MFA5_PORG3
ID MFA5_PORG3 Reviewed; 1228 AA.
AC B2RHG5;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-2008, sequence version 1.
DT 03-AUG-2022, entry version 56.
DE RecName: Full=Minor fimbrium subunit Mfa5;
DE Flags: Precursor;
GN OrderedLocusNames=PGN_0291 {ECO:0000312|EMBL:BAG32810.1};
OS Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / CIP 103683 / JCM
OS 12257 / NCTC 11834 / 2561).
OC Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Porphyromonadaceae;
OC Porphyromonas.
OX NCBI_TaxID=431947;
RN [1] {ECO:0000312|EMBL:BAG32810.1, ECO:0000312|Proteomes:UP000008842}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33277 / DSM 20709 / CIP 103683 / JCM 12257 / NCTC 11834 / 2561
RC {ECO:0000312|Proteomes:UP000008842};
RX PubMed=18524787; DOI=10.1093/dnares/dsn013;
RA Naito M., Hirakawa H., Yamashita A., Ohara N., Shoji M., Yukitake H.,
RA Nakayama K., Toh H., Yoshimura F., Kuhara S., Hattori M., Hayashi T.,
RA Nakayama K.;
RT "Determination of the genome sequence of Porphyromonas gingivalis strain
RT ATCC 33277 and genomic comparison with strain W83 revealed extensive genome
RT rearrangements in P. gingivalis.";
RL DNA Res. 15:215-225(2008).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, AND SUBUNIT.
RC STRAIN=ATCC 33277 / DSM 20709 / CIP 103683 / JCM 12257 / NCTC 11834 / 2561
RC {ECO:0000303|PubMed:19589838};
RX PubMed=19589838; DOI=10.1099/mic.0.028928-0;
RA Hasegawa Y., Iwami J., Sato K., Park Y., Nishikawa K., Atsumi T.,
RA Moriguchi K., Murakami Y., Lamont R.J., Nakamura H., Ohno N., Yoshimura F.;
RT "Anchoring and length regulation of Porphyromonas gingivalis Mfa1 fimbriae
RT by the downstream gene product Mfa2.";
RL Microbiology 155:3333-3347(2009).
RN [3]
RP SUBCELLULAR LOCATION, AND SUBUNIT.
RC STRAIN=ATCC 33277 / DSM 20709 / CIP 103683 / JCM 12257 / NCTC 11834 / 2561
RC {ECO:0000303|PubMed:24118823};
RX PubMed=24118823; DOI=10.1111/omi.12040;
RA Hasegawa Y., Nagano K., Ikai R., Izumigawa M., Yoshida Y., Kitai N.,
RA Lamont R.J., Murakami Y., Yoshimura F.;
RT "Localization and function of the accessory protein Mfa3 in Porphyromonas
RT gingivalis Mfa1 fimbriae.";
RL Mol. Oral. Microbiol. 28:467-480(2013).
RN [4]
RP FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RC STRAIN=ATCC 33277 / DSM 20709 / CIP 103683 / JCM 12257 / NCTC 11834 / 2561
RC {ECO:0000303|PubMed:26001707};
RX PubMed=26001707; DOI=10.1177/0022034515588275;
RA Nagano K., Hasegawa Y., Yoshida Y., Yoshimura F.;
RT "A major fimbrilin variant of Mfa1 fimbriae in Porphyromonas gingivalis.";
RL J. Dent. Res. 94:1143-1148(2015).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, AND SUBUNIT.
RC STRAIN=ATCC 33277 / DSM 20709 / CIP 103683 / JCM 12257 / NCTC 11834 / 2561
RC {ECO:0000303|PubMed:26437277};
RX PubMed=26437277; DOI=10.1371/journal.pone.0139454;
RA Ikai R., Hasegawa Y., Izumigawa M., Nagano K., Yoshida Y., Kitai N.,
RA Lamont R.J., Yoshimura F., Murakami Y.;
RT "Mfa4, an accessory protein of Mfa1 fimbriae, modulates fimbrial
RT biogenesis, cell auto-aggregation, and biofilm formation in Porphyromonas
RT gingivalis.";
RL PLoS ONE 10:E0139454-E0139454(2015).
