MEX67_CANAL
ID MEX67_CANAL Reviewed; 617 AA.
AC P84149; A0A1D8PSN9; Q5A6R6;
DT 10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 2.
DT 25-MAY-2022, entry version 106.
DE RecName: Full=mRNA export factor MEX67;
GN Name=MEX67 {ECO:0000250|UniProtKB:Q99257};
GN OrderedLocusNames=CAALFM_CR04050CA; ORFNames=CaO19.488, CaO19.8118;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 295-512 IN COMPLEX WITH MTR2.
RX PubMed=14504280; DOI=10.1074/jbc.m308275200;
RA Senay C., Ferrari P., Rocher C., Rieger K.J., Winter J., Platel D.,
RA Bourne Y.;
RT "The Mtr2-Mex67 NTF2-like domain complex. Structural insights into a dual
RT role of Mtr2 for yeast nuclear export.";
RL J. Biol. Chem. 278:48395-48403(2003).
CC -!- FUNCTION: Involved in the export of mRNA from the nucleus to the
CC cytoplasm. {ECO:0000250|UniProtKB:Q99257, ECO:0000255|PROSITE-
CC ProRule:PRU00611}.
CC -!- SUBUNIT: Interacts with nucleoporin complex protein MTR2.
CC {ECO:0000269|PubMed:14504280}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00611}.
CC Cytoplasm {ECO:0000255|PROSITE-ProRule:PRU00611}. Note=Localizes at
CC both the nuclear and cytoplasmic site of the pores. Shuttles between
CC the nucleus and the cytoplasm. {ECO:0000255|PROSITE-ProRule:PRU00611}.
CC -!- DOMAIN: The NTF2 domain heterodimerizes with MTR2. The formation of
CC this heterodimer is essential for mRNA export and binds to all of the
CC nucleoporin-FG-repeats. {ECO:0000250|UniProtKB:Q99257,
CC ECO:0000269|PubMed:14504280}.
CC -!- DOMAIN: The RNA-binding domain is conserved in most NXF proteins but
CC may be absent in yeasts.
CC -!- SIMILARITY: Belongs to the NXF family. {ECO:0000255}.
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DR EMBL; CP017630; AOW31143.1; -; Genomic_DNA.
DR RefSeq; XP_717351.1; XM_712258.2.
DR PDB; 1Q40; X-ray; 1.95 A; B/D=295-512.
DR PDBsum; 1Q40; -.
DR AlphaFoldDB; P84149; -.
DR SMR; P84149; -.
DR BioGRID; 1224015; 1.
DR STRING; 237561.P84149; -.
DR GeneID; 3641020; -.
DR KEGG; cal:CAALFM_CR04050CA; -.
DR CGD; CAL0000181331; MEX67.
DR VEuPathDB; FungiDB:CR_04050C_A; -.
DR eggNOG; KOG3763; Eukaryota.
DR HOGENOM; CLU_024991_1_1_1; -.
DR InParanoid; P84149; -.
DR OMA; YGGHEAW; -.
DR OrthoDB; 1051093at2759; -.
DR EvolutionaryTrace; P84149; -.
DR PRO; PR:P84149; -.
DR Proteomes; UP000000559; Chromosome R.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0016973; P:poly(A)+ mRNA export from nucleus; IBA:GO_Central.
DR CDD; cd14342; UBA_TAP-C; 1.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR040736; Mex67_RRM.
DR InterPro; IPR032710; NTF2-like_dom_sf.
DR InterPro; IPR018222; Nuclear_transport_factor_2_euk.
DR InterPro; IPR030217; NXF_fam.
DR InterPro; IPR005637; TAP_C_dom.
DR InterPro; IPR009060; UBA-like_sf.
DR PANTHER; PTHR10662; PTHR10662; 1.
DR Pfam; PF18444; RRM_9; 1.
DR Pfam; PF03943; TAP_C; 1.
