MFA3L_MOUSE
ID MFA3L_MOUSE Reviewed; 409 AA.
AC Q9D3X9; Q80TV6; Q8BJA9;
DT 05-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Microfibrillar-associated protein 3-like;
DE Flags: Precursor;
GN Name=Mfap3l; Synonyms=Kiaa0626;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 60-409 (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=12693553; DOI=10.1093/dnares/10.1.35;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S.,
RA Nakajima D., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: II.
RT The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:35-48(2003).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-298; SER-303 AND SER-306, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Kidney, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May participate in the nuclear signaling of EGFR and
CC MAPK1/ERK2. {ECO:0000250|UniProtKB:O75121}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:O75121};
CC Single-pass type I membrane protein {ECO:0000250|UniProtKB:O75121}.
CC Nucleus {ECO:0000250|UniProtKB:O75121}. Cytoplasm
CC {ECO:0000250|UniProtKB:O75121}. Note=Mainly localized in the nucleus.
CC {ECO:0000250|UniProtKB:O75121}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9D3X9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9D3X9-2; Sequence=VSP_014096;
CC -!- CAUTION: A protein kinase activity has been reported however PROSITE,
CC Pfam do not detect a protein kinase domain. Its enzyme activity is
CC therefore unsure. {ECO:0000250|UniProtKB:O75121}.
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DR EMBL; AK016959; BAB30523.1; -; mRNA.
DR EMBL; AK089654; BAC40939.1; -; mRNA.
DR EMBL; BC095950; AAH95950.1; -; mRNA.
DR EMBL; AK122332; BAC65614.1; -; mRNA.
DR CCDS; CCDS22321.1; -. [Q9D3X9-1]
DR CCDS; CCDS80877.1; -. [Q9D3X9-2]
DR RefSeq; NP_001171352.1; NM_001177881.1. [Q9D3X9-1]
DR RefSeq; NP_001171353.1; NM_001177882.1. [Q9D3X9-1]
DR RefSeq; NP_001280698.1; NM_001293769.1. [Q9D3X9-2]
DR RefSeq; NP_082032.1; NM_027756.5. [Q9D3X9-1]
DR AlphaFoldDB; Q9D3X9; -.
DR IntAct; Q9D3X9; 1.
DR STRING; 10090.ENSMUSP00000125139; -.
DR GlyGen; Q9D3X9; 5 sites.
DR iPTMnet; Q9D3X9; -.
DR PhosphoSitePlus; Q9D3X9; -.
DR SwissPalm; Q9D3X9; -.
DR MaxQB; Q9D3X9; -.
DR PaxDb; Q9D3X9; -.
DR PeptideAtlas; Q9D3X9; -.
DR PRIDE; Q9D3X9; -.
DR ProteomicsDB; 295558; -. [Q9D3X9-1]
DR ProteomicsDB; 295559; -. [Q9D3X9-2]
DR Antibodypedia; 17138; 105 antibodies from 20 providers.
DR DNASU; 71306; -.
DR Ensembl; ENSMUST00000034066; ENSMUSP00000034066; ENSMUSG00000031647. [Q9D3X9-1]
DR Ensembl; ENSMUST00000160719; ENSMUSP00000125139; ENSMUSG00000031647. [Q9D3X9-1]
DR Ensembl; ENSMUST00000161421; ENSMUSP00000124136; ENSMUSG00000031647. [Q9D3X9-2]
DR Ensembl; ENSMUST00000161702; ENSMUSP00000124330; ENSMUSG00000031647. [Q9D3X9-1]
DR GeneID; 71306; -.
DR KEGG; mmu:71306; -.
DR UCSC; uc009ltf.3; mouse. [Q9D3X9-1]
DR CTD; 9848; -.
DR MGI; MGI:1918556; Mfap3l.
DR VEuPathDB; HostDB:ENSMUSG00000031647; -.
DR eggNOG; ENOG502QW9J; Eukaryota.
DR GeneTree; ENSGT00390000011576; -.
DR HOGENOM; CLU_056017_2_0_1; -.
DR InParanoid; Q9D3X9; -.
DR OMA; GGKWWLL; -.
DR OrthoDB; 1154642at2759; -.
DR PhylomeDB; Q9D3X9; -.
DR TreeFam; TF333205; -.
DR BioGRID-ORCS; 71306; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Mfap3l; mouse.
DR PRO; PR:Q9D3X9; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q9D3X9; protein.
DR Bgee; ENSMUSG00000031647; Expressed in lacrimal gland and 215 other tissues.
DR Genevisible; Q9D3X9; MM.
DR GO; GO:0030054; C:cell junction; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR013098; Ig_I-set.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR003598; Ig_sub2.
DR InterPro; IPR039696; MFAP3-like.
DR PANTHER; PTHR14340; PTHR14340; 1.
DR Pfam; PF07679; I-set; 1.
DR SMART; SM00409; IG; 1.
DR SMART; SM00408; IGc2; 1.
DR SUPFAM; SSF48726; SSF48726; 1.
DR PROSITE; PS50835; IG_LIKE; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Cytoplasm; Disulfide bond;
KW Glycoprotein; Immunoglobulin domain; Membrane; Nucleus; Phosphoprotein;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT CHAIN 29..409
FT /note="Microfibrillar-associated protein 3-like"
FT /id="PRO_0000014870"
FT TOPO_DOM 29..148
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 149..169
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 170..409
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 47..141
FT /note="Ig-like C2-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT REGION 319..395
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 322..343
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 358..373
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 287
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:O75121"
FT MOD_RES 298
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 303
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 306
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 307
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6AYP2"
FT CARBOHYD 33
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 37
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 67
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 111
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 135
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 68..125
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT VAR_SEQ 1..103
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_014096"
FT CONFLICT 283
FT /note="R -> G (in Ref. 1; BAC40939)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 409 AA; 45342 MW; BC0B3FA7568AA4C8 CRC64;
MGLQKSHLTV CLPPSVPFLI LVSTLATAKS VTNSTLNGTD VVLGSVPVII ARTDHIIVKE
GSSALINCSA YGFPDLEFKW YNSVGKLLKE MDDEKEKGGG KWQMLDGGLL NITKVSFSDR
GKYTCVASNI YGTINNTVTL RVIFTSGDMG VYYMVVCLVA FTIVMILNIT RLCMMSSHLK
KTEKAINEFF RTEGAEKLQK AFEIAKRIPI ITSAKTLELA KVTQFKTMEF ARYIEELARS
VPLPPLIMNC RTIMEEIMEV VGLEEQGQNF VRHTPEGQEA PDRDEVYTIP NSLKRSESPT
ADSDASSLHE QPQQIAIKVS VHPQSKRDHV DDQEGGHFEV KDEEETEPSE EHSPETAEPS
TDITTTELTS EETSPVEAPE RGLPPAHLET TEPAVTCDRN TCIIYESHV
