ID   METR_ECO57              Reviewed;         317 AA.
AC   P0A9G0; P19797;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=HTH-type transcriptional regulator MetR;
GN   Name=metR; OrderedLocusNames=Z5349, ECs4758;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Control of the last step in methionine biosynthesis; MetR is
CC       a positive activator of the metA, metE and metH genes. It is also a
CC       negative regulator of its own expression (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the LysR transcriptional regulatory family.
CC       {ECO:0000305}.
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DR   EMBL; AE005174; AAG59024.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB38181.1; -; Genomic_DNA.
DR   PIR; D86070; D86070.
DR   PIR; F91223; F91223.
DR   RefSeq; NP_312785.1; NC_002695.1.
DR   RefSeq; WP_000573621.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P0A9G0; -.
DR   SMR; P0A9G0; -.
DR   STRING; 155864.EDL933_5150; -.
DR   EnsemblBacteria; AAG59024; AAG59024; Z5349.
DR   EnsemblBacteria; BAB38181; BAB38181; ECs_4758.
DR   GeneID; 66672264; -.
DR   GeneID; 915146; -.
DR   KEGG; ece:Z5349; -.
DR   KEGG; ecs:ECs_4758; -.
DR   PATRIC; fig|386585.9.peg.4967; -.
DR   eggNOG; COG0583; Bacteria.
DR   HOGENOM; CLU_039613_6_0_6; -.
DR   OMA; YPVPDDM; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW.
DR   CDD; cd08441; PBP2_MetR; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR005119; LysR_subst-bd.
DR   InterPro; IPR037406; MetR_PBP2.
DR   InterPro; IPR000847; Tscrpt_reg_HTH_LysR.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00126; HTH_1; 1.
DR   Pfam; PF03466; LysR_substrate; 1.
DR   PRINTS; PR00039; HTHLYSR.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS50931; HTH_LYSR; 1.
PE   3: Inferred from homology;
KW   Activator; Amino-acid biosynthesis; Cytoplasm; DNA-binding;
KW   Methionine biosynthesis; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..317
FT                   /note="HTH-type transcriptional regulator MetR"
FT                   /id="PRO_0000105674"
FT   DOMAIN          1..59
FT                   /note="HTH lysR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT   DNA_BIND        19..38
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
SQ   SEQUENCE   317 AA;  35629 MW;  513F5C710803800D CRC64;
     MIEVKHLKTL QALRNCGSLA AAAATLHQTQ SALSHQFSDL EQRLGFRLFV RKSQPLRFTP
     QGEILLQLAN QVLPQISQAL QACNEPQQTR LRIAIECHSC IQWLTPALEN FHKNWPQVEM
     DFKSGVTFDP QPALQQGELD LVMTSDILPR SGLHYSPMFD YEVRLVLAPD HPLAAKTRIT
     PEDLASETLL IYPVQRSRLD VWRHFLQPAG VSPSLKSVDN TLLLIQMVAA RMGIAALPHW
     VVESFERQGL VVTKTLGEGL WSRLYAAVRD GEQRQPVTEA FIRSARNHAC DHLPFVKSAE
     RPTYDAPTVR PGSPARL