DR SMART; SM00804; TAP_C; 1.
DR SUPFAM; SSF46934; SSF46934; 1.
DR SUPFAM; SSF54427; SSF54427; 1.
DR PROSITE; PS51450; LRR; 3.
DR PROSITE; PS50177; NTF2_DOMAIN; 1.
DR PROSITE; PS51281; TAP_C; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Leucine-rich repeat; mRNA transport; Nucleus;
KW Reference proteome; Repeat; Transport.
FT CHAIN 1..617
FT /note="mRNA export factor MEX67"
FT /id="PRO_0000220540"
FT REPEAT 185..206
FT /note="LRR 1"
FT REPEAT 211..232
FT /note="LRR 2"
FT REPEAT 237..258
FT /note="LRR 3"
FT DOMAIN 309..499
FT /note="NTF2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00137"
FT DOMAIN 565..617
FT /note="TAP-C"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00611"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 442..469
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 513..554
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 513..533
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 307..322
FT /evidence="ECO:0007829|PDB:1Q40"
FT HELIX 325..331
FT /evidence="ECO:0007829|PDB:1Q40"
FT STRAND 337..342
FT /evidence="ECO:0007829|PDB:1Q40"
FT TURN 364..369
FT /evidence="ECO:0007829|PDB:1Q40"
FT TURN 373..375
FT /evidence="ECO:0007829|PDB:1Q40"
FT HELIX 379..384
FT /evidence="ECO:0007829|PDB:1Q40"
FT HELIX 389..398
FT /evidence="ECO:0007829|PDB:1Q40"
FT STRAND 402..404
FT /evidence="ECO:0007829|PDB:1Q40"
FT TURN 407..409
FT /evidence="ECO:0007829|PDB:1Q40"
FT HELIX 411..413
FT /evidence="ECO:0007829|PDB:1Q40"
FT STRAND 415..420
FT /evidence="ECO:0007829|PDB:1Q40"
FT STRAND 427..439
FT /evidence="ECO:0007829|PDB:1Q40"
FT STRAND 460..464
FT /evidence="ECO:0007829|PDB:1Q40"
FT STRAND 474..483
FT /evidence="ECO:0007829|PDB:1Q40"
FT STRAND 491..501
FT /evidence="ECO:0007829|PDB:1Q40"
SQ SEQUENCE 617 AA; 68257 MW; F014C89D5F56AC2D CRC64;
MSYRGRGGGY NNNRGQFSSG PHQHQQNVDS FVAANQYPIE IMGWNGASSG ECINFISRKC
KVIVSNYSVD SNSGVLKGYV KNESQANTLL NWSGVKFAGQ SLRFSKGVSN ISNQMGGGAS
TGSQSTIETI SQFLKARYQP EIKMLNLSNV KQDPTLTAQG FFGSLSVSSK FFPALMKVAS
DLKLDVDSID LSNNELQDLQ TLTSMAQTFP KLQNLSLQNN NFTKIKVFET WRHKLNFLRE
LILFNNPIVQ TNDPAEIQTI KLELMKSFPR LVVLSGEILR NEQVLIANLS FPFESPETMF
FQDEDSRNLA TNFIANYLKL WDANRSELMI LYQNESQFSM QVDSSHPHLI ESGNSGYSGS
TDFGYYLNNS RNLTRVSSIK ARMAKLSIGQ EQIYKSFQQL PKTRHDIIAT PELFSMEVYK
FPTLNGIMIT LHGSFDEVAQ PEVDGSASSA PSGPRGGSRY HSGPKHKRIP LSKKSFDRTF
VVIPGPNGSM IVASDTLLIR PYTSDFPWKV QKLPSNPTAA TPGVSATSTP SPLPPTTITT
PQLAPPGTGP TTADLPADIK ARLNQIQQEL LVKILLETKL NINYGIMLCE QSNWDYQQAS
VNFKNSAASL PSDAFVQ
