MFAL2_YEAST
ID MFAL2_YEAST Reviewed; 120 AA.
AC P32435; D6VU56;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Mating factor alpha-2;
DE AltName: Full=Alpha-2 mating pheromone;
DE Contains:
DE RecName: Full=Mating factor alpha;
DE Contains:
DE RecName: Full=Mating factor alpha-like;
DE Flags: Precursor;
GN Name=MF(ALPHA)2; Synonyms=MF-ALPHA-2, MFAL2; OrderedLocusNames=YGL089C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6306574; DOI=10.1093/nar/11.12.4049;
RA Singh A., Chen E.Y., Lugovoy J.M., Chang C.N., Hitzeman R.A., Seeburg P.H.;
RT "Saccharomyces cerevisiae contains two discrete genes coding for the alpha-
RT factor pheromone.";
RL Nucleic Acids Res. 11:4049-4063(1983).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9290212;
RX DOI=10.1002/(sici)1097-0061(19970915)13:11<1077::aid-yea152>3.0.co;2-y;
RA Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.;
RT "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae
RT chromosome VII.";
RL Yeast 13:1077-1090(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP PROTEIN SEQUENCE OF THE ACTIVE FACTOR.
RA Stoetzler D., Kiltz H.-H., Duntze W.;
RT "Primary structure of alpha-factor peptides from Saccharomyces
RT cerevisiae.";
RL Eur. J. Biochem. 69:397-400(1976).
RN [6]
RP PROTEIN SEQUENCE OF THE ACTIVE FACTOR.
RX PubMed=340452; DOI=10.1093/oxfordjournals.jbchem.a131864;
RA Tanaka T., Kita H., Murakami T., Narita K.;
RT "Purification and amino acid sequence of mating factor from Saccharomyces
RT cerevisiae.";
RL J. Biochem. 82:1681-1687(1977).
CC -!- FUNCTION: The active factor is excreted into the culture medium by
CC haploid cells of the alpha mating type and acts on cells of the
CC opposite mating type (type A). It mediates the conjugation process
CC between the two types by inhibiting the initiation of DNA synthesis in
CC type a cells and synchronizing them with type alpha.
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DR EMBL; X01582; CAA25739.1; -; Genomic_DNA.
DR EMBL; X01582; CAA25740.1; ALT_SEQ; Genomic_DNA.
DR EMBL; X01582; CAA25741.1; ALT_SEQ; Genomic_DNA.
DR EMBL; Z72611; CAA96795.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA08017.1; -; Genomic_DNA.
DR PIR; S05791; S05791.
DR RefSeq; NP_011426.1; NM_001180954.1.
DR AlphaFoldDB; P32435; -.
DR BioGRID; 33162; 14.
DR STRING; 4932.YGL089C; -.
DR iPTMnet; P32435; -.
DR PaxDb; P32435; -.
DR EnsemblFungi; YGL089C_mRNA; YGL089C; YGL089C.
DR GeneID; 852791; -.
DR KEGG; sce:YGL089C; -.
DR SGD; S000003057; MF(ALPHA)2.
DR VEuPathDB; FungiDB:YGL089C; -.
DR eggNOG; ENOG502S0T3; Eukaryota.
DR GeneTree; ENSGT00940000176792; -.
DR HOGENOM; CLU_136956_0_0_1; -.
DR InParanoid; P32435; -.
DR OMA; WHWLRFN; -.
DR BioCyc; YEAST:G3O-34080-MON; -.
DR PRO; PR:P32435; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P32435; protein.
DR GO; GO:0005576; C:extracellular region; TAS:SGD.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:1903135; F:cupric ion binding; IDA:SGD.
DR GO; GO:0000772; F:mating pheromone activity; IDA:SGD.
DR GO; GO:0007618; P:mating; IEA:InterPro.
DR GO; GO:0000750; P:pheromone-dependent signal transduction involved in conjugation with cellular fusion; IMP:SGD.
DR InterPro; IPR016326; Mating_factor_a.
DR InterPro; IPR006742; Mating_factor_alpha_C.
DR InterPro; IPR008675; Mating_factor_alpha_N.
DR Pfam; PF04648; MF_alpha; 2.
DR Pfam; PF05436; MF_alpha_N; 1.
DR PIRSF; PIRSF001866; Mating_factor_alpha; 1.
PE 1: Evidence at protein level;
KW Cleavage on pair of basic residues; Direct protein sequencing; Pheromone;
KW Reference proteome; Repeat; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT PROPEP 22..86
FT /id="PRO_0000419677"
FT PEPTIDE 87..99
FT /note="Mating factor alpha-like"
FT /id="PRO_0000021702"
FT PROPEP 102..107
FT /id="PRO_0000419678"
FT PEPTIDE 108..120
FT /note="Mating factor alpha"
FT /id="PRO_0000021703"
SQ SEQUENCE 120 AA; 13271 MW; 10BF3FDB985FBB2D CRC64;
MKFISTFLTF ILAAVSVTAS SDEDIAQVPA EAIIGYLDFG GDHDIAFLPF SNATASGLLF
INTTIAEAAE KEQNTTLAKR EAVADAWHWL NLRPGQPMYK REANADAWHW LQLKPGQPMY
