZ84 in Escherichia coli K-12.";
RL   J. Bacteriol. 174:8016-8022(1992).
RN   [7]
RP   FUNCTION.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=12169620; DOI=10.1128/jb.184.17.4930-4932.2002;
RA   Gal J., Szvetnik A., Schnell R., Kalman M.;
RT   "The metD D-methionine transporter locus of Escherichia coli is an ABC
RT   transporter gene cluster.";
RL   J. Bacteriol. 184:4930-4932(2002).
CC   -!- FUNCTION: This protein is a component of a D-methionine permease, a
CC       binding protein-dependent, ATP-driven transport system.
CC       {ECO:0000269|PubMed:12169620}.
CC   -!- INTERACTION:
CC       P28635; P0AAF3: araG; NbExp=3; IntAct=EBI-1114713, EBI-559586;
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000305}.
CC   -!- MISCELLANEOUS: The MetNIQ system is also to be able to transport the
CC       toxic methionine analog alpha-methyl-methionine.
CC   -!- SIMILARITY: Belongs to the NlpA lipoprotein family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA24507.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; D15061; BAA03657.1; -; Genomic_DNA.
DR   EMBL; U70214; AAB08625.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC73308.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA77874.1; -; Genomic_DNA.
DR   EMBL; L04474; AAA24507.1; ALT_FRAME; Genomic_DNA.
DR   PIR; E64744; E64744.
DR   RefSeq; NP_414739.1; NC_000913.3.
DR   RefSeq; WP_000874226.1; NZ_SSZK01000004.1.
DR   PDB; 4YAH; X-ray; 1.60 A; X=1-271.
DR   PDB; 6CVL; X-ray; 2.95 A; E=34-259.
DR   PDBsum; 4YAH; -.
DR   PDBsum; 6CVL; -.
DR   AlphaFoldDB; P28635; -.
DR   SMR; P28635; -.
DR   BioGRID; 4263377; 24.
DR   BioGRID; 849293; 1.
DR   ComplexPortal; CPX-2114; Methionine ABC transporter complex.
DR   DIP; DIP-11196N; -.
DR   IntAct; P28635; 10.
DR   STRING; 511145.b0197; -.
DR   TCDB; 3.A.1.24.1; the atp-binding cassette (abc) superfamily.
DR   SWISS-2DPAGE; P28635; -.
DR   jPOST; P28635; -.
DR   PaxDb; P28635; -.
DR   PRIDE; P28635; -.
DR   EnsemblBacteria; AAC73308; AAC73308; b0197.
DR   EnsemblBacteria; BAA77874; BAA77874; BAA77874.
DR   GeneID; 67416274; -.
DR   GeneID; 944893; -.
DR   KEGG; ecj:JW0193; -.
DR   KEGG; eco:b0197; -.
DR   PATRIC; fig|1411691.4.peg.2081; -.
DR   EchoBASE; EB1467; -.
DR   eggNOG; COG1464; Bacteria.
DR   HOGENOM; CLU_067080_0_0_6; -.
DR   InParanoid; P28635; -.
DR   OMA; YQDDAES; -.
DR   PhylomeDB; P28635; -.
DR   BioCyc; EcoCyc:METQ-MON; -.
DR   BioCyc; MetaCyc:METQ-MON; -.
DR   PRO; PR:P28635; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0046658; C:anchored component of plasma membrane; TAS:EcoCyc.
DR   GO; GO:0055052; C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing; IDA:EcoCyc.
DR   GO; GO:0016020; C:membrane; IDA:ComplexPortal.
DR   GO; GO:1990197; C:methionine-importing ABC transporter complex; IPI:ComplexPortal.
DR   GO; GO:0030288; C:outer membrane-bounded periplasmic space; ISM:EcoCyc.
DR   GO; GO:0048473; P:D-methionine transport; IMP:CACAO.
DR   GO; GO:1903692; P:methionine import across plasma membrane; IDA:ComplexPortal.
DR   InterPro; IPR004872; Lipoprotein_NlpA.
