ID   METZ_MYCTO              Reviewed;         406 AA.
AC   P9WGB4; L0T3J4; P95199; Q7D9W7;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 38.
DE   RecName: Full=O-succinylhomoserine sulfhydrylase {ECO:0000255|HAMAP-Rule:MF_02056};
DE            Short=OSH sulfhydrylase {ECO:0000255|HAMAP-Rule:MF_02056};
DE            Short=OSHS sulfhydrylase {ECO:0000255|HAMAP-Rule:MF_02056};
DE            EC=2.5.1.- {ECO:0000255|HAMAP-Rule:MF_02056};
GN   Name=metZ {ECO:0000255|HAMAP-Rule:MF_02056}; OrderedLocusNames=MT0402;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Catalyzes the formation of L-homocysteine from O-succinyl-L-
CC       homoserine (OSHS) and hydrogen sulfide. {ECO:0000255|HAMAP-
CC       Rule:MF_02056}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=hydrogen sulfide + O-succinyl-L-homoserine = L-homocysteine +
CC         succinate; Xref=Rhea:RHEA:27826, ChEBI:CHEBI:29919,
CC         ChEBI:CHEBI:30031, ChEBI:CHEBI:57661, ChEBI:CHEBI:58199;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_02056};
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_02056};
CC   -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de novo
CC       pathway; L-homocysteine from O-succinyl-L-homoserine: step 1/1.
CC       {ECO:0000255|HAMAP-Rule:MF_02056}.
CC   -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_02056}.
CC   -!- SIMILARITY: Belongs to the trans-sulfuration enzymes family. MetZ
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_02056}.
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DR   EMBL; AE000516; AAK44624.1; -; Genomic_DNA.
DR   PIR; F70632; F70632.
DR   RefSeq; WP_003401927.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WGB4; -.
DR   SMR; P9WGB4; -.
DR   EnsemblBacteria; AAK44624; AAK44624; MT0402.
DR   KEGG; mtc:MT0402; -.
DR   PATRIC; fig|83331.31.peg.430; -.
DR   HOGENOM; CLU_018986_2_0_11; -.
DR   UniPathway; UPA00051; UER00449.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0030170; F:pyridoxal phosphate binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016765; F:transferase activity, transferring alkyl or aryl (other than methyl) groups; IEA:UniProtKB-UniRule.
DR   GO; GO:0071266; P:'de novo' L-methionine biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0071268; P:homocysteine biosynthetic process; IEA:InterPro.
DR   GO; GO:0019346; P:transsulfuration; IEA:InterPro.
DR   CDD; cd00614; CGS_like; 1.
DR   Gene3D; 3.40.640.10; -; 1.
DR   Gene3D; 3.90.1150.10; -; 1.
DR   HAMAP; MF_02056; MetZ; 1.
DR   InterPro; IPR000277; Cys/Met-Metab_PyrdxlP-dep_enz.
DR   InterPro; IPR006234; O-succ-hSer_sulfhydrylase.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR   PANTHER; PTHR11808; PTHR11808; 1.
DR   Pfam; PF01053; Cys_Met_Meta_PP; 1.
DR   PIRSF; PIRSF001434; CGS; 1.
DR   SUPFAM; SSF53383; SSF53383; 1.
DR   TIGRFAMs; TIGR01325; O_suc_HS_sulf; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Methionine biosynthesis; Pyridoxal phosphate;
KW   Transferase.
FT   CHAIN           1..406
FT                   /note="O-succinylhomoserine sulfhydrylase"
FT                   /id="PRO_0000428391"
FT   MOD_RES         219
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02056"
SQ   SEQUENCE   406 AA;  43345 MW;  E5C405B25297D04B CRC64;
     MTDESSVRTP KALPDGVSQA TVGVRGGMLR SGFEETAEAM YLTSGYVYGS AAVAEKSFAG
     ELDHYVYSRY GNPTVSVFEE RLRLIEGAPA AFATASGMAA VFTSLGALLG AGDRLVAARS
     LFGSCFVVCS EILPRWGVQT VFVDGDDLSQ WERALSVPTQ AVFFETPSNP MQSLVDIAAV
     TELAHAAGAK VVLDNVFATP LLQQGFPLGV DVVVYSGTKH IDGQGRVLGG AILGDREYID
     GPVQKLMRHT GPAMSAFNAW VLLKGLETLA IRVQHSNASA QRIAEFLNGH PSVRWVRYPY
     LPSHPQYDLA KRQMSGGGTV VTFALDCPED VAKQRAFEVL DKMRLIDISN NLGDAKSLVT
     HPATTTHRAM GPEGRAAIGL GDGVVRISVG LEDTDDLIAD IDRALS