MFA2_YEAST
ID MFA2_YEAST Reviewed; 38 AA.
AC P34166; D6W138;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Mating hormone A-factor 2;
DE Flags: Precursor;
GN Name=MFA2; OrderedLocusNames=YNL145W; ORFNames=N1204, N1787;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RA Brake A.J., Brenner C., Najarian R., Laybourn P., Merryweather J.;
RT "Structure of genes encoding precursors of the yeast peptide mating
RT pheromone a-factor.";
RL (In) Gething M.-J. (eds.);
RL Protein transport and secretion, pp.103-108, Cold Spring Harbor Laboratory
RL Press, New York (1985).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RX PubMed=3285180; DOI=10.1128/mcb.8.3.1309-1318.1988;
RA Michaelis S., Herskowitz I.;
RT "The a-factor pheromone of Saccharomyces cerevisiae is essential for
RT mating.";
RL Mol. Cell. Biol. 8:1309-1318(1988).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1358757; DOI=10.1101/gad.6.11.2088;
RA Lowell J.E., Rudner D.Z., Sachs A.B.;
RT "3'-UTR-dependent deadenylation by the yeast poly(A) nuclease.";
RL Genes Dev. 6:2088-2099(1992).
RN [4]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=8686380;
RX DOI=10.1002/(sici)1097-0061(199602)12:2<169::aid-yea894>3.0.co;2-b;
RA Nasr F., Becam A.-M., Herbert C.J.;
RT "The sequence of 36.8 kb from the left arm of chromosome XIV reveals 24
RT complete open reading frames: 18 correspond to new genes, one of which
RT encodes a protein similar to the human myotonic dystrophy kinase.";
RL Yeast 12:169-175(1996).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8619318; DOI=10.1002/yea.320111210;
RA Mallet L., Bussereau F., Jacquet M.;
RT "A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2,
RT CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine
RT deaminase gene and 14 new open reading frames.";
RL Yeast 11:1195-1209(1995).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169873;
RA Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT evolutionary implications.";
RL Nature 387:93-98(1997).
RN [7]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [8]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [9]
RP PROTEIN SEQUENCE OF 24-35.
RX PubMed=3542988; DOI=10.1016/s0021-9258(19)75817-7;
RA Betz R., Crabb J.W., Meyer H.E., Wittig R., Duntze W.;
RT "Amino acid sequences of a-factor mating peptides from Saccharomyces
RT cerevisiae.";
RL J. Biol. Chem. 262:546-548(1987).
RN [10]
RP ISOPRENYLATION AT CYS-35, AND METHYLATION AT CYS-35.
RX PubMed=3056940; DOI=10.1016/s0021-9258(19)81351-0;
RA Anderegg R.J., Betz R., Carr S.A., Crabb J.W., Duntze W.;
RT "Structure of Saccharomyces cerevisiae mating hormone a-factor.
RT Identification of S-farnesyl cysteine as a structural component.";
RL J. Biol. Chem. 263:18236-18240(1988).
CC -!- FUNCTION: The active factor is excreted into the culture medium by
CC haploid cells of the A mating type and acts on cells of the opposite
CC mating type (type alpha). It mediates the conjugation process between
CC the two types by inhibiting the initiation of DNA synthesis in type
CC alpha cells and synchronizing them with type A.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}; Cytoplasmic side {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U20823; AAA68602.1; -; Genomic_DNA.
DR EMBL; U26204; AAA67688.1; -; Genomic_DNA.
DR EMBL; Z46843; CAA86881.1; -; Genomic_DNA.
DR EMBL; S48033; AAD13854.1; -; mRNA.
DR EMBL; Z71421; CAA96028.1; -; Genomic_DNA.
DR EMBL; AY693189; AAT93208.1; -; Genomic_DNA.
DR EMBL; BK006947; DAA10404.1; -; Genomic_DNA.
DR PIR; S55139; S55139.
DR RefSeq; NP_014254.1; NM_001182983.1.
DR AlphaFoldDB; P34166; -.
DR BioGRID; 35683; 92.
DR DIP; DIP-2140N; -.
DR IntAct; P34166; 2.
DR STRING; 4932.YNL145W; -.
DR PaxDb; P34166; -.
DR TopDownProteomics; P34166; -.
DR EnsemblFungi; YNL145W_mRNA; YNL145W; YNL145W.
DR GeneID; 855577; -.
DR KEGG; sce:YNL145W; -.
DR SGD; S000005089; MFA2.
DR VEuPathDB; FungiDB:YNL145W; -.
DR eggNOG; ENOG502SCDB; Eukaryota.
DR GeneTree; ENSGT00940000180742; -.
DR HOGENOM; CLU_220658_0_0_1; -.
DR InParanoid; P34166; -.
DR BioCyc; YEAST:G3O-33163-MON; -.
DR PRO; PR:P34166; -.
DR Proteomes; UP000002311; Chromosome XIV.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0005576; C:extracellular region; IDA:SGD.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000772; F:mating pheromone activity; IMP:SGD.
DR GO; GO:0006113; P:fermentation; IDA:SGD.
DR GO; GO:0000750; P:pheromone-dependent signal transduction involved in conjugation with cellular fusion; IMP:SGD.
DR InterPro; IPR035296; Mfa1/2.
DR Pfam; PF17317; MFA1_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Direct protein sequencing; Lipoprotein; Membrane;
KW Methylation; Pheromone; Prenylation; Reference proteome.
FT PROPEP 1..23
FT /evidence="ECO:0000269|PubMed:3542988"
FT /id="PRO_0000021693"
FT PEPTIDE 24..35
FT /note="Mating hormone A-factor 2"
FT /id="PRO_0000021694"
FT PROPEP 36..38
FT /note="Removed in mature form"
FT /id="PRO_0000021695"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 35
FT /note="Cysteine methyl ester"
FT /evidence="ECO:0000269|PubMed:3056940"
FT LIPID 35
FT /note="S-farnesyl cysteine"
FT /evidence="ECO:0000269|PubMed:3056940"
SQ SEQUENCE 38 AA; 4229 MW; C71466AEA2F133C4 CRC64;
MQPITTASTQ ATQKDKSSEK KDNYIIKGLF WDPACVIA