CC -!- FUNCTION: Accessory subunit of the minor fimbriae. These filamentous
CC pili are attached to the cell surface; they mediate biofilm formation,
CC adhesion onto host cells and onto other bacteria that are part of the
CC oral microbiome (PubMed:26001707). They play an important role in
CC invasion of periodontal tissues and are recognized as major virulence
CC factors. Fimbrium subunits from different strains have highly divergent
CC sequences, and this correlates with pathogenicity (Probable).
CC {ECO:0000269|PubMed:26001707, ECO:0000305}.
CC -!- SUBUNIT: Minor fimbriae are composed of a structural subunit, most
CC often Mfa1, and the accessory subunits Mfa3, Mfa4 and Mfa5
CC (PubMed:19589838, PubMed:24118823, PubMed:26001707, PubMed:26437277).
CC Fimbrium assembly occurs by linear, head-to-tail oligomerization of
CC fimbrial subunits. This is mediated via insertion of a C-terminal beta-
CC strand from one subunit into a groove in the N-terminal domain of the
CC following subunit (Probable). {ECO:0000269|PubMed:19589838,
CC ECO:0000269|PubMed:24118823, ECO:0000269|PubMed:26001707,
CC ECO:0000269|PubMed:26437277, ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Fimbrium {ECO:0000269|PubMed:19589838,
CC ECO:0000269|PubMed:24118823, ECO:0000269|PubMed:26001707,
CC ECO:0000269|PubMed:26437277}.
CC -!- MISCELLANEOUS: The name (minor fimbrium subunit) does not indicate the
CC abundance of the protein, but is derived from the greater length of the
CC major fimbriae. In strain ATCC 33277 and strain ATCC BAA-1703 / FDC
CC 381, major fimbriae are 300 - 1600 nM in length and about 5 nm in
CC diameter. In contrast, minor fimbriae are only about 80 - 120 nm long.
CC This length difference is observed only in a small number of strains,
CC including strain ATCC 33277 and strain ATCC BAA-1703 / FDC 381, and is
CC due to a loss of function mutation in FimB, a protein that restricts
CC fimbrial length in other strains. {ECO:0000305}.
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DR EMBL; AP009380; BAG32810.1; -; Genomic_DNA.
DR RefSeq; WP_012457399.1; NZ_CP025930.1.
DR PDB; 6TNJ; X-ray; 1.85 A; A=138-435.
DR PDB; 6TO1; X-ray; 1.80 A; A=21-1044.
DR PDBsum; 6TNJ; -.
DR PDBsum; 6TO1; -.
DR AlphaFoldDB; B2RHG5; -.
DR SMR; B2RHG5; -.
DR STRING; 431947.PGN_0291; -.
DR PRIDE; B2RHG5; -.
DR EnsemblBacteria; BAG32810; BAG32810; PGN_0291.
DR GeneID; 29255537; -.
DR KEGG; pgn:PGN_0291; -.
DR eggNOG; ENOG5032FSV; Bacteria.
DR HOGENOM; CLU_007353_0_0_10; -.
DR OMA; MARNIMA; -.
DR BioCyc; PGIN431947:G1G2V-318-MON; -.
DR Proteomes; UP000008842; Chromosome.
DR GO; GO:0009289; C:pilus; IDA:UniProtKB.
DR Gene3D; 3.40.50.410; -; 1.
DR InterPro; IPR002035; VWF_A.
DR InterPro; IPR036465; vWFA_dom_sf.
DR Pfam; PF13519; VWA_2; 1.
DR SMART; SM00327; VWA; 1.
