ID   MFAP1_CHICK             Reviewed;         442 AA.
AC   P55080;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Microfibrillar-associated protein 1;
DE   AltName: Full=Associated microfibril protein {ECO:0000303|PubMed:1374398};
DE            Short=AMF;
DE   AltName: Full=Spliceosome B complex protein MFAP1 {ECO:0000250|UniProtKB:P55081};
GN   Name=MFAP1 {ECO:0000250|UniProtKB:P55081};
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC   Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC   Phasianinae; Gallus.
OX   NCBI_TaxID=9031;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC   STRAIN=White leghorn; TISSUE=Embryo;
RX   PubMed=1374398; DOI=10.1016/s0021-9258(19)50203-4;
RA   Horrigan S.K., Rich C.B., Streeten B.W., Li Z.-Y., Foster J.A.;
RT   "Characterization of an associated microfibril protein through recombinant
RT   DNA techniques.";
RL   J. Biol. Chem. 267:10087-10095(1992).
CC   -!- FUNCTION: Involved in pre-mRNA splicing as a component of the
CC       spliceosome. {ECO:0000250|UniProtKB:P55081}.
CC   -!- SUBUNIT: Component of the spliceosome B complex. Interacts with PRPF38A
CC       (via N-terminal interaction domain). {ECO:0000250|UniProtKB:P55081}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P55081}.
CC   -!- TISSUE SPECIFICITY: Widely expressed. {ECO:0000269|PubMed:1374398}.
CC   -!- SIMILARITY: Belongs to the MFAP1 family. {ECO:0000305}.
CC   -!- CAUTION: Was initially identified as a component of the elastin-
CC       associated microfibrils (PubMed:1374398). More recent results indicate
CC       it is instead part of the spliceosome B complex.
CC       {ECO:0000250|UniProtKB:P55081, ECO:0000269|PubMed:1374398}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M76679; -; NOT_ANNOTATED_CDS; mRNA.
DR   PIR; A42670; A42670.
DR   AlphaFoldDB; P55080; -.
DR   SMR; P55080; -.
DR   PaxDb; P55080; -.
DR   PRIDE; P55080; -.
DR   VEuPathDB; HostDB:geneid_415586; -.
DR   eggNOG; KOG1425; Eukaryota.
DR   InParanoid; P55080; -.
DR   PhylomeDB; P55080; -.
DR   Proteomes; UP000000539; Unplaced.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0071005; C:U2-type precatalytic spliceosome; ISS:UniProtKB.
DR   GO; GO:0005684; C:U2-type spliceosomal complex; IBA:GO_Central.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR   InterPro; IPR033194; MFAP1.
DR   InterPro; IPR009730; MFAP1_C.
DR   PANTHER; PTHR15327; PTHR15327; 1.
DR   Pfam; PF06991; MFAP1; 1.
PE   2: Evidence at transcript level;
KW   mRNA processing; mRNA splicing; Nucleus; Reference proteome; Spliceosome.
FT   CHAIN           1..442
FT                   /note="Microfibrillar-associated protein 1"
FT                   /id="PRO_0000096457"
FT   REGION          1..34
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          113..203
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..21
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        133..147
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        148..170
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        171..195
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   442 AA;  51964 MW;  D3E92436DE7DF908 CRC64;
     MSAPSALVKQ PPIQSTAGAC PSRNEKGRAV YGEGEGETVC VGKAAADYVP MESSEEEDEE
     FQFIKKAKEQ EVEPEEQEEE VANDPRLRRL LQNRITEDVE ERLARHRKIV EPEVVSGESD
     SEVEGEAWRV EREDTSEEEE EEIDDEEIER WRGMMRQRAQ ERKTEELEVM ELEDEGRSGE
     ESELESEYEE YTDSEDEMEP RLKPVFIRKK DRITVQEREA EALKQKELEQ EAKRLAEERR
     KYTLKIVEEE AKKELEENKR SLAALDALDT DDENDEEEYE AWKVRELKRI KRDREEREAM
     EKEKAEIERM RNLTEEERRA ELRANGKVVT NKAVKGKYKF LQKYYHRGAF FMDEDEEVYK
     RDFSAPTLED HFNKTILPKV MQVKNFGRSG RTKYTHLVDQ DTTSFDSAWG QESAQNTKFF
     KQKAAGVRDV FERPSAKKRK TT