DR   PANTHER; PTHR30429; PTHR30429; 1.
DR   Pfam; PF03180; Lipoprotein_9; 1.
DR   PIRSF; PIRSF002854; MetQ; 1.
DR   TIGRFAMs; TIGR00363; TIGR00363; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Amino-acid transport; Cell membrane; Lipoprotein; Membrane;
KW   Palmitate; Reference proteome; Signal; Transport.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           23..271
FT                   /note="D-methionine-binding lipoprotein MetQ"
FT                   /id="PRO_0000019739"
FT   LIPID           23
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           23
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CONFLICT        200
FT                   /note="V -> L (in Ref. 6; AAA24507)"
FT                   /evidence="ECO:0000305"
FT   STRAND          32..38
FT                   /evidence="ECO:0007829|PDB:4YAH"
FT   HELIX           41..57
FT                   /evidence="ECO:0007829|PDB:4YAH"
FT   STRAND          60..70
FT                   /evidence="ECO:0007829|PDB:4YAH"
FT   HELIX           71..77
FT                   /evidence="ECO:0007829|PDB:4YAH"
FT   STRAND          80..88
FT                   /evidence="ECO:0007829|PDB:4YAH"
FT   HELIX           89..99
FT                   /evidence="ECO:0007829|PDB:4YAH"
FT   STRAND          103..108
FT                   /evidence="ECO:0007829|PDB:4YAH"
FT   STRAND          115..117
FT                   /evidence="ECO:0007829|PDB:4YAH"
FT   HELIX           124..126
FT                   /evidence="ECO:0007829|PDB:4YAH"
FT   STRAND          132..136
FT                   /evidence="ECO:0007829|PDB:4YAH"
FT   HELIX           139..151
FT                   /evidence="ECO:0007829|PDB:4YAH"
FT   HELIX           167..169
FT                   /evidence="ECO:0007829|PDB:4YAH"
FT   STRAND          170..172
FT                   /evidence="ECO:0007829|PDB:4YAH"
FT   STRAND          178..182
FT                   /evidence="ECO:0007829|PDB:4YAH"
FT   HELIX           184..186
FT                   /evidence="ECO:0007829|PDB:4YAH"
FT   HELIX           187..190
FT                   /evidence="ECO:0007829|PDB:4YAH"
FT   STRAND          196..201
FT                   /evidence="ECO:0007829|PDB:4YAH"
FT   HELIX           203..206
FT                   /evidence="ECO:0007829|PDB:4YAH"
FT   HELIX           207..209
FT                   /evidence="ECO:0007829|PDB:4YAH"
FT   HELIX           213..216
FT                   /evidence="ECO:0007829|PDB:4YAH"
FT   STRAND          218..220
FT                   /evidence="ECO:0007829|PDB:4YAH"
FT   STRAND          229..234
FT                   /evidence="ECO:0007829|PDB:4YAH"
FT   HELIX           235..237
FT                   /evidence="ECO:0007829|PDB:4YAH"
FT   HELIX           241..250
FT                   /evidence="ECO:0007829|PDB:4YAH"
FT   HELIX           253..262
FT                   /evidence="ECO:0007829|PDB:4YAH"
FT   TURN            263..265
FT                   /evidence="ECO:0007829|PDB:4YAH"
SQ   SEQUENCE   271 AA;  29432 MW;  B50CBC6FB5CD2BF7 CRC64;
     MAFKFKTFAA VGALIGSLAL VGCGQDEKDP NHIKVGVIVG AEQQVAEVAQ KVAKDKYGLD
     VELVTFNDYV LPNEALSKGD IDANAFQHKP YLDQQLKDRG YKLVAVGNTF VYPIAGYSKK
     IKSLDELQDG SQVAVPNDPT NLGRSLLLLQ KVGLIKLKDG VGLLPTVLDV VENPKNLKIV
     ELEAPQLPRS LDDAQIALAV INTTYASQIG LTPAKDGIFV EDKESPYVNL IVTREDNKDA
     ENVKKFVQAY QSDEVYEAAN KVFNGGAVKG W
 
 
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