DR SUPFAM; SSF53300; SSF53300; 1.
DR PROSITE; PS50234; VWFA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Fimbrium; Signal; Virulence.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..1228
FT /note="Minor fimbrium subunit Mfa5"
FT /id="PRO_0000436800"
FT DOMAIN 144..267
FT /note="VWFA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00219"
FT STRAND 107..115
FT /evidence="ECO:0007829|PDB:6TO1"
FT STRAND 125..136
FT /evidence="ECO:0007829|PDB:6TO1"
FT STRAND 143..150
FT /evidence="ECO:0007829|PDB:6TO1"
FT HELIX 153..155
FT /evidence="ECO:0007829|PDB:6TO1"
FT HELIX 157..159
FT /evidence="ECO:0007829|PDB:6TO1"
FT HELIX 160..177
FT /evidence="ECO:0007829|PDB:6TO1"
FT HELIX 181..183
FT /evidence="ECO:0007829|PDB:6TO1"
FT STRAND 185..205
FT /evidence="ECO:0007829|PDB:6TO1"
FT HELIX 208..216
FT /evidence="ECO:0007829|PDB:6TO1"
FT HELIX 226..237
FT /evidence="ECO:0007829|PDB:6TO1"
FT STRAND 243..251
FT /evidence="ECO:0007829|PDB:6TO1"
FT HELIX 266..268
FT /evidence="ECO:0007829|PDB:6TO1"
FT HELIX 279..289
FT /evidence="ECO:0007829|PDB:6TO1"
FT HELIX 291..294
FT /evidence="ECO:0007829|PDB:6TO1"
FT STRAND 313..315
FT /evidence="ECO:0007829|PDB:6TO1"
FT HELIX 321..323
FT /evidence="ECO:0007829|PDB:6TO1"
FT STRAND 341..344
FT /evidence="ECO:0007829|PDB:6TO1"
FT STRAND 353..355
FT /evidence="ECO:0007829|PDB:6TO1"
FT HELIX 357..370
FT /evidence="ECO:0007829|PDB:6TO1"
FT STRAND 374..379
FT /evidence="ECO:0007829|PDB:6TO1"
FT HELIX 386..395
FT /evidence="ECO:0007829|PDB:6TO1"
FT STRAND 396..398
FT /evidence="ECO:0007829|PDB:6TO1"
FT HELIX 399..401
FT /evidence="ECO:0007829|PDB:6TO1"
FT STRAND 402..404
FT /evidence="ECO:0007829|PDB:6TO1"
FT HELIX 407..412
FT /evidence="ECO:0007829|PDB:6TO1"
FT TURN 413..416
FT /evidence="ECO:0007829|PDB:6TO1"
FT STRAND 423..434
FT /evidence="ECO:0007829|PDB:6TO1"
FT STRAND 438..440
FT /evidence="ECO:0007829|PDB:6TO1"
FT STRAND 445..448
FT /evidence="ECO:0007829|PDB:6TO1"
FT HELIX 450..452
FT /evidence="ECO:0007829|PDB:6TO1"
FT STRAND 453..463
FT /evidence="ECO:0007829|PDB:6TO1"
FT TURN 464..467
FT /evidence="ECO:0007829|PDB:6TO1"
FT STRAND 468..472
FT /evidence="ECO:0007829|PDB:6TO1"
FT STRAND 476..490
FT /evidence="ECO:0007829|PDB:6TO1"
