METR_ECOLI
ID METR_ECOLI Reviewed; 317 AA.
AC P0A9F9; P19797; Q2M8D3;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2005, sequence version 1.
DT 25-MAY-2022, entry version 121.
DE RecName: Full=HTH-type transcriptional regulator MetR;
GN Name=metR; OrderedLocusNames=b3828, JW3804;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=2205852; DOI=10.1073/pnas.87.18.7076;
RA Maxon M.E., Wigboldus J., Brot N., Weissbach H.;
RT "Structure-function studies on Escherichia coli MetR protein, a putative
RT prokaryotic leucine zipper protein.";
RL Proc. Natl. Acad. Sci. U.S.A. 87:7076-7079(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=1379743; DOI=10.1126/science.1379743;
RA Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.;
RT "Analysis of the Escherichia coli genome: DNA sequence of the region from
RT 84.5 to 86.5 minutes.";
RL Science 257:771-778(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-19.
RX PubMed=2643109; DOI=10.1073/pnas.86.1.85;
RA Maxon M.E., Redfield B., Cai X.-Y., Shoeman R., Fujita K., Fisher W.,
RA Stauffer G., Weissbach H., Brot N.;
RT "Regulation of methionine synthesis in Escherichia coli: effect of the MetR
RT protein on the expression of the metE and metR genes.";
RL Proc. Natl. Acad. Sci. U.S.A. 86:85-89(1989).
CC -!- FUNCTION: Control of the last step in methionine biosynthesis; MetR is
CC a positive activator of the metA, metE and metH genes. MetR is also a
CC negative regulator of its own expression. Binds homocysteine as an
CC inducer.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the LysR transcriptional regulatory family.
CC {ECO:0000305}.
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DR EMBL; M37630; AAA62781.1; -; Genomic_DNA.
DR EMBL; M87049; AAA67624.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76831.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77473.1; -; Genomic_DNA.
DR EMBL; J04155; AAA24159.2; ALT_SEQ; Genomic_DNA.
DR PIR; A36066; A36066.
DR RefSeq; NP_418272.1; NC_000913.3.
DR RefSeq; WP_000573621.1; NZ_SSZK01000046.1.
DR PDB; 5FO5; X-ray; 2.16 A; A=1-92.
DR PDBsum; 5FO5; -.
DR AlphaFoldDB; P0A9F9; -.
DR SMR; P0A9F9; -.
DR BioGRID; 4259476; 2.
DR BioGRID; 852608; 2.
DR DIP; DIP-10198N; -.
DR IntAct; P0A9F9; 8.
DR STRING; 511145.b3828; -.
DR PaxDb; P0A9F9; -.
DR PRIDE; P0A9F9; -.
DR EnsemblBacteria; AAC76831; AAC76831; b3828.
DR EnsemblBacteria; BAE77473; BAE77473; BAE77473.
DR GeneID; 66672264; -.
DR GeneID; 948310; -.
DR KEGG; ecj:JW3804; -.
DR KEGG; eco:b3828; -.
DR PATRIC; fig|1411691.4.peg.2880; -.
DR EchoBASE; EB0586; -.
DR eggNOG; COG0583; Bacteria.
DR HOGENOM; CLU_039613_6_0_6; -.
DR InParanoid; P0A9F9; -.
DR OMA; YPVPDDM; -.
DR PhylomeDB; P0A9F9; -.
DR BioCyc; EcoCyc:PD03938; -.
DR PRO; PR:P0A9F9; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016597; F:amino acid binding; IDA:EcoCyc.
DR GO; GO:0003677; F:DNA binding; IDA:EcoCyc.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:EcoCyc.
DR CDD; cd08441; PBP2_MetR; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR005119; LysR_subst-bd.
DR InterPro; IPR037406; MetR_PBP2.
DR InterPro; IPR000847; Tscrpt_reg_HTH_LysR.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00126; HTH_1; 1.
DR Pfam; PF03466; LysR_substrate; 1.
DR PRINTS; PR00039; HTHLYSR.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS50931; HTH_LYSR; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Amino-acid biosynthesis; Cytoplasm; DNA-binding;
KW Methionine biosynthesis; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..317
FT /note="HTH-type transcriptional regulator MetR"
FT /id="PRO_0000105673"
FT DOMAIN 1..59
FT /note="HTH lysR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT DNA_BIND 19..38
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT CONFLICT 19
FT /note="L -> T (in Ref. 5)"
FT /evidence="ECO:0000305"
FT HELIX 4..16
FT /evidence="ECO:0007829|PDB:5FO5"
FT HELIX 19..25
FT /evidence="ECO:0007829|PDB:5FO5"
FT HELIX 30..44
FT /evidence="ECO:0007829|PDB:5FO5"
FT HELIX 60..83
FT /evidence="ECO:0007829|PDB:5FO5"
SQ SEQUENCE 317 AA; 35629 MW; 513F5C710803800D CRC64;
MIEVKHLKTL QALRNCGSLA AAAATLHQTQ SALSHQFSDL EQRLGFRLFV RKSQPLRFTP
QGEILLQLAN QVLPQISQAL QACNEPQQTR LRIAIECHSC IQWLTPALEN FHKNWPQVEM
DFKSGVTFDP QPALQQGELD LVMTSDILPR SGLHYSPMFD YEVRLVLAPD HPLAAKTRIT
PEDLASETLL IYPVQRSRLD VWRHFLQPAG VSPSLKSVDN TLLLIQMVAA RMGIAALPHW
VVESFERQGL VVTKTLGEGL WSRLYAAVRD GEQRQPVTEA FIRSARNHAC DHLPFVKSAE
RPTYDAPTVR PGSPARL
