ID   MF227_ASPFN             Reviewed;         423 AA.
AC   B8MYS8;
DT   11-MAY-2016, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 57.
DE   RecName: Full=Probable efflux pump mfs2 {ECO:0000305};
DE   AltName: Full=Asparasone A synthesis protein mfs2 {ECO:0000305};
GN   Name=mfs2 {ECO:0000303|PubMed:24412484}; ORFNames=AFLA_082170;
OS   Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357
OS   / JCM 12722 / SRRC 167).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=332952;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC
RC   167;
RX   PubMed=25883274; DOI=10.1128/genomea.00168-15;
RA   Nierman W.C., Yu J., Fedorova-Abrams N.D., Losada L., Cleveland T.E.,
RA   Bhatnagar D., Bennett J.W., Dean R., Payne G.A.;
RT   "Genome sequence of Aspergillus flavus NRRL 3357, a strain that causes
RT   aflatoxin contamination of food and feed.";
RL   Genome Announc. 3:E0016815-E0016815(2015).
RN   [2]
RP   INDUCTION.
RX   PubMed=24412484; DOI=10.1016/j.fgb.2014.01.001;
RA   Cary J.W., Harris-Coward P.Y., Ehrlich K.C., Di Mavungu J.D.,
RA   Malysheva S.V., De Saeger S., Dowd P.F., Shantappa S., Martens S.L.,
RA   Calvo A.M.;
RT   "Functional characterization of a veA-dependent polyketide synthase gene in
RT   Aspergillus flavus necessary for the synthesis of asparasone, a sclerotium-
RT   specific pigment.";
RL   Fungal Genet. Biol. 64:25-35(2014).
CC   -!- FUNCTION: Probable efflux pump; part of the gene cluster 27 that
CC       mediates the biosynthesis of asparasone A, a sclerotium-specific
CC       anthraquinone pigment important for sclerotial survival
CC       (PubMed:24412484). {ECO:0000269|PubMed:24412484}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- INDUCTION: Expression is induced by the developmental and secondary
CC       metabolism regulator veA (PubMed:24412484).
CC       {ECO:0000269|PubMed:24412484}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       {ECO:0000305}.
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DR   EMBL; EQ963472; EED57520.1; -; Genomic_DNA.
DR   RefSeq; XP_002373132.1; XM_002373091.1.
DR   AlphaFoldDB; B8MYS8; -.
DR   SMR; B8MYS8; -.
DR   EnsemblFungi; EED57520; EED57520; AFLA_082170.
DR   VEuPathDB; FungiDB:AFLA_082170; -.
DR   eggNOG; KOG0255; Eukaryota.
DR   HOGENOM; CLU_008455_1_3_1; -.
DR   OMA; NIWFLVW; -.
DR   Proteomes; UP000001875; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR005829; Sugar_transporter_CS.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
DR   PROSITE; PS00216; SUGAR_TRANSPORT_1; 1.
PE   2: Evidence at transcript level;
KW   Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..423
FT                   /note="Probable efflux pump mfs2"
FT                   /id="PRO_0000436131"
FT   TRANSMEM        21..41
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        49..69
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        79..99
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        111..131
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        138..158
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        220..240
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        256..278
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        295..315
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        319..339
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        360..380
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        392..411
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   423 AA;  46210 MW;  0C3BD06BFCFE04B4 CRC64;
     MMAPSIDTMA EELDTSITES TMALSVYLLA TAFGPLIIGP VSEIYGRKSI FHITNIWFLV
     WNLVCGFAHS KGLLMAARLL AGFGASAVYS LGYGVLGDVW SAEQRGRSLS LYLLIPLTGS
     AVGPIVSGFI VKYSTWRWMF WSTAILQLTL DLSSLLFHES YAPLLLRRRA EELRSNTGGS
     RYHAAIEMRE AGLSPPRKLS RSLSRPLRLL AFHPIIQMQA ILEGIDYGLL YFALSSFSAL
     HVAAYGESVE ISGLHYIVIC IGTVSGSQLC GPLMDYAYQR LSSNTGETQV PELRIPLLLP
     GALITPIGFL LYGWAAQYHL IWVVVDVGAA LLSLGMQIFD TTLHAYVMDS YPEHVSSASA
     ATQVLRSLLA FAFPLFSNSL YDSLGYGLGN SLLAFLSIGI ALPATGILWR WGATLRGRQQ
     SSY