FT HELIX 492..497
FT /evidence="ECO:0007829|PDB:6TO1"
FT STRAND 515..527
FT /evidence="ECO:0007829|PDB:6TO1"
FT STRAND 534..537
FT /evidence="ECO:0007829|PDB:6TO1"
FT STRAND 546..557
FT /evidence="ECO:0007829|PDB:6TO1"
FT HELIX 574..576
FT /evidence="ECO:0007829|PDB:6TO1"
FT STRAND 579..585
FT /evidence="ECO:0007829|PDB:6TO1"
FT TURN 595..597
FT /evidence="ECO:0007829|PDB:6TO1"
FT STRAND 598..600
FT /evidence="ECO:0007829|PDB:6TO1"
FT STRAND 609..613
FT /evidence="ECO:0007829|PDB:6TO1"
FT STRAND 618..621
FT /evidence="ECO:0007829|PDB:6TO1"
FT STRAND 627..631
FT /evidence="ECO:0007829|PDB:6TO1"
FT STRAND 639..645
FT /evidence="ECO:0007829|PDB:6TO1"
FT STRAND 647..649
FT /evidence="ECO:0007829|PDB:6TO1"
FT STRAND 651..660
FT /evidence="ECO:0007829|PDB:6TO1"
SQ SEQUENCE 1228 AA; 134495 MW; F91EF792512D6DE3 CRC64;
MMKRYTIILA VFLLFCTVFT FQIKARPYER FADVEKPWIQ KHSMDSKLVP ANKGNLIQAE
IVYQSVSEHS DLVISPVNEI RPANRFPSHR KSFFAENLRA SPPVVPVAVD KYAVPVANPM
DPENPNAWDV TLKITTKAVT VPVDVVMVID QSSSMGGQNI ARLKSAIASG QRFVKKMLPK
GMATEGVRIA LVSYDHEPHR LSDFTKDTAF LCQKIRALTP IWGTHTQGGL KMARNIMATS
TAVDKHIILM SDGLATEQYP VKNVTTADFI GETGNANDPI DLVIQGAINF PTNYVSNNPS
TPLTPNYPTH SSKVGRRNLP ESKFDYSNLS ARITFDGVAG ALVYEPRFPH PYYYYFPCNA
AINEAQFAKN SGYTIHTIGY DLGDFALANN SLKLTATDEN HFFTATPANL AAAFDNIAQT
INIGIQRGEV TDFVAPGFIV KNLTQSGDVT HLLNVSNGTV HYDVSTKKLT WTTGTILSSS
EATITYRIYA DLDYIQNNDI PVNTTSAIGP DLGGFDTNTE AKLTYTNSNG ESNQQLIFPR
PTVKLGYGVI KRHYVLVNKD GQPIQANGTV VSSLSEAHVL QSQDFFLPSG GGHIVPKWIK
LDKTTEALQY YSVPPTNTVI TTADGKRYRF VEVPGSTPNP GQIGISWKKP AGNAYFAYKL
LNYWMGGTTD QQSEWDVTSN WTGAQVPLTG EDVEFATTEN FGSPAVADLH VPTTNPKIIG
NLINNSDKDL VVTTNSQLTI NGVVEDNNPN VGTIVVKSSK DNPTGTLLFA NPGYNQNVGG
TVEFYNQGYD CADCGMYRRS WQYFGIPVNE SGFPINDVGG NETVNQWVEP FNGDKWRPAP
YAPDTKLQKF KGYQITNDVQ AQPTGVYSFK GTLCVCDAFL NLTRTSGVNY SGANLIGNSY
TGAIDIKQGI VFPPEVEQTV YLFNTGTRDQ WRKLNGSTVS GFRAGQYLSV PKNTAGQDNL
PDRIPSMHSF LVKMQNGASC TLQILYDKLL KNTTVNNGNG TQITWRSGNS GSANMPSLVM
DVLGNESADR LWIFTDGGLS FGFDNGWDGR KLTEKGLSQL YAMSDIGNDK FQVAGVPELN
NLLIGFDADK DGQYTLEFAL SDHFAKGGVF LEDLSRGVTR RIVDGGSYSF DAKRGDSGAR
FRLSYDEEWV ESAEVSVLVG TVGKRILITN NCEHACQANV YTTDGKLLIR LDVKPGSKSM
TEPLIDGAYV VSLQSPATSS NVRKVVVN
